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Protein expression in human cumulus cells as an indicator of blastocyst formation and pregnancy success

  • Assisted Reproduction Technologies
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Abstract

Purpose

The goal for the present study was to implement a technique for protein extraction and identification in human cumulus cells (CCs).

Methods

Forty samples of CCs were collected after ovum pick-up from patients undergoing intracytoplasmic sperm injection (ICSI). Samples were split into the blastocyst group (n = 10), including patients in which all embryos converted into blastocysts, and the non-blastocyst group (n = 10), including patients in which none of the embryos reached the blastocyst stage or the positive-pregnancy (n = 10) and negative-pregnancy group (n = 10). Proteins were extracted and injected into a liquid chromatography system coupled to a mass spectrometer. The spectra were processed and used to search a database.

Results

There were 87 different proteins in samples from the blastocyst and non-blastocyst groups, in which 30 were exclusively expressed in the blastocyst group and 17 in the non-blastocyst group. Among the 72 proteins detected in the pregnancy groups, 19 were exclusively expressed in the positive, and 16 were exclusively expressed in the negative-pregnancy group.

Conclusions

CC proteomics may be useful for predicting pregnancy success and the identification of patients that should be included in extended embryo culture programs.

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Acknowledgments

This study was supported by Fundação de Amparo à Pesquisa do Estado de São Paulo, FAPESP, a Brazilian founding agency: 2013/50052-7.

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Authors and Affiliations

  1. Fertility Medical Group, Av. Brigadeiro Luis Antônio, 4545, São Paulo, SP, 01401-002, Brazil

    Daniela Paes Almeida Ferreira Braga, Amanda Souza Setti, Rita Cássia Sávio Figueira & Edson Borges Jr.

  2. Disciplina de Urologia, Área de Reprodução Humana, Departamento de Cirurgia, Universidade Federal de São Paulo—UNIFESP, Rua Embaú, 231, São Paulo, SP, 04039-060, Brazil

    Daniela Paes Almeida Ferreira Braga, Edson Guimarães Lo Turco, Fernanda Bertuccez Cordeiro & Edson Borges Jr.

  3. Instituto Sapientiae—Centro de Estudos e Pesquisa em Reprodução Humana Assistida, Rua Vieira Maciel, 62, São Paulo, SP, 04503-040, Brazil

    Daniela Paes Almeida Ferreira Braga, Amanda Souza Setti, Sylvia Sanches Cortezzi & Erika Ono

  4. Centro Pluridisciplinar de Pesquisas Químicas, Biológicas e Agronômicas (CPQBA)-Universidade de Campinas—UNICAMP, Campinas, SP, 13083-970, Brazil

    Elaine Cristina Cabral

  5. Laboratório ThoMSon de Espectrometria de Massas—Instituto de Química, Universidade de Campinas—UNICAMP, Campinas, SP, 13083-970, Brazil

    Marcos Nogueira Eberlin

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  1. Daniela Paes Almeida Ferreira Braga
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  2. Amanda Souza Setti
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  3. Edson Guimarães Lo Turco
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  4. Fernanda Bertuccez Cordeiro
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  10. Edson Borges Jr.
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CC proteomics may be useful for predicting pregnancy success and the identification of patients that should be included in extended embryo culture programs.

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Braga, D.P.A.F., Setti, A.S., Lo Turco, E.G. et al. Protein expression in human cumulus cells as an indicator of blastocyst formation and pregnancy success. J Assist Reprod Genet 33, 1571–1583 (2016). https://doi.org/10.1007/s10815-016-0800-7

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