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Journal of Assisted Reproduction and Genetics

, Volume 32, Issue 9, pp 1359–1364 | Cite as

Association of WNT4 polymorphisms with endometriosis in infertile patients

  • Fernanda Mafra
  • Michele Catto
  • Bianca Bianco
  • Caio Parente Barbosa
  • Denise Christofolini
Genetics

Abstract

Purpose

Recently, several genome-wide association studies have demonstrated an association between endometriosis and markers located in or near to WNT4 gene. In order to assess the validity of the findings, we conducted a replication case–control study in a Brazilian population.

Methods

Genetic association study comprising 400 infertile women with endometriosis and 400 fertile women as controls. TaqMan allelic discrimination technique was used to investigate the relationship between endometriosis and four single-nucleotide polymorphisms (rs16826658, rs3820282, rs2235529, and rs7521902) in WNT4 gene. Genotype distribution, allele frequency, and haplotype analysis of the WNT4 polymorphisms were performed. A p value <0.05 was considered significant.

Results

The results revealed a significant association of rs16826658 (p = 7e-04) and rs3820282 (p = 0.048) single-nucleotide polymorphisms (SNPs) on WNT4 gene with endometriosis-related infertility, while rs2235529 and rs7521902 SNPs showed no difference between cases and controls.

Conclusions

Our results suggested that rs16826658 and rs3820282 polymorphisms on WNT4 gene might be involved in the pathogenesis of endometriosis in the infertile women studied. Analysis of WNT4 genetic variants might help to identify patients at high risk for disease development.

Keywords

Endometriosis WNT4 gene Polymorphism Infertility 

Notes

Acknowledgments

The work was supported by a grant from Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) No. 2013/14470-9. F.M. was supported by a PhD scholarship from FAPESP No. 2012/22394-8.

Conflict of interest

The authors declare that they have no competing interests.

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Fernanda Mafra
    • 1
  • Michele Catto
    • 1
  • Bianca Bianco
    • 1
  • Caio Parente Barbosa
    • 1
  • Denise Christofolini
    • 1
    • 2
  1. 1.Collective Health Department, Division of Reproductive Health and Population GeneticsFaculdade de Medicina do ABCSanto AndréBrazil
  2. 2.Av. Príncipe de Gales, 821, CEPESSanto AndréBrazil

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