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Effect of the site of assisted hatching on vitrified-warmed blastocyst transfer cycles: a prospective randomized study

  • ASSISTED REPRODUCTION TECHNOLOGIES
  • Published:
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Abstract

Purpose

To assess the effect of assisted hatching (AH) site on the clinical outcomes in vitrified-warmed blastocyst transfer cycles.

Methods

A total of 160 women who underwent vitrified-warmed blastocyst transfer cycles were randomized to either the ICM group (AH performing at the site near the inner cell mess, ICM), or the TE group (AH performing at the site opposite to the ICM). AH with laser zona drilling was performed 20 or 30 min after thawing once the ICM can be detected. Clinical pregnancy rate, implantation rate, live birth rate and the occurrence rate of monozygotic twins (MZT) pregnancy after transfer of these two groups were compared.

Results

No significant difference was found in the clinical pregnancy rate (63.8 % vs. 67.5 %), implantation rate (51.7 % vs. 53.6 %) and live birth rate (57.5 % vs. 62.5 %) between the ICM group and the TE group. The occurrence rate of MZT was comparable between the two groups (3.9 % vs. 5.6 %).

Conclusions

The site of assisted hatching has no influence on the implantation, pregnancy and live birth rate in human vitrified-warmed blastocyst transfer cycles.

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References

  1. Bleil JD, Wassarman PM. Structure and function of the zona pellucida: identification and characterization of the proteins of the mouse oocyte’s zona pellucida. Dev Biol. 1980;76:185–202.

    Article  PubMed  CAS  Google Scholar 

  2. van Duin M, Polman JE, De Breet IT, van Ginneken K, Bunschoten H, Grootenhuis A, et al. Recombinant human zona pellucida protein ZP3 produced by chinese hamster ovary cells induces the human sperm acrosome reaction and promotes sperm-egg fusion. Biol Reprod. 1994;51:607–17.

    Article  PubMed  Google Scholar 

  3. Modliński JA. The role of the zona pellucida in the development of mouse eggs in vivo. J Embryol Exp Morpholog. 1970;23:539–47.

    Google Scholar 

  4. Cohen J, Elsner C, Kort H, Malter H, Massey J, Mayer MP, et al. Impairment of the hatching process following IVF in the human and improvement of implantation by assisting hatching using micromanipulation. Hum Reprod. 1990;5:7–13.

    PubMed  CAS  Google Scholar 

  5. Cohen J, Alikani M, Trowbridge J, Rosenwaks Z. Implantation enhancement by selective assisted hatching using zona drilling of human embryos with poor prognosis. Hum Reprod. 1992;7:685–91.

    PubMed  CAS  Google Scholar 

  6. Das S, Blake D, Farquhar C, Seif MMW. Assisted hatching on assisted conception (IVF and ICSI). Cochrane Database Syst Rev. 2009;2, CD001894.

    PubMed  Google Scholar 

  7. Martins WP, Rocha IA, Ferriani RA, Nastri CO. Assisted hatching of human embryos: a systematic review and meta-analysis of randomized controlled trials. Hum Reprod Update. 2011;17:438–53.

    Article  PubMed  Google Scholar 

  8. Hammadeh ME, Fischer-Hammadeh C, Ali KR. Assisted hatching in assisted reproduction: a state of the art. J Assist Reprod Genet. 2011;28:119–28.

    Article  PubMed  Google Scholar 

  9. Lyu QF, Wu LQ, Li YP, Pan Q, Liu DE, Xia K, et al. An improved mechanical technique for assisted hatching. Hum Reprod. 2005;20:1619–23.

    Article  PubMed  CAS  Google Scholar 

  10. Hiraoka K, Fuchiwaki M, Hiraoka K, Horiuchi T, Murakami T, Kinutani M, et al. Effect of the size of zona pellucid opening by laser assisted hatching on clinical outcome of frozen cleaved embyos that were cultured to blastocyst after thawing in women with multiple implantation failures of embryo transfer: a retrospective study. J Assist Reprod Genet. 2008;25:129–35.

    Article  PubMed  Google Scholar 

  11. Hiraoka K, Hiraoka K, Horiuchi T, Kusuda T, Okano S, Kinutani M, et al. Impact of the size of zona pellucida thinning area on vitrified-warmed cleavage-stage embryo transfers: a prospective, randomized study. J Assist Reprod Genet. 2009;26:515–21.

    Article  PubMed  Google Scholar 

  12. Mantoudis E, Podsiadly BT, Gorgy A, Venkat G, Craft IL. A comparison between quarter, partial and total laser assisted hatching in selected infertility patients. Hum Reprod. 2001;16:2182–6.

    Article  PubMed  CAS  Google Scholar 

  13. Ng EH, Lau EY, Yeung WS, Cheung TM, Tang OS, Ho PC. Randomized double-blind comparison of laser zona pellucida thinning and breaching in frozen-thawed embryo transfer at the cleavage stage. Fertil Steril. 2008;89:1147–53.

    Article  PubMed  Google Scholar 

  14. Miyata H, Matsubayashi H, Fukutomi N, Matsuba J, Koizumi A, Tomiyama T. Relevance of the site of assisted hatching in thawed human blastocysts: a preliminary report. Fertil Steril. 2010;94:2444–7.

    Article  PubMed  Google Scholar 

  15. Ren X, Liu Q, Chen W, Zhu G, Li Y, Jin L, et al. Selection and vitrification of embryos with a poor morphological score: a proposal to avoid embryo wastage. J Huazhong Univ Sci Technol Med Sci. 2012;32:405–9.

    Article  PubMed  Google Scholar 

  16. Gardner DK, Lane M, Stevens J, Schlenker T, Schoolcraft WB. Blastocyst score affects implantation and pregnancy outcome: towards a single blastocyst transfer. Fertil Steril. 2000;73:1155–8.

    Article  PubMed  CAS  Google Scholar 

  17. Kuwayama M, Vajta G, Kato O, Leibo SP. Highly efficient vitrification method for cryopreservation of human oocytes. Reprod Biomed Online. 2005;11:300–8.

    Article  PubMed  Google Scholar 

  18. Mukaida T, Oka C, Goto T, Takahashi K. Artificial shrinkage of blastocoeles using either a micro-needle or a laser pulse prior to the cooling steps of vitrification improves survival rate and pregnancy outcome of vitrified human blastocysts. Hum Reprod. 2006;21:3246–52.

    Article  PubMed  CAS  Google Scholar 

  19. Gordon JW, Dapunt U. A new mouse model for embryos with a hatching deficiency and its use to elucidate the mechanism of blastocyst hatching. Fertil Steril. 1993;59:1296–301.

    PubMed  CAS  Google Scholar 

  20. Confino E, Rawlins R, Binor Z, Radwanska E. The effect of the oviduct, uterine, and in vitro environments on zona thinning in the mouse embryo. Fertil Steril. 1997;68:164–7.

    Article  PubMed  CAS  Google Scholar 

  21. Sawada H, Yamazaki K, Hoshi M. Trypsin-like hatching protease from mouse embryos: evidence for the presence in culture medium and its enzymatic properties. J Exp Zool. 1990;254:83–7.

    Article  PubMed  CAS  Google Scholar 

  22. Veeck LL, Zaninovic N. Blastocyst hatching. In: Veeck LL, Zaninovic N, editors. An atlas of human blastocysts. London: Informa Healthcare; 2003. p. 159–71.

    Google Scholar 

  23. Sathananthan H, Menezes J, Gunasheela S. Mechanics of human blastocyst hatching in vitro. Reprod Biomed Online. 2003;7:228–34.

    Article  PubMed  Google Scholar 

  24. Gonzales DS, Bavister BD. Zona pellucida escape by hamster blastocysts in vitro is delayed and morphologically different compared with zona escape in vivo. Biol Reprod. 1995;52:470–80.

    Article  PubMed  CAS  Google Scholar 

  25. Montag M, Koll B, Holmes P, van der Ven H. Significance of the number of embryonic cells and the state of the zona pellucida for hatching of mouse blastocysts in vitro versus in vivo. Biol Reprod. 2000;62:1738–44.

    Article  PubMed  CAS  Google Scholar 

  26. Lin SP, Lee RK, Tsai YJ. In vivo hatching phenomenon of mouse blastocysts during implantation. J Assist Reprod Genet. 2001;18:341–5.

    Article  PubMed  CAS  Google Scholar 

  27. Gonzales DS, Jones JM, Pinyopummintr T, Carnevale EM, Ginther OJ, Shapiro SS, et al. Trophectoderm projections: a potential means for locomotion, attachment and implantation of bovine equine and human blastocysts. Hum Reprod. 1996;11:2739–45.

    Article  PubMed  CAS  Google Scholar 

  28. Menezes J, Gunasheela S, Sathananthan H. Video observations on human blastocyst hatching. Reprod Biomed Online. 2003;7:217–8.

    Article  PubMed  Google Scholar 

  29. Ebner T, Vanderzwalmen P, Shebl O, Mayer RB, Moser M, Tews G. Morphological aspects of human blastocysts and the impact of vitrification. J Reproduktionsmed Endokrinol. 2011;8:13–20.

    Google Scholar 

  30. Ebner T, Gruber I, Moser M. Location of herniation predicts implantation behaviour of hatching blastocysts. J Turk Ger Gynecol Assoc. 2007;8:184–8.

    Google Scholar 

  31. Nikas G, Develioglu OH, Toner JP, Jones Jr HW. Endometrial pinopodes indicate a shift in the window of receptivity in IVF cycles. Hum Reprod. 1999;14:787–92.

    Article  PubMed  CAS  Google Scholar 

  32. Schieve LA, Meikle SF, Peterson HB, Jeng G, Burnett NM, Wilcox LS. Does assisted hatching pose a risk for monozygotic twinning in pregnancies conceived through in vitro fertilization? Fertil Steril. 2000;74:288–94.

    Article  PubMed  CAS  Google Scholar 

  33. Malter HE, Schimmel T, Cohen J. Zona dissection by infrared laser: developmental consequences in the mouse, technical considerations, and controlled clinical trial. Reprod Biomed Online. 2001;3:117–23.

    Article  PubMed  Google Scholar 

  34. Sun ST, Choi JR, Son JB, Joo JK, Ko GR, Lee KS. The effect of long zona dissection using ICSI pipettes for mechanical assisted hatching in vitrified-thawed blastocyst transfers. J Assist Reprod Genet. 2012;29:1431–4.

    Article  PubMed  Google Scholar 

  35. Hiraoka K, Fuchiwaki M, Hiraoka K, Horiuchi T, Murakami T, Kinutani M, et al. Zona pellucida removal and vitrified blastocyst transfer outcome: a preliminary study. Reprod Biomed Online. 2007;15:68–75.

    Article  PubMed  Google Scholar 

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Authors and Affiliations

  1. Reproductive Medicine Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1095 JieFang Avenue, Wuhan, 430030, People’s Republic of China

    Xinling Ren, Qun Liu, Wen Chen, Guijin Zhu & Hanwang Zhang

Authors
  1. Xinling Ren
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  2. Qun Liu
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  3. Wen Chen
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  4. Guijin Zhu
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  5. Hanwang Zhang
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Corresponding author

Correspondence to Hanwang Zhang.

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Capsule

The site of laser assisted hatching, at either the embryonic or abembryonic pole, has no influence on the pregnancy rate and live birth rate in vitrified-warmed blastocyst transfer cycles.

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Ren, X., Liu, Q., Chen, W. et al. Effect of the site of assisted hatching on vitrified-warmed blastocyst transfer cycles: a prospective randomized study. J Assist Reprod Genet 30, 691–697 (2013). https://doi.org/10.1007/s10815-013-9984-2

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  • DOI: https://doi.org/10.1007/s10815-013-9984-2

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