Journal of Assisted Reproduction and Genetics

, Volume 33, Issue 9, pp 1223–1230 | Cite as

Can Coenzyme Q10 supplementation protect the ovarian reserve against oxidative damage?

  • Pınar ÖzcanEmail author
  • Cem Fıçıcıoğlu
  • Ozge Kizilkale
  • Mert Yesiladali
  • Olgu Enis Tok
  • Ferda Ozkan
  • Mukaddes Esrefoglu
Reproductive Physiology and Disease



We investigated antioxidant effects of CoQ10 supplementation on the prevention of OS-induced ovarian damage and to evaluate the protective effect of such supplementation against OS-related DNA damage.


Twenty-four adult female Sprague–Dawley rats were randomly divided into three groups (8 rats per group): group 1 (control): saline, ip, and orally; group 2 (cisplatin group): cisplatin, 4.5 mg/kg ip, two times with an interval of 7 days; and group 3 (cisplatin + CoQ10 group): cisplatin, 4.5 mg/kg ip, two times with an interval of 7 days, and 24 h before cisplatin, 150 mg/kg/day orally in 1 mL of saline daily for 14 days. Serum concentrations of anti-Mullerian hormone (AMH), number of AMH-positive follicles, the assessment of the intensity of 8'OHdG immunoreactivity, the primordial, antral and atretic follicle counts in the ovary were assessed.


The mean serum AMH concentrations were 1.3 ± 0.19, 0.16 ± 0.03, and 0.27 ± 0.20 ng/mL in groups 1, 2, and 3, respectively (p < 0.01). Serum AMH levels were significantly higher in group 1 compared to groups 2 and 3 (p < 0.01 and p = 0.01, respectively). There was a statistically significant difference in AMH-positive follicle count between the groups (p < 0.01). Group 1 showed higher numbers of AMH-positive granulosa cells compared to group 2 (p = 0.01). A significant difference was found in the primordial, the atretic, and antral follicle counts between the three groups (p < 0.01, p < 0.01, and p < 0.01, respectively). The atretic follicle count was significantly lower in the cisplatin plus CoQ10 group compared to the cisplatin group (p < 0.01). The antral follicle counts were significantly higher in the cisplatin plus CoQ10 group compared with the cisplatin group (p < 0.01). There was a statistically significant difference in the intensity of staining of the follicles that were positive for anti-8'OHdG between the groups (p = 0.02). Group 1 showed a significant lower intensity of staining of the follicles positive for anti-8'OHdG compared with group 2 (p = 0.03).


CoQ10 supplementation may protect ovarian reserve by counteracting both mitochondrial ovarian ageing and physiological programmed ovarian ageing although the certain effect of OS in female infertility is not clearly known.


AMH Ovarian reserve Oxidative damage 8'OHdG Coenzyme Q10 


Compliance with ethical standards

The study protocol was approved by the Institutional Animal Care and Use Committee of Yeditepe University. All procedures were performed in accordance with the National Academy of Science’s Guide for Care and Use of Laboratory Animals (1996).

Conflicts of interest

The authors declare that they have no conflict of interest.


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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Pınar Özcan
    • 1
    • 2
    Email author
  • Cem Fıçıcıoğlu
    • 3
  • Ozge Kizilkale
    • 3
  • Mert Yesiladali
    • 3
  • Olgu Enis Tok
    • 4
  • Ferda Ozkan
    • 5
  • Mukaddes Esrefoglu
    • 4
  1. 1.Department of Obstetrics and GynecologyBezmialem University Faculty of MedicineİstanbulTurkey
  2. 2.Division of Reproductive Endocrinology and Infertility, Department of Obstetrics, Gynecology and Reproductive BiologyBrigham and Women’s Hospital, Harvard Medical SchoolBostonUSA
  3. 3.Department of Obstetrics and Gynecology, Faculty of MedicineYeditepe UniversityIstanbulTurkey
  4. 4.Department of Histology and EmbryologyBezmialem University Faculty of MedicineİstanbulTurkey
  5. 5.Department of Pathology, Faculty of MedicineYeditepe UniversityIstanbulTurkey

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