Journal of Assisted Reproduction and Genetics

, Volume 30, Issue 11, pp 1421–1429 | Cite as

Serum markers of ovarian reserve and ovarian histology in adult mice treated with cyclophosphamide in pre-pubertal age

  • Laura Detti
  • Rebecca A. Uhlmann
  • Meifen Lu
  • Michael P. Diamond
  • Ghassan M. Saed
  • Nicole M. Fletcher
  • Jie Zhang
  • Lucy J. Williams
Fertility Preservation



AMH is used to quantify the extent of follicular pool in postpubertal women, but its value after chemotherapy is unclear. We tested AMH as a marker of follicular reserve in adult mice treated with cyclophosphamide (CTX) in prepubertal age.


Mice received placebo or CTX at age 18 days. AMH and FSH were assessed on day 43, 56, and 95 of life. Ovaries were fixed in formalin, embedded in paraffin, and stained with H&E and TUNEL. Follicular apoptosis was graded.


All mice exposed to CTX had a decreased number of follicles/mm2 and significantly decreased AMH, but only 48 % of pubertal and 81 % of adult mice had increased FSH. Over time, there was an increase in FSH (p < 0.05), but not a concurrent decrease in AMH, while in controls, FSH remained stable and AMH decreased. There was no correlation between histological and serological markers.


CTX administration to pre-pubertal mice caused various degrees of residual function, which were reflected by FSH, but not by AMH or by the number of ovarian follicles. AMH served as a marker of quantitative, and FSH of qualitative, residual ovarian function.


Animal study Prepubertal Cyclophosphamide AMH FSH Follicle count 



The study was funded with an institutional grant from the University of Tennessee Health Science Center to Dr. Laura Detti.


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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Laura Detti
    • 1
  • Rebecca A. Uhlmann
    • 1
  • Meifen Lu
    • 2
  • Michael P. Diamond
    • 3
  • Ghassan M. Saed
    • 4
  • Nicole M. Fletcher
    • 4
  • Jie Zhang
    • 2
  • Lucy J. Williams
    • 1
  1. 1.Department of Obstetrics and GynecologyUniversity of Tennessee Health Science CenterMemphisUSA
  2. 2.Department of PathologyMemphisUSA
  3. 3.Georgia Regents UniversityDepartment of Obstetrics and GynecologyAugustaUSA
  4. 4.Department of Obstetrics and GynecologyWayne State UniversityDetroitUSA

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