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Multiple thrombophilic single nucleotide polymorphisms lack a significant effect on outcomes in fresh IVF cycles: an analysis of 1717 patients

  • Genetics
  • Published:
Journal of Assisted Reproduction and Genetics Aims and scope Submit manuscript

Abstract

Purpose

The aim of the study is to determine if thrombophilic single nucleotide polymorphisms (SNPs) affect outcomes in fresh in vitro fertilization (IVF) cycles in a large general infertility population.

Methods

A prospective cohort analysis was performed at a university-affiliated private IVF center of female patients undergoing fresh non-donor IVF cycles. The effect of the following thrombophilic SNPs on IVF outcomes were explored: factor V (Leiden and H1299R), prothrombin (G20210A), factor XIII (V34L), β-fibrinogen (-455G → A), plasminogen activator inhibitor-1 (4G/5G), human platelet antigen-1 (a/b9L33P), and methylenetetrahydrofolate reductase (C677T and A1298C). The main outcome measures included positive pregnancy test, clinical pregnancy, embryo implantation, live birth, and pregnancy loss.

Results

Patients (1717) were enrolled in the study, and a total of 4169 embryos were transferred. There were no statistically significant differences in positive pregnancy test, clinical pregnancy, embryo implantation, live birth, or pregnancy loss in the analysis of 1717 patients attempting their first cycle of IVF. Receiver operator characteristics and logistic regression analyses showed that outcomes cannot be predicted by the cumulative number of thrombophilic mutations present in the patient.

Conclusions

Individual and cumulative thrombophilic SNPs do not affect IVF outcomes. Therefore, initial screening for these SNPs is not indicated.

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Acknowledgments

Ferring Pharmaceuticals (Saint-Prex, Switzerland) financially supported this study with an unrestricted research grant. The authors greatly appreciated the help of Batsal Devkota and Deanne Taylor for assistance with the database queries.

Author information

Authors and Affiliations

  1. National Institutes of Health, Bethesda, MD, USA

    George Patounakis

  2. Mount Sinai School of Medicine, New York, NY, USA

    Eric Bergh

  3. Reproductive Medicine Associates of New Jersey, 140 Allen Road, Basking Ridge, Morristown, NJ, 07920, USA

    Eric J. Forman, Xin Tao, Agnieszka Lonczak, Jason M. Franasiak, Nathan Treff & Richard T. Scott Jr.

  4. Rutgers Robert Wood Johnson Medical School, Piscataway, NJ, USA

    Eric J. Forman, Jason M. Franasiak, Nathan Treff & Richard T. Scott Jr.

Authors
  1. George Patounakis
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  2. Eric Bergh
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  3. Eric J. Forman
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  4. Xin Tao
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  5. Agnieszka Lonczak
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  6. Jason M. Franasiak
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  7. Nathan Treff
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  8. Richard T. Scott Jr.
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Corresponding author

Correspondence to Richard T. Scott Jr..

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Conflict of interest

The authors of this paper have no conflicts of interest, financial or otherwise, to declare.

Additional information

Clinical Trial Number (ClinicalTrials.gov): NCT01223092

Capsule

Nine thrombophilic single nucleotide polymorphisms lack a significant effect on positive pregnancy test, clinical pregnancy, embryo implantation, pregnancy loss, and live birth in fresh non-donor IVF cycles.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplemental Table 1

Custom TaqMan SNP Genotyping Assay primers and probes (DOC 55 kb)

Supplemental Table 2

Genotype frequencies compared with HapMap CEU population (DOC 57 kb)

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Patounakis, G., Bergh, E., Forman, E.J. et al. Multiple thrombophilic single nucleotide polymorphisms lack a significant effect on outcomes in fresh IVF cycles: an analysis of 1717 patients. J Assist Reprod Genet 33, 67–73 (2016). https://doi.org/10.1007/s10815-015-0606-z

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  • DOI: https://doi.org/10.1007/s10815-015-0606-z

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