Abstract
Objective
To report a birth of a healthy girl after long-term oocyte cryopreservation by slow cooling in sodium depleted medium.
Design
Clinical application.
Setting
University Affiliated, Private IVF center.
Patient
A 38-year-old woman received embryos from IVF by intracytoplasmic sperm injection (ICSI) with her own oocytes that were cryopreserved by slow freezing in a low-sodium medium 14 years and 6 months before, when she was 24 years old.
Main outcome measure(s)
Survival, fertilization, cleavage, clinical pregnancy and delivery.
Result(s)
From six metaphase-II oocytes thawed, two survived, one was fertilized after ICSI and a cleaving embryo was transferred on day 3. A single term pregnancy was achieved, ending with the delivery of a healthy girl.
Conclusion(s)
Cryopreservation after slow freezing in a sodium depleted medium maintained the developmental competence of oocytes after long-term storage and resulted in a successful live birth. As far as is known, this case represents, up to date, the longest storage period of cryopreserved human oocytes resulting in a live birth.
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References
Donaldson MJ, Quintans CJ, Paganini AM, Bozzini MS, Pasqualini AR, Pasqualini SR. 2 year follow-up of children born after the transfer of embryos obtained by in vitro fertilization of human oocytes cryopreserved in low sodium medium. Fertil Steril. 2008;90 Suppl 1:S246–7.
Quintans CJ, Donaldson MJ, Bertolino MV, Pasqualini RS. Birth of two babies using oocytes that were cryopreserved in a choline-based freezing medium. Hum Reprod. 2002;17:3142–52.
Quintans CJ, Donaldson MJ, Urquiza MF, Carretero I, Pasqualini RA, Horton M, et al. Live birth of twins after IVF of oocytes that were cryopreserved almost 12 years before. Reprod BioMed Online. 2012;25:600–2.
Boldt J. Current results with slow freezing and vitrification of the human oocyte. Reprod BioMed Online. 2011;23:314–22.
Kim TJ, Hong SW. Successful live birth from vitrified oocytes after 5 years of cryopreservation. J Assist Reprod Genet. 2011;28:73–6.
Parmegiani L, Garello C, Granella F, Guidetti D, Bernardi S, Cognigni GE, et al. Long-term cryostorage does not adversely affect the outcome of oocyte thawing cycles. Reprod BioMed Online. 2009;19:374–9.
Yang D, Brown SE, Nguyen K, Reddy V, Brubaker C, Winslow KL. Live birth after the transfer of human embryos developed from cryopreserved oocytes harvested before cancer treatment. Fertil Steril. 2007;87:1469e1–e3.
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The authors declare that they have no conflict of interest.
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Capsule 14 years after retrieval and cryopreservation of a mature oocyte using slow freeze technology, a live birth was obtained substantiating the prospect of long term storage for delaying reproduction in humans.
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Urquiza, M.F., Carretero, I., Cano Carabajal, P.R. et al. Successful live birth from oocytes after more than 14 years of cryopreservation. J Assist Reprod Genet 31, 1553–1555 (2014). https://doi.org/10.1007/s10815-014-0318-9
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DOI: https://doi.org/10.1007/s10815-014-0318-9