Trophectoderm morphology predicts outcomes of pregnancy in vitrified-warmed single-blastocyst transfer cycle in a Chinese population
- 271 Downloads
In this study, we estimated the effect of blastocoele expansion, ICM and TE quality after warming and culture on the rates of clinical pregnancy, live birth and miscarriage in vitrified-warmed single-blastocyst transfer cycle in a Chinese population.
A retrospective analysis of 263 cycles of vitrified-warmed single-blastocyst transfers was performed.
The blastocysts with higher TE grade significantly increased the rates of clinical pregnancy (OR = 0.59, 95 % CI, 0.35–0.99, P = 0.045, grade (A + B) vs grade C) and live birth (OR = 0.55, 95 % CI, 0.32–0.94, P = 0.029, grade (A + B) vs grade C). And the association between TE grade and the rate of live birth didn’t change after the number of repeated cycles was adjusted (OR = 0.55, 95 % CI, 0.32–0.95, P = 0.033, grade (A + B) vs grade C). The number of repeated cycles was a confounding factor significantly different between the live birth and no live birth groups. By contrast, neither blastocoele expansion nor inner cell mass was statistically related to the rates of clinical pregnancy, live birth and miscarriage.
Our data firstly provided the evidence that TE grading, but not ICM grading, was significantly associated with the clinical pregnancy rate and live birth rate in vitrified-warmed blastocyst transfer cycles in a Chinese population. TE morphology may help predict outcomes of pregnancy in single-blastocyst transfer.
KeywordsBlastocyst grading Trophectoderm Vitrified-warmed single-blastocyst transfer Clinical pregnancy Live birth
This study was financially supported by the National Natural Science Foundation of China (grant nos. 81100420 and 81270701), the Foundation of Nanjing Medical University (grant no. 2011NJMU210), the Natural Science Foundation of Jiangsu Province (grant no. BK2012520) and Nanjing Medical Science and Technique Development Foundation (2010NJMU030).
Declaration of interest
The authors declare no conflict of interest.
- 2.Papanikolaou EG, Kolibianakis EM, Tournaye H, Venetis CA, Fatemi H, Tarlatzis B, et al. Live birth rates after transfer of equal number of blastocysts or cleavage-stage embryos in IVF. A Syst Rev Meta-Analysis Hum Reprod. 2008;23:91–9.Google Scholar
- 6.Endo T, Honnma H, Hayashi T, Chida M, Yamazaki K, Kitajima Y, et al. Continuation of GnRH agonist administration for 1 week, after hCG injection, prevents ovarian hyperstimulation syndrome following elective cryopreservation of all pronucleate embryos. Hum Reprod. 2002;17:2548–51.PubMedCrossRefGoogle Scholar
- 11.Gardner DK, Schoolcraft WB. In vitro culture of human blastocyst. In: Mortimer JR, editor. Toward Reproductive Certainty: Infertility and Genetics Beyond 1999. Carnforth, UK: Parthenon Press; 1999. p. 378–88.Google Scholar
- 24.Schmidt CL, de Ziegler D, Gagliardi CL, Mellon RW, Taney FH, Kuhar MJ, et al. Transfer of cryopreserved-thawed embryos: the natural cycle versus controlled preparation of the endometrium with gonadotropin-releasing hormone agonist and exogenous estradiol and progesterone (GEEP). Fertil Steril. 1989;52:609–16.PubMedGoogle Scholar
- 25.Thompson SM, Onwubalili N, Brown K, Jindal SK, McGovern PG. Blastocyst expansion score and trophectoderm morphology strongly predict successful clinical pregnancy and live birth following elective single embryo blastocyst transfer (eSET): a national study. J Assist Reprod Genet. 2013;30:1577–81.PubMedCrossRefGoogle Scholar