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Journal of Assisted Reproduction and Genetics

, Volume 32, Issue 7, pp 1113–1121 | Cite as

Ploidy of spermatogenic cells of men with non-mosaic Klinefelter’s syndrome as measured by a computerized cell scanning system

  • Alisa Komsky-Elbaz
  • Arieh Raziel
  • Ido Ben-Ami
  • Orna Bern
  • Billa Maslansky
  • Yariv S. Gidoni
  • Raphael Ron-El
  • Deborah Strassburger
Genetics

Abstract

Purpose

This study aims to characterize the origin of testicular post-meiotic cells in non-mosaic Klinefelter’s syndrome (KS).

Methods

The study included testicular tissue specimens from 11 non-mosaic KS patients, with (6 positive) and without (5 negative) spermatozoa presence. The obtained testicular cells were affixed and stained for morphology followed by fluorescence in situ hybridization (FISH) for centromeric probes X, Y, and 18. We used a computerized automated cell scanning system that enables simultaneous viewing of morphology and FISH in the same cell.

Results

A total of 12,387 cells from the positive cases, 11,991 cells from the negative cases, and 1,711 cells from the controls were analyzed. The majority of spermatogonia were 47, XXY in both the positive and negative KS cases (88.9 ± 4.76 % and 90.6 ± 4.58 %) as were primary spermatocytes (76.8 ± 8.14 % and 79.6 ± 7.30 %). The respective rates of secondary spermatocytes and post-meiotic cells (round, elongating spermatids and sperm cells) were 1.1 ± 1.39 % in the positive cases, 2.9 ± 3.33 % in the negative cases, compared to 67.6 ± 6.22 % in the controls (P < 0.02). Pairing of both 18 and XY homologous chromosomes in 46,XY primary spermatocytes was 2.5 ± 2.31 % and 3.4 ± 2.39 %, respectively, compared to 19.8 ± 8.95 % in the control group (P < 0.02) and in 47,XXY primary spermatocytes in 2.4 ± 3.8 % in the positive group and 3.2 ± 2.26 % in the negative group.

Conclusions

This study presents data to indicate that the majority of primary spermatocytes in the testes of non-mosaic KS patients are 47,XXY and could possibly develop into post-meiotic cells.

Keyword

Ploidy Testicular suspension Klinefelter’s syndrome Spermatogenic cells 

Notes

Acknowledgments

The authors thank Prof Altarescu from Shaare Zedek Medical Center, Jerusalem, for reviewing the manuscript; Ms. Esti Kasterstein and Ms. Daphna Komarovsky from the IVF Unit at Assaf Harofeh Medical, Zerifin, Israel, and Ms. Tal Kaplan from BioView Ltd, Nes Ziona, Israel, for their professional contribution; Ms. Fredrica Gendler from Assaf Harofeh Medical Center, Zerifin, Israel, for her linguistic editing and commitment to the study and Ms. Esther Eshkol for her editorial assistance.

Conflict of interest

The authors declare that they have no conflict of interest.

Funding

There are no sources of funding or financial support.

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Alisa Komsky-Elbaz
    • 1
  • Arieh Raziel
    • 1
  • Ido Ben-Ami
    • 1
  • Orna Bern
    • 1
  • Billa Maslansky
    • 1
  • Yariv S. Gidoni
    • 1
  • Raphael Ron-El
    • 1
  • Deborah Strassburger
    • 1
  1. 1.Infertility and IVF UnitAssaf Harofeh Medical CenterZerifinIsrael

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