Systemic oxidative stress could predict assisted reproductive technique outcome
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Previous studies have indicated that OxS (oxidative stress) may appear as a possible reason for poor ART outcome. Our aim was to study OxS levels in both partners of couples seeking Assisted reproduction Technology (ART).
Altogether 79 couples were recruited. Oxidative DNA damage (8-OHdG) and lipid peroxidation (8-EPI) were measured, and clinical background and ART outcomes were recorded.
Both OxS markers accurately reflected clincal conditions with prominent negative effects attributable to genital tract infections, endometriosis, uterine myoma and smoking. Furthermore, the level of OxS was also affected by partner’s state of health. The highest 8-EPI levels were detected in both partners when biochemically detectable pregnancies did not develop into clinically detectable pregnancies (in women, 97,8 ± 16,7 vs 72.9 ± 22,9, p = 0.007; in men, 89.6 ± 20,4 vs 72,1 ± 22,6, p = 0.049).
To conclude, high grade systemix OxS in both partners may negatively affect the maintenance and outcome of pregnancy. Applying the detection of OxS in ART patients may select patients with higher success rate and/or those who require antioxidant therapy. This would lead to improvement of ART outcome as well as natural fertility.
Keywords8-EPI (8-iso Prostaglandin F2α) 8-OHdG (8-hydroxy-2’-deoxyguanosine) ART (assisted reproductive technique) Infertility OxS (oxidative stress) ROS (reactive oxygen species)
The present study was supported by Enterprise Estonia (Grant no. EU 30020), Estonian Ministry of Education and Research (Target Financing SF0180132s08, Institutional Research Funding IUT 20–42, Institutional Research Funding IUT 15–19 and Scientific Collection Financing KOGU-HUMB) and University of Tartu (Grant no. SARMBARENG).
Conflict of interest
The authors declare that they have no conflict of interest.
- 1.Zegers-Hochschild F, Adamson GD, de Mouzon J, Ishihara O, Mansour R, Nygren K, et al. International Committee for Monitoring Assisted Reproductive Technology (ICMART) and the World Health Organization (WHO) revised glossary of ART terminology. Fertil Steril. 2009;92:1520–4.Google Scholar
- 2.National Health and Medical Research Council. Ethical guidelines on assisted reproductive technology. Available at: http://www.nhmrc.gov.au/_files_nhmrc/publications/attachments/e28.pdf. Accessed 2 Feb 2014.
- 12.Meseguer M, Martínez-Conejero JA, O’Connor JE, Pellicer A, Remohí J, Garrido N. The significance of sperm DNA oxidation in embryo development and reproductive outcome in an oocyte donation program: a new model to study a male infertility prognostic factor. Fertil Steril. 2008;89:1191–9.CrossRefPubMedGoogle Scholar
- 17.WHO. WHO laboratory manual for the examination and processing of human semen. 5th ed. Geneva: World Health Organization; 2010.Google Scholar
- 18.WHO, editor. Laboratory manual for examination of human semen and sperm–cervical mucus interaction. 4th ed. New York: Cambridge University Press; 1999.Google Scholar
- 21.Halliwell B, Gutteridge JMC. Free radicals in biology and medicine. New York: Oxford University Press; 1999.Google Scholar
- 22.Sies H. Oxidative stress: oxidants and antioxidants. London: Academic; 1991.Google Scholar