Journal of Assisted Reproduction and Genetics

, Volume 34, Issue 2, pp 239–244 | Cite as

Morphokinetics of vitrified and warmed blastocysts predicts implantation potential

  • T. EbnerEmail author
  • P. Oppelt
  • E. Radler
  • C. Allerstorfer
  • A. Habelsberger
  • R. B. Mayer
  • O. Shebl
Embryo Biology



It was studied whether morphokinetics of blastocoele re-expansion and hatching in vitrified-warmed blastocysts is predictive of implantation, clinical pregnancy, and live birth.


In 144 patients aiming for single warmed blastocyst transfer, blastocysts were cultured in a new time-lapse system (Miri® TL) immediately after warming. Video sequences with an image interval of 5 min were annotated and the corresponding morphokinetic variables were correlated with pregnancy outcome. In detail, tRE (start of re-expansion), tCRE (completion of re-expansion), tAH (hatching from the manipulated zona pellucida), and presence of collapses were recorded.


In the pregnant group, tRE and tCRE were significantly lower (0.69 ± 0.45 h and 2.16 ± 0.94 h) as compared to the non-pregnant group (1.23 ± 1.08 h and 2.70 ± 1.20 h). Both variables and the duration of re-expansion (tCRE-tRE) allowed for distinction between “non-pregnant,” “loss of pregnancy,” and live birth/ongoing pregnancy. Presence and number of collapses showed no correlation with outcome.


Time-lapse imaging of vitrified-warmed blastocysts offers additional selection criteria allowing for prediction of implantation potential. As a consequence, cumulative pregnancy rate could be increased and time-to-pregnancy reduced.


Blastocyst vitrification Blastocyst re-expansion Morphokinetics Time-lapse imaging Hatching 



The authors would like to thank G. Schappacher-Tilp for statistical expertise.


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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • T. Ebner
    • 1
    Email author
  • P. Oppelt
    • 1
  • E. Radler
    • 1
  • C. Allerstorfer
    • 1
  • A. Habelsberger
    • 1
  • R. B. Mayer
    • 1
  • O. Shebl
    • 1
  1. 1.Department of Gynecology, Obstetrics and Gynecological EndocrinologyKepler University ClinicLinzAustria

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