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Journal of Assisted Reproduction and Genetics

, Volume 34, Issue 2, pp 253–261 | Cite as

Associations between IVF outcomes and essential trace elements measured in follicular fluid and urine: a pilot study

  • Mary E. Ingle
  • Michael S. BloomEmail author
  • Patrick J. Parsons
  • Amy J. Steuerwald
  • Pamela Kruger
  • Victor Y. Fujimoto
Reproductive Physiology and Disease

Abstract

Purpose

A hypothesis-generating pilot study exploring associations between essential trace elements measured in follicular fluid (FF) and urine and in vitro fertilization (IVF) endpoints.

Methods

We recruited 58 women undergoing IVF between 2007 and 2008, and measured cobalt, chromium, copper, manganese, molybdenum, and zinc in FF (n = 46) and urine (n = 45) by inductively coupled plasma mass spectrometry (ICP-MS). We used multivariable regression models to assess the impact of FF and urine trace elements on IVF outcomes, adjusted for age, body mass index, race, and cigarette smoking.

Results

Trace elements were mostly present at lower concentrations in FF than in urine. The average number of oocytes retrieved was positively associated with higher urine cobalt, chromium, copper, and molybdenum concentrations. FF chromium and manganese were negatively associated with the proportion of mature oocytes, yet urine manganese had a positive association. FF zinc was inversely associated with average oocyte fertilization. Urine trace elements were significant positive predictors for the total number of embryos generated. FF copper predicted lower embryo fragmentation while urine copper was associated with higher embryo cell number and urine manganese with higher embryo fragmentation. No associations were detected for implantation, pregnancy, or live birth.

Conclusions

Our results suggest the importance of trace elements in both FF and urine for intermediate, although not necessarily clinical, IVF endpoints. The results differed using FF or urine biomarkers of exposure, which may have implications for the design of clinical and epidemiologic investigations. These initial findings will form the basis of a more definitive future study.

Keywords

Essential trace elements In vitro fertilization (IVF) Biomarkers Follicular fluid Urine 

Notes

Compliance with ethical standards

Conflict of interest

The authors declare they have no conflicts of interest.

Supplementary material

10815_2016_853_MOESM1_ESM.doc (46 kb)
Table S1 (DOC 45.5 kb)
10815_2016_853_MOESM2_ESM.doc (50 kb)
Table S2 (DOC 50 kb)

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Mary E. Ingle
    • 1
  • Michael S. Bloom
    • 1
    • 2
    • 3
    Email author
  • Patrick J. Parsons
    • 2
    • 4
  • Amy J. Steuerwald
    • 4
  • Pamela Kruger
    • 4
  • Victor Y. Fujimoto
    • 5
  1. 1.Department of Epidemiology and BiostatisticsUniversity at Albany, State University of New YorkRensselaerUSA
  2. 2.Department of Environmental Health SciencesUniversity at Albany, State University of New YorkRensselaerUSA
  3. 3.School of Public HealthUniversity at Albany, State University of New YorkRensselaerUSA
  4. 4.Laboratory of Inorganic and Nuclear Chemistry, Wadsworth CenterNew York State Department of HealthAlbanyUSA
  5. 5.Department of Obstetrics, Gynecology, and Reproductive SciencesUniversity of California at San FranciscoSan FranciscoUSA

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