Sperm preparation before freezing improves sperm motility and reduces apoptosis in post-freezing-thawing sperm compared with post-thawing sperm preparation
- 384 Downloads
To evaluate whether sperm preparation (swim-up technique) before freezing improves the percentages of sperm motility, sperm viability, and non-apoptotic spermatozoa after freezing-thawing process compared with preparation after cryopreservation.
Semen samples from 65 infertile males were equally divided into two aliquots one of which was processed for swim-up prior to cryopreservation and one of which was processed following cryopreservation. Sperm count, motility, and apoptosis index were measured in each group.
The total sperm count and the total motile sperm count decreased after thawing in both the pre-preparation and non-preparation groups compared with neat semen group (P < 0.001). Moreover, the percentage of apoptotic sperm in the pre-preparation group after cryopreservation was lower than that in the non-preparation group (P < 0.05), whereas the percentage of vital sperm with progressive motility was higher than that in the pre-preparation group (P < 0.001).
Semen preparation by swim-up before freezing resulted in better sperm quality and fewer apoptotic sperm than sperm preparation after thawing. Therefore, sperm preparation before cryopreservation should be considered in routine sperm cryopreservation.
KeywordsApoptosis Cryopreservation Swim-up Motility Vitality
The study was financially supported by the Siriraj Grant for Research and Development, Faculty of Medicine Siriraj Hospital, Mahidol University. I would like to thank Orawan Makemaharn, Suphadtra Phornwilardsiri, Suvana Sangchoo, Sukanya Sriiam, and all of the staff members in the Infertility Unit for their help in conducting the study. Finally, I would like to thank all participants in this research.
Conflict of interests
The study involves no conflict of interests and was financially supported by a Siriraj Grant for Research and Development, Faculty of Medicine Siriraj Hospital, Mahidol University
- 11.Twigg J, Fulton N, Gomez E, Irvine DS, Aitken RJ. Analysis of the impact of intracellular reactive oxygen species generation on the structural and functional integrity of human spermatozoa: lipid peroxidation, DNA fragmentation and effectiveness of antioxidants. Hum Reprod. 1998;13:1429–36.PubMedCrossRefGoogle Scholar
- 18.Brugnon F, Ouchchane L, Pons-Rejraji H, Artonne C, Farigoule M, Janny L. Density gradient centrifugation prior to cryopreservation and hypotaurine supplementation improve post-thaw quality of sperm from infertile men with oligoasthenoteratozoospermia. Hum Reprod. 2013;28:2045–57.PubMedCrossRefGoogle Scholar
- 27.WHO. Cambridge; Cambridge University Press; 2010. WHO laboratory manual for the examination and processing of human semen.Google Scholar