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Morphokinetics analysis of embryos derived from vitrified/warmed oocytes

  • Assisted Reproduction Technologies
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Abstract

Purpose

Oocyte vitrification is a worldwide used technique that has proved its worth. Although it was shown not to alter oocyte integrity, a recent study concluded that it may affect oocyte embryo development. As the morphology and kinetics of embryos derived from sibling fresh and vitrified oocytes have not been described previously, the present study evaluates cleavage rate, blastomeres size, fragmentation rate, and blastocyst formation in vitrified/warmed oocyte derived embryos (VODE) as compared with sibling fresh oocytes derived embryos (FODE).

Methods

This investigation included 90 infertility cases displaying large cohort of mature oocytes at pick up, divided into 2 groups after denudation. A part of oocytes underwent ICSI while others were vitrified. Oocyte warming cycles were performed when no pregnancy was achieved using fresh eggs. Zygote to blastocyst development was recorded prospectively in an image database up to day 5.

Results

VODE did not show major difference as compared with FODE in terms of cleavage rate, number of blastomeres, fragmentation rate, and blastomeres size. Furthermore, percentage of morulae at day 4 and blastocysts at day 5 are not affected by oocyte vitrification.

Conclusion

Although our results show that embryo development is not altered by oocyte vitrification, offspring follow-up is essential to exclude any adverse developmental effect of the technique.

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Acknowledgments

We acknowledge François Lecluyse and Anne-Laure Benedetti for technical support. We would also like to thank clinical staff for addressing the couples to us and for allowing their participation in the present study.

Conflict of interest

The authors declare that they have no competing interests.

Funding

This research did not receive any specific grant from any funding agency in the public, commercial or not-for-profit sector.

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Authors and Affiliations

  1. Service de Médecine et Biologie de la Reproduction, Hôpital Saint-Joseph, 26 boulevard de Louvain, 13008, Marseille, France

    D. Montjean, C. Geoffroy-Siraudin, M. Gervoise-Boyer, P. Tourame & P. Boyer

Authors
  1. D. Montjean
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  2. C. Geoffroy-Siraudin
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  3. M. Gervoise-Boyer
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  4. P. Tourame
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  5. P. Boyer
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Corresponding author

Correspondence to D. Montjean.

Additional information

Capsule

This is the first report on morphologic and morphokinetic parameters in embryos derived from sibling oocytes that were either vitrified/warmed prior to fertilization or fertilized shortly after pick up. The present data show that the development of embryos obtained from vitrified/warmed oocytes is not significantly different from embryos formed from sibling fresh non-vitrified oocytes.

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Montjean, D., Geoffroy-Siraudin, C., Gervoise-Boyer, M. et al. Morphokinetics analysis of embryos derived from vitrified/warmed oocytes. J Assist Reprod Genet 32, 1615–1621 (2015). https://doi.org/10.1007/s10815-015-0569-0

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  • DOI: https://doi.org/10.1007/s10815-015-0569-0

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