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Expression of G protein estrogen receptor (GPER) on membrane of mouse oocytes during maturation

  • Gamete Biology
  • Published:
Journal of Assisted Reproduction and Genetics Aims and scope Submit manuscript

Abstract

Purpose

To determine expression of G-protein estrogen receptor (GPER) in mouse oocyte membrane during maturation.

Methods

The expression of GPER from different maturation stages of oocytes, in vivo and in vitro matured oocytes as well as aging oocytes was examined by immune-fluorescence GPR30 antibody and the images were analyzed by laser scanning confocal microscope. Further confirmation was performed by Western blots for cell fractionation.

Results

Significant fluorescent signal was observed on the surface of mouse oocytes. The image expression was lower in germinal vesicle (GV) stage than mature metaphase-II (M-II) stage oocytes. There was high expression in in-vivo matured oocytes compared to in vitro matured oocytes. The highest expression was observed in aging oocytes compared with other oocytes.

Conclusions

The changes of expression of GPER on mouse oocytes plasma membrane confirm oocyte membrane maturation, suggesting that those changes of GPER may be related to the functional role of oocyte maturation.

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Acknowledgments

This research was supported by MUHC Reproductive Center and a grant from the China Natural Science Foundation (81270746) to RCC.

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Authors and Affiliations

  1. Department of Obstetrics and Gynecology, Ningxia Medical University, Yinchuan, China

    Yi-Ran Li & Ji-Chun Li

  2. Department of Obstetrics and Gynecology, McGill University, Montreal, Canada

    Yi-Ran Li & Ri-Cheng Chian

  3. Department of Obstetrics and Gynecology, Weifang Medical Colleage, Weifang, China

    Chun-E Ren

  4. Center for Reproductive Medicine, Women and Infants Hospital of Qingdao, Qingdao, China

    Quan Zhang

  5. State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, China

    Ri-Cheng Chian

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  1. Yi-Ran Li
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  2. Chun-E Ren
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  3. Quan Zhang
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  4. Ji-Chun Li
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  5. Ri-Cheng Chian
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Correspondence to Ji-Chun Li or Ri-Cheng Chian.

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Capsule

The changes of expression of GPER on mouse oocytes plasma membrane confirm “oocyte membrane maturation”

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Li, YR., Ren, CE., Zhang, Q. et al. Expression of G protein estrogen receptor (GPER) on membrane of mouse oocytes during maturation. J Assist Reprod Genet 30, 227–232 (2013). https://doi.org/10.1007/s10815-013-9942-z

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  • DOI: https://doi.org/10.1007/s10815-013-9942-z

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