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Outcomes of intracytoplasmic sperm injection using the zona pellucida-bound sperm or manually selected sperm

  • Rui JinEmail author
  • Junhua BaoEmail author
  • Dawei Tang
  • Fang Liu
  • Guoping Wang
  • Yaqiong Zhao
  • Gang Bai
  • Yongjie Liu
  • Ying Wang
  • Lili Liu
  • Jing Zhao
Assisted Reproduction Technologies

Abstract

Purpose

Zona pellucida (ZP)-bound sperm used for intracytoplasmic sperm injection (ICSI) enhances embryo quality, implantation, and clinical pregnancy rates. This study aimed to assess the pregnancy outcomes and clinical significance of ICSI with ZP-bound sperm.

Method

A total of 84 infertile couples who underwent cycles of ICSI following failed in vitro fertilization between June 2012 and February 2014 were enrolled and randomized (1:1): in the treatment group, ICSI was performed using ZP-bound sperm; in the control group, ICSI was performed in a standard manner. Rates of fertilization, cleavage, high-quality embryos, and clinical pregnancy were compared between the two groups.

Results

There were no significant differences in age, infertile period, gonadotrophin dose, number of metaphase II oocytes, and number of embryo transfers between the two groups (P > 0.05). The clinical pregnancy rate was higher in the treatment group than in the control group, but without statistical significance (60.5 vs. 47.6 %, P > 0.05). No significant differences in the rates of fertilization and cleavage were observed (83.0 vs. 81.6 %, and 96.3 vs. 96.5 %, both P > 0.05), but higher rates of high-quality embryos and useable embryos were observed with ZP-bound sperm compared with controls (66.1 vs. 50.8 % and 76.0 vs. 66.3 %, both P < 0.05).

Conclusions

ICSI using ZP-bound sperm might increase the embryo quality and number of useable embryos, possibly improving the clinical pregnancy outcome of ICSI.

Keywords

In vitro fertilization Intracytoplasmic sperm injection Sperm-zona pellucida binding test Embryo transfer Clinical pregnancy rate Fertilization rate Cleavage rate High-quality embryo rate 

Notes

Acknowledgments

We are grateful to all the participating patients of this study. We thank the staff members of this trial, our colleagues, and all the study staff for their enormous efforts in collecting and ensuring the accuracy and completeness of all the data.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

All procedures performed in studies involving human participants were in accordance with the ethical standards of the Ethical Committees of Maternal and Children Health Care Hospital of Yinchuan and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

Informed consent

Informed consent was obtained from all individual participants included in the study.

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Rui Jin
    • 1
    Email author
  • Junhua Bao
    • 1
    Email author
  • Dawei Tang
    • 1
  • Fang Liu
    • 1
  • Guoping Wang
    • 1
  • Yaqiong Zhao
    • 1
  • Gang Bai
    • 1
  • Yongjie Liu
    • 1
  • Ying Wang
    • 1
  • Lili Liu
    • 1
  • Jing Zhao
    • 1
  1. 1.Centre of Assisted ReproductionMaternal and Children Health Care Hospital of YinchuanYinchuanChina

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