Study of the effect of varicocelectomy on sperm proteins expression in patients with varicocele and poor sperm quality by using two-dimensional gel electrophoresis
To compare proteomic profiles of spermatozoa from patients with varicocele and poor sperm quality before and after varicocelectomy.
This work was designed as a prospective and observational study. The study was based on 20 men with varicocele grade 3 and poor sperm quality undergoing varicocelectomy at the Fertility Unit of Royan institute in 2009. Two semen samples were collected, one before varicocelectomy and the other after surgery. Protein separation was done by two-dimensional protein electrophoresis, and analyzed by gel densitometry and mass spectrometry. Differential sperm protein expression levels were measured by gel densitometry.
Comparison of the sperm parameters showed that sperm motility and concentration were increased after varicocelectomy. At the level of protein, a total of 3 protein spots were identified whose expression was significantly lower in sperm samples before varicocelectomy compared with after surgery including heat shock protein A5 (HSPA5), superoxide dismutase 1 (SOD1) and δ-subunit of the catalytic core of mitochondrial adenosine triphosphate synthase (ATP5D).
High grade varicocoele affects sperm protein expression presumably because of increasing testicular temperature. These proteins play essential roles in sperm production, DNA integrity protection, and sperm motility. This novel study demonstrates that varicocelectomy can improve both sperm quality and proteins expression.
KeywordsVaricocelectomy Sperm Protein Two dimensional gel electrophoresis
We thank those patients who have participated in this study.
Conflict of interest
There is no conflict of interest.
- 5.de Mateo S, Martínez-Heredia J, Estanyol JM, Domínguez-Fandos D, Vidal-Taboada JM, Ballescà JL, et al. Marked correlations in protein expression identified by proteomic analysis of human spermatozoa. Proteomics. 2007;7:4264–77. Nature. 2003;422(6928):193–7. doi: 10.1038/nature01510.PubMedCrossRefGoogle Scholar
- 8.World Health Organization. WHO laboratory manual for the examination and processing of human semen. Cambridge: Cambridge University Press; 2010.Google Scholar
- 10.Neuhoff V, Arold N, Taube D, Ehrhardt W. Improved staining of proteins in polyacrylamide gels including isoelectric focusing gels with clear background at nanogram sensitivity using Coomassie Brilliant Blue G-250 and R-250. Electrophoresis. 1988;9(6):255–62. doi: 10.1002/elps.1150090603.PubMedCrossRefGoogle Scholar
- 12.Yesilli C, Mungan G, Seckiner I, Akduman B, Acikgoz S, Altan K, et al. Effect of varicocelectomy on sperm creatine kinase, HspA2 chaperone protein (creatine kinase-M type), LDH, LDH-X, and lipid peroxidation product levels in infertile men with varicocele. Urology. 2005;66(3):610–5. doi: 10.1016/j.urology.2005.03.078.PubMedCrossRefGoogle Scholar
- 22.Pixton KL, Deeks ED, Flesch FM, Moseley FL, Bjorndahl L, Ashton PR, et al. Sperm proteome mapping of a patient who experienced failed fertilization at IVF reveals altered expression of at least 20 proteins compared with fertile donors: case report. Hum Reprod. 2004;19(6):1438–47. doi: 10.1093/humrep/deh224deh224.PubMedCrossRefGoogle Scholar