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Journal of Assisted Reproduction and Genetics

, Volume 33, Issue 9, pp 1231–1238 | Cite as

Reduced sperm DNA longevity is associated with an increased incidence of still born; evidence from a multi-ovulating sequential artificial insemination animal model

  • Stephen D. JohnstonEmail author
  • Carmen López-Fernández
  • Francisca Arroyo
  • Altea Gosálbez
  • Elva I. Cortés Gutiérrez
  • Jose-Luis Fernández
  • Jaime Gosálvez
Reproductive Physiology and Disease

Abstract

Purpose

Using a rabbit model, we assessed the influence of sperm DNA longevity on female reproductive outcomes.

Methods

Semen was collected from 40 bucks, incubated at 38 °C for 24 h, and the rate of sperm DNA fragmentation (rSDF) was determined using the sperm chromatin dispersion assay. Males were allocated into high rSDF (>0.5 units of increase per hour) or low rSDF (<0.5 units of increase per hour) groups. High and low rSDF semen samples were sequentially artificially inseminated into the same doe to reduce female factor variability, and pregnancy outcomes were recorded.

Results

While there was no difference in SDFs between rSDF groups immediately after collection (T0), differences were significant after 2 h of incubation; SDFs determined at collection and rSDF behaved as independent characters (Pearson correlation = 0.099; P = 0.542). Following artificial insemination, the rate of stillborn pups was significantly higher in does inseminated by males with a high rSDF (14/21) compared to those with low rSDF (15/6); (contingency χ2 5.19; p = 0.022). The risk of stillborn when low rSDF rabbits were used for insemination was 0.16, but increased to 0.36 when high rSDF animals were used (odds ratio = 2.85; 95 % confidence interval = 1.4–2.7).

Conclusion(s)

Dynamic assessment of SDF coupled with natural multiple ovulation, high fecundity of the rabbit and control over female factor influence, provided a useful experimental model to demonstrate the adverse effect of reduced sperm DNA longevity on reproductive outcome.

Keywords

Oryctolagus cuniculus Sperm DNA fragmentation Still born Sperm DNA longevity Dynamic assay Animal model 

Notes

Acknowledgments

This research was supported by the Spanish Ministry of Economy and Competitiveness, MINECO (BFU-2013-44290-R).

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Stephen D. Johnston
    • 1
    Email author
  • Carmen López-Fernández
    • 2
  • Francisca Arroyo
    • 2
  • Altea Gosálbez
    • 2
  • Elva I. Cortés Gutiérrez
    • 3
  • Jose-Luis Fernández
    • 4
  • Jaime Gosálvez
    • 2
  1. 1.School of Agriculture and Food ScienceThe University of QueenslandGattonAustralia
  2. 2.Faculty of BiologyAutonomous University of MadridCantoblancoSpain
  3. 3.Department of Genetics, Northeastern Biomedical Research CentreThe Mexican Social Security InstituteMonterreyMexico
  4. 4.Unidad de GenéticaComplejo Hospitalario Universitario A Coruña (CHUAC)-INIBIC and Centro Oncológico de GaliciaLa CoruñaSpain

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