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Journal of Assisted Reproduction and Genetics

, Volume 30, Issue 11, pp 1513–1518 | Cite as

The impact of semen quality, occupational exposure to environmental factors and lifestyle on recurrent pregnancy loss

  • Wang Ruixue
  • Zhou Hongli
  • Zhang Zhihong
  • Dai Rulin
  • Geng Dongfeng
  • Liu RuizhiEmail author
Gonadal Physiology and Disease

Abstract

Purpose

This study aimed to investigate the effect of male semen quality, occupational exposure, and lifestyle on recurrent pregnancy loss (RPL).

Methods

Information on couples’ occupational exposure and lifestyle was collected using a detailed questionnaire from 68 RPL couples and 63 randomly selected healthy controls. Semen parameters were estimated by computer-assisted sperm analysis, and sperm nuclear status was detected with aniline blue (AB) staining.

Results

Patients in the RPL group had significantly lower viability, normal morphology, and total progressive motility of sperm, and a higher mean percentage of AB staining positive sperm compared with those of controls (P < 0.05). There were no differences in sperm concentration, and motility between the groups (P > 0.05). Significant odds ratio (OR) was found when occupational exposure and unhealthy habits were superimposed (OR: 11.965, P = 0.005).

Conclusions

In addition to standard female factors for evaluating the risk for RPL, the use of male factors should also be taken into consideration. We found that sperm quality, occupational exposure, and lifestyle are factors that affect RPL. Consequently, occupational exposure and lifestyle factors should constitute an important section of questionnaires given to patients, and these factors should be evaluated by a clinician or trained staff.

Keywords

Male factors Recurrent pregnancy loss (RPL) Semen parameters Occupational exposure Lifestyle Semen quality 

Notes

Acknowledgments

This research was generously supported by a grant from National Population and Family Planning Commission of P.R.China (No.2011-GJKJS-07).

Conflicts of interest

The authors had no conflicts of interest to declare in relation to this article.

References

  1. 1.
    American Society for Reproductive Medicine. Practice Committee report: patient’s fact sheet: recurrent pregnancy loss. http://www.asrm.org/Patients/FactSheets/fact.html February 2005.
  2. 2.
    Allison JL, Schust DJ. Recurrent first trimester pregnancy loss: revised definitions and novel causes. Curr Opin Endocrinol Diabetes Obes. 2009;16(6):446–50.PubMedCrossRefGoogle Scholar
  3. 3.
    Kiwi R. Recurrent pregnancy loss: evaluation and discussion of the causes and their management. Cleve Clin J Med. 2006;73(10):913–21.PubMedCrossRefGoogle Scholar
  4. 4.
    Puscheck EE, Jeyendran RS. The impact of male factor on recurrent pregnancy loss. Curr Opin Obstet Gynecol. 2007;19(3):222–8.PubMedCrossRefGoogle Scholar
  5. 5.
    Barroso G, Valdespin C, Vega E, et al. Developmental sperm contributions: fertilization and beyond. Fertil Steril. 2009;92(3):835–48.PubMedCrossRefGoogle Scholar
  6. 6.
    Hill JA, Abbott AF, Politch JA. Sperm morphology and recurrent abortion. Fertil Steril. 1994;61(4):776–8.PubMedGoogle Scholar
  7. 7.
    Talebi AR, Vahidi S, Aflatoonian A, et al. Cytochemical evaluation of sperm chromatin and DNA integrity in couples with unexplained recurrent spontaneous abortions. Andrologia. 2012;44 Suppl 1:462–70.PubMedCrossRefGoogle Scholar
  8. 8.
    Absalan F, Ghannadi A, Kazerooni M, et al. Value of sperm chromatin dispersion test in couples with unexplained recurrent abortion. J Assist Reprod Genet. 2012;29(1):11–4.PubMedCrossRefGoogle Scholar
  9. 9.
    Venkatesh S, Thilagavathi J, Kumar K, et al. Cytogenetic, Y chromosome microdeletion, sperm chromatin and oxidative stress analysis in male partners of couples experiencing recurrent spontaneous abortions. Arch Gynecol Obstet. 2011;284(6):1577–84.PubMedCrossRefGoogle Scholar
  10. 10.
    Miranda-Contreras L, Gómez-Pérez R, Rojas G, et al. (2013) Occupational exposure to organophosphate and carbamate pesticides affects sperm chromatin integrity and reproductive hormone levels among Venezuelan farm workers. J Occup Health. doi: 10.1539/joh.12-0144-FS.
  11. 11.
    Martenies SE, Perry MJ. Environmental and occupational pesticide exposure and human sperm parameters: a systematic review. Toxicology. 2013;307(10):66–73.PubMedCrossRefGoogle Scholar
  12. 12.
    Gardella JR, Hill JA. Environmental toxins associated with recurrent pregnancy loss. Semin Reprod Med. 2000;18(4):407–24.PubMedCrossRefGoogle Scholar
  13. 13.
    Sharara FI, Seifer DB, Flaws JA. Environmental toxicants and female reproduction. Fertil Steril. 1998;70(4):613–22.PubMedCrossRefGoogle Scholar
  14. 14.
    World Health Organization. Laboratory manual for the examination of human semen and sperm–cervical mucus interaction. 4th ed. Cambridge: Cambridge University Press; 1999.Google Scholar
  15. 15.
    Nasr-Esfahani MH, Razavi S, Mardani M. Relation between different human sperm nuclear maturity tests and in vitro fertilization. J Assist Reprod Genet. 2001;18(4):219–25.PubMedCrossRefGoogle Scholar
  16. 16.
    Brahem S, Mehdi M, Landolsi H, et al. Semen parameters and sperm DNA fragmentation as causes of recurrent pregnancy loss. Urology. 2011;78(4):792–6.PubMedCrossRefGoogle Scholar
  17. 17.
    Gil-Villa AM, Cardona-Maya W, Agarwal A, et al. Assessment of sperm factors possibly involved in early recurrent pregnancy loss. Fertil Steril. 2010;94(4):1465–72.PubMedCrossRefGoogle Scholar
  18. 18.
    Zhang L, Wang L, Zhang X, et al. Sperm chromatin integrity may predict future fertility for unexplained recurrent spontaneous abortion patients. Int J Androl. 2012;35(5):752–7.PubMedCrossRefGoogle Scholar
  19. 19.
    Saxena P, Misro MM, Chaki SP, et al. Is abnormal sperm function an indicator among couples with recurrent pregnancy loss? Fertil Steril. 2008;90(5):1854–8.PubMedCrossRefGoogle Scholar
  20. 20.
    Robinson L, Gallos ID, Conner SJ, et al. The effect of sperm DNA fragmentation on miscarriage rates: a systematic review and meta-analysis. Hum Reprod. 2012;27(10):2908–17.PubMedCrossRefGoogle Scholar
  21. 21.
    Agarwal A, Sharma RK, Nelson DR. New semen quality scores developed by principal component analysis of semen characteristics. J Androl. 2003;24(3):343–52.PubMedGoogle Scholar
  22. 22.
    Allamaneni SS, Bandaranayake I, Agarwal A. Use of semen quality scores to predict pregnancy rates in couples undergoing intrauterine insemination with donor sperm. Fertil Steril. 2004;82(3):606–11.PubMedCrossRefGoogle Scholar
  23. 23.
    De Fleurian G, Perrin J, Ecochard R, et al. Occupational exposures obtained by questionnaire in clinical practice and their association with semen quality. J Androl. 2009;30(5):566–79.PubMedCrossRefGoogle Scholar
  24. 24.
    Braga DP, Halpern G, Figueira Rde C. Food intake and social habits in male patients and its relationship to intracytoplasmic sperm injection outcomes. Fertil Steril. 2012;97(1):53–9.PubMedCrossRefGoogle Scholar
  25. 25.
    Zitzmann M, Rolf C, Nordhoff V, et al. Male smokers have a decreased success rate for in vitro fertilization and intracytoplasmic sperm injection. Fertil Steril. 2003;79 Suppl 3:1550–4.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Wang Ruixue
    • 1
  • Zhou Hongli
    • 1
  • Zhang Zhihong
    • 1
  • Dai Rulin
    • 1
  • Geng Dongfeng
    • 1
  • Liu Ruizhi
    • 1
    • 2
    Email author
  1. 1.Center for Reproductive Medicine, The First HospitalJilin UniversityChangchunChina
  2. 2.The First Hospital of Jilin UniversityChangchunChina

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