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Proteomic profile of maternal-aged blastocoel fluid suggests a novel role for ubiquitin system in blastocyst quality

  • Embryo Biology
  • Published:
Journal of Assisted Reproduction and Genetics Aims and scope Submit manuscript

Abstract

Purpose

The etiology of maternal aging, a common cause of female factor infertility and a rate-limiting step in vitro fertilization (IVF) success, remains still unclear. Proteomic changes responsible for the impaired successful pregnancy outcome after IVF with aged blastocysts have not been yet evaluated. The objective of this prospective study was to employ proteomic techniques and bioinformatic tools to enlight differences at the protein level in blastocoel fluid of aged and younger woman.

Methods

Protein composition of human blastocoel fluid isolated by micromanipulation from 46 blastocysts of women aged <37 years (group A) and 29 of women aged ≥37 years (group B) have been identified by a shotgun proteomic approach based on high-resolution nano-liquid chromatography electrospray-ionization-tandem mass spectrometry (nLC-ESI-MS/MS) using label free for the relative quantification of their expression levels.

Results

The proteomic analysis leads to the identification and quantification of 148 proteins; 132 and 116 proteins were identified in groups A and B, respectively. Interestingly, the identified proteins are mainly involved in processes aimed at fine tuning embryo implantation and development. Among the 100 proteins commonly expressed in both groups, 17 proteins are upregulated and 44 downregulated in group B compared to group A. Overall, the analysis identified 33 proteins, which were increased or present only in B while 76 were decreased in B or present only in A.

Conclusions

Data revealed that maternal aging mainly affects blastocyst survival and implantation through unbalancing the equilibrium of the ubiquitin system known to play a crucial role in fine-tuning several aspects required to ensure successful pregnancy outcome.

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Acknowledgements

We thank Dr. Francesca Grassi Scalvini and Dr. Fabiana Santagata for their skillful technical assistance.

Authors’ roles

G.T., E.A., V.P., A.B., and P.E.L.S. designed the research. G.T., E.A., V.P., A.B., E.M., A.N., and S.N. performed the research. G.T., E.A., V.P., A.B., E.M.B., E.M., A.N., S.N., and M.M. analyzed the data. G.T., E.A., V.P., A.B., E.M.B., and M.M. wrote the paper.

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    Authors and Affiliations

    1. Dipartimento di Medicina Veterinaria, Università degli Studi di Milano, Milan, Italy

      Gabriella Tedeschi, Armando Negri & Simona Nonnis

    2. Fondazione Filarete, 20139, Milan, Italy

      Gabriella Tedeschi & Elisa Maffioli

    3. Humanitas Fertility Center, Department of Gynecology, Division of Gynecology and Reproductive Medicine, Humanitas Research Hospital, Via Manzoni 56, Rozzano, 20089, Milan, Italy

      Elena Albani, Valentina Parini, Anna Maria Brucculeri & Paolo Emanuele Levi-Setti

    4. Dipartimento di Biotecnologie Mediche e Medicina Traslazionale, Università degli Studi di Milano, Milan, Italy

      Elena Monica Borroni

    5. Department of Medicine, Campus Bio-Medico University of Rome, Via Alvaro del Portillo 21, 00128, Rome, Italy

      Mauro Maccarrone

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    1. Gabriella Tedeschi
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    Correspondence to Elena Monica Borroni.

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    Maternal aging mainly affects blastocyst survival and implantation through unbalancing the equilibrium of theubiquitin system

    Gabriella Tedeschi and Elena Albani contributed equally to this work.

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    Tedeschi, G., Albani, E., Borroni, E.M. et al. Proteomic profile of maternal-aged blastocoel fluid suggests a novel role for ubiquitin system in blastocyst quality. J Assist Reprod Genet 34, 225–238 (2017). https://doi.org/10.1007/s10815-016-0842-x

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