Skip to main content
SpringerLink
Log in

Follicular ADAMTS-1 and aggrecan levels in polycystic ovary syndrome

  • Reproductive Physiology and Disease
  • Published:
Journal of Assisted Reproduction and Genetics Aims and scope Submit manuscript

Abstract

Purpose

Polycystic ovary syndrome (PCOS) is the most common cause of ovulatory dysfunction and female infertility. The etiopathogenetic mechanisms of PCOS have been studied for many years, although exact causes remain unclear. It has been demonstrated that proteoglycan degradation by a disintegrin-like metalloproteinase with thrombospondin type motifs-1 (ADAMTS-1) is essential for ovulation and fertilization. The objective of our study is to analyze the levels of ADAMTS-1 and aggrecan in the follicular fluid (FF) of PCOS patients compared with normal ovulatory women and to determine whether these markers could be a predictor of in vitro fertilization (IVF) success in PCOS patients.

Methods

Women with PCOS (n = 21) and normal ovulatory controls (n = 22) undergoing IVF treatment were recruited. ADAMTS-1 and aggrecan levels were analyzed with enzyme-linked immunosorbent assay (ELISA) and compared between PCOS and normal ovulatory controls. The predictor effect of ADAMTS-1 and aggrecan on fertilization rate and implantation was evaluated.

Results

FF ADAMTS-1 and aggrecan levels increased in women with PCOS compared to controls. Elevated ADAMTS-1 levels but not aggrecan were related to increased implantation in PCOS.

Conclusion

Our study demonstrated that altered levels of ADAMTS-1 and aggrecan may have a partial role in the etiopathogenesis of PCOS, and ADAMTS-1 could be a predictive marker for implantation success in PCOS patients.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Similar content being viewed by others

References

  1. Manneras-Holm L, Leonhardt H, Kullberg J, Jennische E, Odén A, Holm G, et al. Adipose tissue has aberrant morphology and function in PCOS: enlarged adipocytes and low serum adiponectin, but not circulating sex steroids, are strongly associated with insulin resistance. J Clin Endocrinol Metab. 2011;96:304–11.

    Article  Google Scholar 

  2. Li R, Zhang Q, Yang D, Li S, Lu S, Wu X, et al. Prevalence of polycystic ovary syndrome in women in China: a large community-based study. Hum Reprod. 2013;28:2562–9.

    Article  PubMed  Google Scholar 

  3. Corbett S, Morin-Papunen L. The polycystic ovary syndrome and recent human evolution. Mol Cell Endocrinol. 2013;373:39–50.

    Article  CAS  PubMed  Google Scholar 

  4. Yasuo T, Yamaguchi T, Kitaya K. Progesterone induction of chondroitin sulfate proteoglycan aggrecan expression in human endometrial epithelial cells. J Steroid Biochem Mol Biol. 2010;122:159–63.

    Article  CAS  PubMed  Google Scholar 

  5. Yanagishita M, Hascall VC. Biosynthesis of proteoglycans by rat granulosa cells cultured in vitro. J Biol Chem. 1979;254:12355–64.

    CAS  PubMed  Google Scholar 

  6. Mueller PL, Schreiber JR, Lucky AW, Schulman JD, Rodbard D, Ross GT. Follicle-stimulating hormone stimulates ovarian synthesis of proteoglycans in the estrogen-stimulated hypophysectomized immature female rat. Endocrinology. 1978;102:824–31.

    Article  CAS  PubMed  Google Scholar 

  7. Yanagishita M. Proteoglycans and hyaluronan in female reproductive organs. EXS. 1994;70:179–90.

    CAS  PubMed  Google Scholar 

  8. Stanton H, Melrose J, Little CB, Fosang AJ. Proteoglycan degradation by the ADAMTS family of proteinases. Biochim Biophys Acta. 1812;2011:1616–29.

    Google Scholar 

  9. Russell DL, Robker RL. Molecular mechanisms of ovulation: co-ordination through the cumulus complex. Hum Reprod Update. 2007;13:289–312.

    Article  CAS  PubMed  Google Scholar 

  10. Brown HM, Dunning KM, Robker RL, Boerboom D, Pritchard M, Lane M, et al. ADAMTS1 cleavage of versican mediates essential structural remodeling of the ovarian follicle and cumulus-oocyte matrix during ovulation in mice. Biol Reprod. 2010;83:549–57.

    Article  CAS  PubMed  Google Scholar 

  11. Sandy JD, Westling J, Kenagy RD, Iruela-Arispe ML, Verscharen C, Rodriguez-Mazaneque JC, et al. Versican V1 proteolysis in human aorta in vivo occurs at the Glu441-Ala442 bond, a site that is cleaved by recombinant ADAMTS-1 and ADAMTS-4. J Biol Chem. 2001;276:13372–8.

    Article  CAS  PubMed  Google Scholar 

  12. Xiao S, Li Y, Chen M, Xu Y, Wen Y, Zhou C. Evidence for decreased expression of ADAMTS-1 associated with impaired oocyte quality in PCOS patients. J Clin Endocrinol Metab. 2014;99:1015–21.

    Article  Google Scholar 

  13. Brown HM, Dunning KR, Robker RL, Pritchard M, Russell DL. Requirement for ADAMTS-1 in extracellular matrix remodeling during ovarian folliculogenesis and lymphangiogenesis. Dev Biol. 2006;300:699–709.

    Article  CAS  PubMed  Google Scholar 

  14. Doyle KM, Russell DL, Sriraman V, Richards JS. Coordinate transcription of the ADAMTS-1 gene by luteinizing hormone and progesterone receptor. Mol Endocrinol. 2004;18:2463–78.

    Article  CAS  PubMed  Google Scholar 

  15. Boerboom D, Russell DL, Richards JS, Sirois J. Regulation of transcripts encoding ADAMTS-1 (a disintegrin and metalloproteinase with thrombospondin-like motifs-1) and progesterone receptor by human chorionic gonadotropin in equine preovulatory follicles. J Mol Endocrinol. 2003;31:473–85.

    Article  CAS  PubMed  Google Scholar 

  16. Russell, Ochsner SA, Hsieh M, Mulders S, Richards JS. Hormone-regulated expression and localization of versican in the rodent ovary. Endocrinology. 2003;144:1020–31.

    Article  CAS  PubMed  Google Scholar 

  17. Russell DL, Salustri A. Extracellular matrix of the cumulus-oocyte complex. Semin Reprod Med. 2006;24:217–27.

    Article  CAS  PubMed  Google Scholar 

  18. Yung Y, Maman E, Konopnicki S, Cohen B, Brengauz M, Lojkin I, et al. ADAMTS-1: a new human ovulatory gene and a cumulus marker for fertilization capacity. Mol Cell Endocrinol. 2010;328:104–8.

    Article  CAS  PubMed  Google Scholar 

  19. Eriksen GV, Malmstrom A, Uldbjerg N. Human follicular fluid proteoglycans in relation to in vitro fertilization. Fertil Steril. 1997;68(5):791–8.

    Article  CAS  PubMed  Google Scholar 

  20. Bellin ME, Ax RL, Laufer N, Tarlatzis BC, DeCherney AH, Feldberg D, et al. Glycosaminoglycans in follicular fluid from women undergoing in vitro fertilization and their relationship to cumulus expansion, fertilization, and development. Fertil Steril. 1986;45:244–8.

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgements

E.N.T. formulated the present hypothesis and was responsible for writing the report. D.U.K. and B.O. collected the data and M.E. was responsible for analyzing the data. N.K. was responsible for statistical analysis.

Author information

Authors and Affiliations

  1. Faculty of Medicine, Department of Gynecology and Obstetrics, In vitro Fertilization Unit, Suleyman Demirel University, Isparta, Turkey

    Esra Nur Tola & Hilmi Baha Oral

  2. Faculty of Medicine, In vitro Fertilization Unit, Suleyman Demirel University, Isparta, Turkey

    Dilek Ulusoy Karatopuk

  3. Department of Gynecology and Obstetrics, Kanuni Sultan Suleyman Training and Research Hospital, Istanbul, Turkey

    Nadiye Koroglu

  4. Department of Biochemistry, Gaziantep 25 Aralık State Hospital, Gaziantep, Turkey

    Merve Ergin

Authors
  1. Esra Nur Tola
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Dilek Ulusoy Karatopuk
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Nadiye Koroglu
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Merve Ergin
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Hilmi Baha Oral
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Esra Nur Tola.

Ethics declarations

Conflict of interest

The authors declare that they have no conflict of interest.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Tola, E.N., Karatopuk, D.U., Koroglu, N. et al. Follicular ADAMTS-1 and aggrecan levels in polycystic ovary syndrome. J Assist Reprod Genet 34, 811–816 (2017). https://doi.org/10.1007/s10815-017-0913-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10815-017-0913-7

Keywords

Search

Navigation