A combination of hydroxypropyl cellulose and trehalose as supplementation for vitrification of human oocytes: a retrospective cohort study
This study aimed to determine whether the new formulation of vitrification solutions containing a combination of hydroxypropyl cellulose (HPC) and trehalose does not affect outcomes in comparison with using conventional solutions made of serum substitute supplement (SSS) and sucrose.
Ovum donation cycles were retrospectively compared regarding the solution used for vitrification and warming of human oocytes. The analysis included 218 cycles (N = 2532 oocytes) in the study group (HPC + trehalose) and 214 cycles (N = 2353 oocytes) in the control group (SSS + sucrose).
No statistical differences were found in ovarian stimulation parameters and baseline characteristics of donors and recipients. The survival rate was 91.3 % (95 % confidence interval (CI) = 89.8–92.9) in the HPC + trehalose group vs. 92.1 % (95 % CI = 90.4–93.7) in the SSS + sucrose group (NS). The implantation rate (42.8 %, 95 % CI = 37.7–47.9 vs. 41.2 %, 95 % CI = 36.0–46.4), clinical pregnancy rate (CPR) per transfer (60.7 %, 95 % CI = 53.9–67.5 vs. 56.4 %, 95 % CI = 49.3–63.5), and ongoing pregnancy rate (OPR) per transfer (48.5 %, 95 % CI = 41.5–55.5 vs. 46.3 %, 95 % CI = 39.2–53.4) were similar for patients who received either HPC + trehalose-vitrified oocytes or SSS + sucrose-vitrified oocytes. Statistical differences were found when analyzing blastocyst rate both per injected oocyte (30.2 %, 95 % CI = 28.3–32.1 vs. 24.1 %, 95 % CI = 22.3–25.9) and per fertilized oocyte (40.8 %, 95 %CI = 38.5–43.1 vs. 33.2 %, 95 % CI = 30.8–35.5) (P < 0.0001). Delivery rate was comparable between groups (37.2 %, 95 % CI = 30.8–46.6 vs. 36.9 %, 95 % CI = 30.4–43.4; NS).
Our data demonstrate that HPC and trehalose are suitable and safe substitutes for serum and sucrose. Therefore, the new commercial media can be used efficiently in the vitrification of human oocytes avoiding viral and endotoxin contamination risk.
KeywordsHydroxypropyl cellulose Macromolecular supplement Oocyte vitrification Survival rate Clinical outcome
The authors wish to thank the clinical embryologists and laboratory technicians of IVI, Valencia.
- 10.Herrero L, Pareja S, Losada C, Cobo AC, Pellicer A, Garcia-Velasco JA. Avoiding the use of human chorionic gonadotropin combined with oocyte vitrification and GnRH agonist triggering versus coasting: a new strategy to avoid ovarian hyperstimulation syndrome. Fertil Steril. 2011;95:1137–40.CrossRefPubMedGoogle Scholar
- 14.Inoue F. Hydroxypropyl cellulose as a macromolecular supplement for cryopreservation of bovine oocytes and blastocysts and human oocytes. Low Temp Med. 2012;38.Google Scholar
- 18.Vanin S, Bubacco L, Beltramini M. Seasonal variation of trehalose and glycerol concentrations in winter snow-active insects. Cryo Lett. 2008;29:485–91.Google Scholar
- 23.Zhang XB, Li K, Yau KH, Tsang KS, Fok TF, Li CK, et al. Trehalose ameliorates the cryopreservation of cord blood in a preclinical system and increases the recovery of CFUs, long-term culture-initiating cells, and nonobese diabetic-SCID repopulating cells. Transfusion. 2003;43:265–72.CrossRefPubMedGoogle Scholar
- 26.Ardoy M, Calderón G, Arroyo G, Cuadros J, Figueroa M, Herrer R. ASEBIR criteria for the morphological evaluation of human oocytes, early embryos and blastocysts. ASEBIR clinical embryology papers. 2008.Google Scholar
- 28.Gardner DKSW. In vitro culture of human blastocysts. In: Jansen R, Mortimer D, editors. Toward reproductive certainty: infertility and genetics beyond 1999. Carnforth: Parthenon Press; 1999.Google Scholar
- 29.Zegers-Hochschild F, Adamson GD, de Mouzon J, Ishihara O, Mansour R, Nygren K, et al. International Committee for Monitoring Assisted Reproductive Technology (ICMART) and the World Health Organization (WHO) revised glossary of ART terminology, 2009. Fertil Steril. 2009;92:1520–4.CrossRefPubMedGoogle Scholar