Molecular analysis of DNA in blastocoele fluid using next-generation sequencing
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Preimplantation genetic testing (PGT) requires an invasive biopsy to obtain embryonic material for genetic analysis. The availability of a less invasive procedure would increase the overall efficacy of PGT. The aim of the study was to explore the potential of blastocoele fluid (BF) as an alternative source of embryonic DNA for PGT.
Collection of BF was performed by aspiration with a fine needle prior to vitrification. BF DNA was subjected to whole-genome amplification (WGA) and analyzed by high-resolution next-generation sequencing (NGS).
A high-quality WGA product was obtained from 8 of 11 (72.7 %) samples. Comparison of matching BF and blastomere samples showed that the genomic representation of sequencing reads was consistently similar with respect to density and regional coverage across the 24 chromosomes. A genome-wide survey of the sample sequencing data also indicated that BF was highly representative of known single gene sequences, and this observation was validated by PCR analyses of ten randomly selected genes, with an overall efficiency of 84 %.
This study provides further evidence that BF is a promising alternative source of DNA for PGT.
KeywordsBlastocoele fluid Blastomere Next-generation sequencing Preimplantation genetic testing Bioinformatics
The study was supported by the grant awarded to Yuanqing Yao by the Key Program of the “Twelfth Five-Year Plan” of the People’s Liberation Army (No. BWS11J058) and the National High Technology Research and Development Program (2015AA020402).
Compliance with ethical standards
This study was approved by the Institutional Review Board of Chinese PLA General Hospital (S2013-092-02). All embryos donated to research were obtained after obtaining informed written consent by couples undertaking PGS.
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