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Downregulation of gene expression and activity of GRIM-19 affects mouse oocyte viability, maturation, embryo development and implantation

  • Gamete Biology
  • Published:
Journal of Assisted Reproduction and Genetics Aims and scope Submit manuscript

Abstract

Purpose

To investigate the expression of GRIM-19 (Gene associated with retinoid-interferon-induced mortality 19) in mouse oocytes and preimplantation embryos, and to study the effect of GRIM-19 on the developmental competence of mouse oocytes and embryos.

Methods

GRIM-19 was evaluated at both mRNA and protein levels. The expression of GRIM-19 gene was downregulated in mouse oocytes cultured in vitro by specific small interfering RNA (siRNA) injection, while the activity of GRIM-19 was decreased by microinjection of a GRIM-19 antibody into the cytoplasm of germinal vesicle (GV) oocytes. Oocytes matured in vitro were then fertilized by intracytoplasmic sperm injection (ICSI), followed by observation and evaluation of fertilization rate, cleavage rate, blastocyst formation rate and implantation rate.

Results

GRIM-19 is expressed throughout oocyte maturation and preimplantation embryo development stages. GRIM-19 was localized primarily in the cytoplasm of all cells examined. Downregulation of gene expression and activity of GRIM-19 resulted in decreased oocyte viability, potency of oocyte maturation, embryo development and implantation.

Conclusions

GRIM-19 may play important roles in mouse oogenesis and early embryonic development and implantation.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (grant number 81370711), National Natural Science Foundation of China (grant number 30901603), Doctor Foundation of Shandong Province (grant number BS2009SW031), and Shandong Family Planning Commission Foundation (grant number 2009–8).

Author information

Authors and Affiliations

  1. Infertility Center, Qilu Hospital of Shandong University, Jinan, Shandong, 250012, China

    Lan Chao, Yang Yang, Wenjuan Cui, Jing Xu, Honglei Chen & Xiaohui Deng

  2. Department of breast disease, Qilu Hospital of Shandong University, Jinan, Shandong, China

    Xiao Wang

  3. Department of Histology and Embryology, Shandong University, Jinan, Shandong, China

    Aijun Hao

Authors
  1. Lan Chao
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  2. Xiao Wang
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  3. Yang Yang
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  4. Wenjuan Cui
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  5. Jing Xu
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  6. Honglei Chen
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  7. Aijun Hao
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  8. Xiaohui Deng
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Correspondence to Xiaohui Deng.

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Chao, L., Wang, X., Yang, Y. et al. Downregulation of gene expression and activity of GRIM-19 affects mouse oocyte viability, maturation, embryo development and implantation. J Assist Reprod Genet 32, 461–470 (2015). https://doi.org/10.1007/s10815-014-0413-y

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  • DOI: https://doi.org/10.1007/s10815-014-0413-y

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