In vitro development of human primordial follicles to preantral stage after vitrification
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The aim was to culture primordial follicles in vitro to reach preantral stage in vitrified human ovarian tissue.
Ovarian tissue samples were obtained from six women. Tissue strips were vitrified by infiltration with a cryoprotectant followed by mounting on a stainless steel carrier. After culturing for 7 days the morphology and developmental stages of follicles enclosed in fresh and vitrified groups were analyzed.
High proportion of viable follicles in vitrified ovarian strips was obtained. After culturing for 7 days the percentage of secondary and preantral follicles increased significantly (P < 0.05) whereas primordial and transitory follicles showed a significant decrease (P < 0.05) compared to their respective counterparts at day 0 of culture.
Vitrification of ovarian strips with an improved carrier device and culturing of follicles in ovarian strips after warming yielded developed follicles with high viability and morphological integrity that may be suitable for use in fertility preservation among cancer patients.
KeywordsCarrier device In vitro culture Preantral follicles Preservation of fertility Primordial follicles
This research was supported, in part, by a grant from the Canadian Institutes of Health Research to F.W.K.K and by a research grant (No.17764) from the Tehran University of Medical Sciences to F.A.
- 17.Hashimoto S, Suzuki N, Amo A, Yamochi T, Hosoi Y, Morimoto Y. Good thermally conducting material supports follicle morphologies of porcine ovaries cryopreserved with ultrarapid vitrification. J Reprod Dev. 2013;in press.Google Scholar
- 22.Amorim CA, Dolmans MM, David A, Jaeger J, Vanacker J, Camboni A, et al. Vitrification and xenografting of human ovarian tissue. Fertil Steril. 2012;98:1291–8.Google Scholar
- 26.Li YB, Zhou CQ, Yang GF, Wang Q, Dong Y. Modified vitrification method for cryopreservation of human ovarian tissues. Chin Med J (Engl). 2007;120:110–4.Google Scholar
- 63.Salehnia M, Sheikhi M, Pourbeiranvand S, Lundqvist M. Apoptosis of human ovarian tissue is not increased by either vitrification or rapid cooling. Reprod Biomed Online. 2012;25:492–9.Google Scholar