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High-magnification sperm selection does not decrease the aneuploidy rate in patients who are heterozygous for reciprocal translocations

  • Genetics
  • Published:
Journal of Assisted Reproduction and Genetics Aims and scope Submit manuscript

Abstract

Problem

This study sought to evaluate the value of motile sperm organelle morphology examination (MSOME) for selecting euploid spermatozoa in six patients who were heterozygous for a reciprocal translocation.

Method of study

We used sperm fluorescence in situ hybridization (FISH) to screen for aneuploidy of the chromosomes involved in the translocations and a putative interchromosomal effect (ICE) for chromosomes 18, X and Y. This procedure was performed on (i) whole sperm (i.e. no selection) and on normal spermatozoa selected (ii) at a magnification typically used for intracytoplasmic sperm injection (ICSI), referred to as “ICSI-like”, and (iii) with MSOME.

Results

The balanced translocation rates did not differ significantly (p = 0.81) when comparing whole sperm (57.2 %) with spermatozoa after ICSI-like selection (56.3 %) or after MSOME (53.7 %). Similarly, the aneuploidy rates for ICEs did not differ significantly (p = 0.14) when comparing whole sperm (1.9 %), ICSI-selected spermatozoa (3.4 %) and MSOME-selected spermatozoa (1.0 %).

Conclusion

For patients who are heterozygous for reciprocal translocations, MSOME does not improve the selection of euploid spermatozoa.

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Acknowledgment

This work was funded by the French Biomedicine Agency.

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Authors and Affiliations

  1. Department of Reproductive Biology, Cytogenetics, Gynaecology and Obstetrics, CHIPS, Centre Hospitalier Poissy Saint-Germain, 10 rue Champ-Gaillard, 78303, Poissy, France

    Mohamed Hassen Chelli, Fatma Ferfouri, Florence Boitrelle, Martine Albert, Denise Molina-Gomes, Jacqueline Selva & François Vialard

  2. EA 2493, University of Versailles Saint-Quentin en Yvelines (UVSQ), Versailles, France

    Fatma Ferfouri, Florence Boitrelle, Martine Albert, Jacqueline Selva & François Vialard

  3. Department of Reproductive Biology, Nouvelle Clinique du Parc, Tunis, Tunisia

    Mohamed Hassen Chelli

Authors
  1. Mohamed Hassen Chelli
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  2. Fatma Ferfouri
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  3. Florence Boitrelle
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  4. Martine Albert
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  5. Denise Molina-Gomes
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  6. Jacqueline Selva
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  7. François Vialard
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Corresponding author

Correspondence to François Vialard.

Additional information

Capsule High-magnification sperm selection nor ICSI selection do not decrease the aneuploidy rate in patients who are heterozygous for reciprocal translocations.

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Chelli, M.H., Ferfouri, F., Boitrelle, F. et al. High-magnification sperm selection does not decrease the aneuploidy rate in patients who are heterozygous for reciprocal translocations. J Assist Reprod Genet 30, 525–530 (2013). https://doi.org/10.1007/s10815-013-9959-3

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  • DOI: https://doi.org/10.1007/s10815-013-9959-3

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