Reduced sperm DNA longevity is associated with an increased incidence of still born; evidence from a multi-ovulating sequential artificial insemination animal model
- 184 Downloads
Using a rabbit model, we assessed the influence of sperm DNA longevity on female reproductive outcomes.
Semen was collected from 40 bucks, incubated at 38 °C for 24 h, and the rate of sperm DNA fragmentation (rSDF) was determined using the sperm chromatin dispersion assay. Males were allocated into high rSDF (>0.5 units of increase per hour) or low rSDF (<0.5 units of increase per hour) groups. High and low rSDF semen samples were sequentially artificially inseminated into the same doe to reduce female factor variability, and pregnancy outcomes were recorded.
While there was no difference in SDFs between rSDF groups immediately after collection (T0), differences were significant after 2 h of incubation; SDFs determined at collection and rSDF behaved as independent characters (Pearson correlation = 0.099; P = 0.542). Following artificial insemination, the rate of stillborn pups was significantly higher in does inseminated by males with a high rSDF (14/21) compared to those with low rSDF (15/6); (contingency χ2 5.19; p = 0.022). The risk of stillborn when low rSDF rabbits were used for insemination was 0.16, but increased to 0.36 when high rSDF animals were used (odds ratio = 2.85; 95 % confidence interval = 1.4–2.7).
Dynamic assessment of SDF coupled with natural multiple ovulation, high fecundity of the rabbit and control over female factor influence, provided a useful experimental model to demonstrate the adverse effect of reduced sperm DNA longevity on reproductive outcome.
KeywordsOryctolagus cuniculus Sperm DNA fragmentation Still born Sperm DNA longevity Dynamic assay Animal model
This research was supported by the Spanish Ministry of Economy and Competitiveness, MINECO (BFU-2013-44290-R).
- 1.Drobnis EZ, Johnson MH. Are we ready to incorporate sperm DNA-fragmentation testing into our male infertility work-up? A plea for more robust studies. Reprod Biomed Online. 2015;30:111–2.Google Scholar
- 8.Gosálvez J, López-Fernández C, Fernández JL, Gouraud A, Holt WV. Relationships between the dynamics of iatrogenic DNA damage and genomic design in mammalian sperm from eleven species. Mol Reprod Dev. 2011;78:951–61.Google Scholar
- 10.Gosálvez J, López-Fernández C, Arroyo F, Gosálbez A, Gutiérrez-Cortés El, Johnston SD. The assessment of sperm DNA damage in rabbits using the Halomax assay. In: Adamo G, Constanza A, editors. Rabbits: biology, diet and eating habits and disorders. New York: NOVA Science publishers; 2013. p. 87–100.Google Scholar
- 12.Mocé E, Vicente JS, Lavara R. Effect of donor strain and maturation stage of rabbit oocytes on results of penetration test of rabbit semen. World Rabbit Sci. 2002;10:53–62.Google Scholar
- 13.Gosálvez J, López-Fernández C, Fernández JL, Esteves SC, Johnston SD. Unpacking the mysteries of sperm DNA fragmentation: ten frequently asked questions. J Reprod Biotech Fertil. 2015;4:1–16.Google Scholar
- 16.Wdowiak A, Bojar I. Relationship between pregnancy, embryo development, and sperm deoxyribonucleic acid fragmentation dynamics. Saudi J Biol Sci. 2015 (in press). Published online ahead of print 10 August 2015. doi: 10.1016/j.sjbs.2015.08.001.
- 18.Leach M, Aitken RJ, Sacks G. Sperm DNA fragmentation abnormalities in men from couples with a history of recurrent miscarriage. Aust NZ J Obst Gyn. 2015;55:379–83.Google Scholar
- 20.Genescà A, Caballín MR, Miró R, Benet J, Germà JR, Egozcue J. Repair of human sperm chromosome aberrations in the hamster egg. Hum Genet. 1992;89:181–6.Google Scholar
- 21.Derijck A, van der Heijden G, Giele M, Philippens M, de Boer P. DNA double-strand break repair in parental chromatin of mouse zygotes, the first cell cycle as an origin of de novo mutation. Hum Mol Genet. 2008;17:1922–37.Google Scholar
- 22.Marchetti F, Bishop J, Gingerich J, Wyrobek AJ. Meiotic interstrand DNA damage escapes paternal repair and causes chromosomal aberrations in the zygote by maternal misrepair. Sci Rep. 2015;5:7689.Google Scholar
- 26.Fernández-Gonzalez R, Moreira PN, Pérez-Crespo M, Sánchez-Martín M, Ramırez MA, Pericuesta E, et al. Long-term effects of mouse intracytoplasmic sperm injection with DNA fragmented sperm on health and behavior of adult offspring. Biol Reprod. 2008;78:761–72.Google Scholar