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Analysis of the correlation of CATSPER single nucleotide polymorphisms (SNPs) with idiopathic asthenospermia

  • Genetics
  • Published:
Journal of Assisted Reproduction and Genetics Aims and scope Submit manuscript

Abstract

Purpose

Idiopathic asthenospermia is the most common type of male infertility. Although the mechanisms causing asthenospermia are complex, recent studies have indicated an important role of cation channel of sperm (CATSPER) gene downregulation or abnormality in the etiology of idiopathic asthenospermia.

Methods

In the present study, 192 patients with idiopathic asthenospermia and 288 healthy controls were enrolled, and a flight mass spectrometry using Sequenom’s MassArray biochip system was applied for genotyping 16 CATSPER gene SNPs reported in the human single nucleotide polymorphism (SNP) database.

Results

Our results indicated a correlation between CATSPER1 SNPs and idiopathic asthenospermia. In particular, the exonal SNP rs1893316 in CATSPER1 significantly correlated with idiopathic asthenospermia risk and is a potential important factor in determining an individual’s genetic susceptibility to idiopathic asthenospermia.

Conclusion

These finding will help to further elucidate the role of CATSPER1 in idiopathic asthenospermia pathogenesis.

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References

  1. Brugh VM, Lipshultz LI. Male factor infertility: evaluation and management. Med Clin North Am. 2004;88(2):367–85.

    Article  PubMed  Google Scholar 

  2. Tamburrino L, Marchiani S, Minetti F, Forti G, Muratori M, Baldi E. The CatSper calcium channel in human sperm: relation with motility and involvement in progesterone-induced acrosome reaction. Hum Reprod. 2014;29(3):418–28.

    Article  CAS  PubMed  Google Scholar 

  3. Quill TA, Ren D, Clapham DE, Garbers DL. A voltage-gated ion channel expressed specifically in spermatozoa. Proc Natl Acad Sci U S A. 2001;98(22):12527–31.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  4. Ren D, Navarro B, Perez G, Jackson AC, Hsu S, Shi Q, et al. A sperm ion channel required for sperm motility and male fertility. Nature. 2001;413(6856):603–9.

    Article  CAS  PubMed  Google Scholar 

  5. Quill TA, Sugden SA, Rossi KL, Doolittle LK, Hammer RE, Garbers DL. Hyperactivated sperm motility driven by CatSper2 is required for fertilization. Proc Natl Acad Sci U S A. 2003;100(25):14869–74.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  6. Jin JL, O'Doherty AM, Wang S, Zheng H, Sanders KM, Yan W. Catsper3 and catsper4 encode two cation channel-like proteins exclusively expressed in the testis. Biol Reprod. 2005;73(6):1235–42.

    Article  CAS  PubMed  Google Scholar 

  7. Qi H, Moran MM, Navarro B, Chong JA, Krapivinsky G, Krapivinsky L, et al. All four CatSper ion channel proteins are required for male fertility and sperm cell hyperactivated motility. Proc Natl Acad Sci U S A. 2007;104(4):1219–23.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  8. Nikpoor P, Mowla SJ, Movahedin M, Ziaee SA, Tiraihi T. CatSper gene expression in postnatal development of mouse testis and in subfertile men with deficient sperm motility. Hum Reprod. 2004;19(1):124–8.

    Article  PubMed  Google Scholar 

  9. Mohammadi S, Movahedin M, Mowla SJ. Up-regulation of CatSper genes family by selenium. Reprod Biol Endocrinol. 2009;7:126.

    Article  PubMed Central  PubMed  Google Scholar 

  10. Sachidanandam R, Weissman D, Schmidt SC, Kakol JM, Stein LD, Marth G, et al. A map of human genome sequence variation containing 1.42 million single nucleotide polymorphisms. Nature. 2001;409(6822):928–33.

    Article  CAS  PubMed  Google Scholar 

  11. Yu Q, Zhang Y, Xia Y, Yang X, Li N, Ye L, et al. Analysis of endothelial nitric oxide synthase (eNOS) G894T polymorphism and semen parameters in a Chinese Han population. Andrologia. 2014;46(5):541–6.

    Article  CAS  PubMed  Google Scholar 

  12. Carlson AE, Westenbroek RE, Quill T, Ren D, Clapham DE, Hille B, et al. CatSper1 required for evoked Ca2+ entry and control of flagellar function in sperm. Proc Natl Acad Sci U S A. 2003;100(25):14864–8.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  13. Kirichok Y, Navarro B, Clapham DE. Whole-cell patch-clamp measurements of spermatozoa reveal an alkaline-activated Ca2+ channel. Nature. 2006;439(7077):737–40.

    Article  CAS  PubMed  Google Scholar 

  14. Xia J, Ren D. Egg coat proteins activate calcium entry into mouse sperm via CATSPER channels. Biol Reprod. 2009;80(6):1092–8.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  15. Li HG, Liao AH, Ding XF, Zhou H, Xiong CL. The expression and significance of CATSPER1 in human testis and ejaculated spermatozoa. Asian J Androl. 2006;8(3):301–6.

    Article  PubMed  Google Scholar 

  16. Wang YN, Wang B, Liang M, Han CY, Zhang B, Cai J, et al. Down-regulation of CatSper1 channel in epididymal spermatozoa contributes to the pathogenesis of asthenozoospermia, whereas up-regulation of the channel by Sheng-Jing-San treatment improves the sperm motility of asthenozoospermia in rats. Fertil Steril. 2013;99(2):579–87.

    Article  PubMed  Google Scholar 

  17. Buldreghini E, Mahfouz RZ, Vignini A, Mazzanti L, Ricciardo-Lamonica G, Lenzi A, et al. Single nucleotide polymorphism (SNP) of the endothelial nitric oxide synthase (eNOS) gene (Glu298Asp variant) in infertile men with asthenozoospermia. J Androl. 2010;31(5):482–8.

    Article  CAS  PubMed  Google Scholar 

  18. Tronchon V, Vialard F, El Sirkasi M, Dechaud H, Rollet J, Albert M, et al. Tumor necrosis factor-alpha-308 polymorphism in infertile men with altered sperm production or motility. Hum Reprod. 2008;23(12):2858–66.

    Article  CAS  PubMed  Google Scholar 

  19. Visser L, Westerveld GH, Xie F, van Daalen SK, van der Veen F, Lombardi MP, et al. A comprehensive gene mutation screen in men with asthenozoospermia. Fertil Steril. 2011;95(3):1020–4. e1021-1029.

    Article  CAS  PubMed  Google Scholar 

  20. Avenarius MR, Hildebrand MS, Zhang Y, Meyer NC, Smith LL, Kahrizi K, et al. Human male infertility caused by mutations in the CATSPER1 channel protein. Am J Hum Genet. 2009;84(4):505–10.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  21. Hildebrand MS, Avenarius MR, Fellous M, Zhang Y, Meyer NC, Auer J, et al. Genetic male infertility and mutation of CATSPER ion channels. Eur J Hum Genet. 2010;18(11):1178–84.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  22. Bhilawadikar R, Zaveri K, Mukadam L, Naik S, Kamble K, Modi D, et al. Levels of Tektin 2 and CatSper 2 in normozoospermic and oligoasthenozoospermic men and its association with motility, fertilization rate, embryo quality and pregnancy rate. J Assist Reprod Genet. 2013;30(4):513–23.

    Article  PubMed Central  PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Urology, NanFang Hospital, Southern Medical University, No. 1838, North Guangzhou Avenue, Guangzhou, 510515, Guangdong Province, China

    Fangpeng Shu, Xumin Zhou, Daojun Lu, Bin Lei, Qi Li, Yu Yang & Xiangming Mao

  2. School of Biotechnology, Southern Medical University, No. 1838, North Guangzhou Avenue, Guangzhou, 510515, Guangdong Province, China

    Fenxia Li & Xuexi Yang

  3. Institute of Genetic Engineering, Southern Medical University, No. 1838, North Guangzhou Avenue, Guangzhou, 510515, Guangdong Province, China

    Rong Shi

Authors
  1. Fangpeng Shu
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  2. Xumin Zhou
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  3. Fenxia Li
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  4. Daojun Lu
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  5. Bin Lei
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  6. Qi Li
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  7. Yu Yang
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  8. Xuexi Yang
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  9. Rong Shi
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  10. Xiangming Mao
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Corresponding authors

Correspondence to Xuexi Yang, Rong Shi or Xiangming Mao.

Additional information

Capsule The exonal SNP rs1893316 in CATSPER1 significantly correlated with idiopathic asthenospermia risk and is a potential important factor in determining an individual’s genetic susceptibility to idiopathic asthenospermia.

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Cite this article

Shu, F., Zhou, X., Li, F. et al. Analysis of the correlation of CATSPER single nucleotide polymorphisms (SNPs) with idiopathic asthenospermia. J Assist Reprod Genet 32, 1643–1649 (2015). https://doi.org/10.1007/s10815-015-0548-5

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  • DOI: https://doi.org/10.1007/s10815-015-0548-5

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