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Study of the effect of varicocelectomy on sperm proteins expression in patients with varicocele and poor sperm quality by using two-dimensional gel electrophoresis

  • Hani Hosseinifar
  • Marjan Sabbaghian
  • Davood Nasrabadi
  • Tahereh Modarresi
  • Ahmad Vosough Taqi Dizaj
  • Hamid Gourabi
  • Mohmmad Ali Sadighi Gilani
Gamete Biology

Abstract

Purpose

To compare proteomic profiles of spermatozoa from patients with varicocele and poor sperm quality before and after varicocelectomy.

Methods

This work was designed as a prospective and observational study. The study was based on 20 men with varicocele grade 3 and poor sperm quality undergoing varicocelectomy at the Fertility Unit of Royan institute in 2009. Two semen samples were collected, one before varicocelectomy and the other after surgery. Protein separation was done by two-dimensional protein electrophoresis, and analyzed by gel densitometry and mass spectrometry. Differential sperm protein expression levels were measured by gel densitometry.

Results

Comparison of the sperm parameters showed that sperm motility and concentration were increased after varicocelectomy. At the level of protein, a total of 3 protein spots were identified whose expression was significantly lower in sperm samples before varicocelectomy compared with after surgery including heat shock protein A5 (HSPA5), superoxide dismutase 1 (SOD1) and δ-subunit of the catalytic core of mitochondrial adenosine triphosphate synthase (ATP5D).

Conclusions

High grade varicocoele affects sperm protein expression presumably because of increasing testicular temperature. These proteins play essential roles in sperm production, DNA integrity protection, and sperm motility. This novel study demonstrates that varicocelectomy can improve both sperm quality and proteins expression.

Keywords

Varicocelectomy Sperm Protein Two dimensional gel electrophoresis 

Notes

Acknowledgments

We thank those patients who have participated in this study.

Conflict of interest

There is no conflict of interest.

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Hani Hosseinifar
    • 1
  • Marjan Sabbaghian
    • 1
  • Davood Nasrabadi
    • 3
  • Tahereh Modarresi
    • 1
  • Ahmad Vosough Taqi Dizaj
    • 4
  • Hamid Gourabi
    • 2
  • Mohmmad Ali Sadighi Gilani
    • 1
    • 5
  1. 1.Department of Andrology at Reproductive Biomedicine Research Center, Royan Institute for Reproductive BiomedicineACECRTehranIran
  2. 2.Department of Genetics at Reproductive Biomedicine Research Center, Royan Institute for Reproductive BiomedicineACECRTehranIran
  3. 3.Department of Molecular Systems Biology at Cell Science Research Center, Royan Institute for Stem Cell Biology and TechnologyACECRTehranIran
  4. 4.Department of Reproductive Imaging at Reproductive Biomedicine Research Center, Royan Institute for Reproductive BiomedicineACECRTehranIran
  5. 5.Department of UrologyShariati Hospital, Tehran University of Medical SciencesTehranIran

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