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Serum markers of ovarian reserve and ovarian histology in adult mice treated with cyclophosphamide in pre-pubertal age

  • Fertility Preservation
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Abstract

Purpose

AMH is used to quantify the extent of follicular pool in postpubertal women, but its value after chemotherapy is unclear. We tested AMH as a marker of follicular reserve in adult mice treated with cyclophosphamide (CTX) in prepubertal age.

Methods

Mice received placebo or CTX at age 18 days. AMH and FSH were assessed on day 43, 56, and 95 of life. Ovaries were fixed in formalin, embedded in paraffin, and stained with H&E and TUNEL. Follicular apoptosis was graded.

Results

All mice exposed to CTX had a decreased number of follicles/mm2 and significantly decreased AMH, but only 48 % of pubertal and 81 % of adult mice had increased FSH. Over time, there was an increase in FSH (p < 0.05), but not a concurrent decrease in AMH, while in controls, FSH remained stable and AMH decreased. There was no correlation between histological and serological markers.

Conclusions

CTX administration to pre-pubertal mice caused various degrees of residual function, which were reflected by FSH, but not by AMH or by the number of ovarian follicles. AMH served as a marker of quantitative, and FSH of qualitative, residual ovarian function.

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Acknowledgments

The study was funded with an institutional grant from the University of Tennessee Health Science Center to Dr. Laura Detti.

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Authors and Affiliations

  1. Department of Obstetrics and Gynecology, University of Tennessee Health Science Center, Rout Center, 853 Jefferson Ave, Room E102, Memphis, TN, 38163, USA

    Laura Detti, Rebecca A. Uhlmann & Lucy J. Williams

  2. Department of Pathology, Memphis, TN, USA

    Meifen Lu & Jie Zhang

  3. Georgia Regents University, Department of Obstetrics and Gynecology, Augusta, GA, USA

    Michael P. Diamond

  4. Department of Obstetrics and Gynecology, Wayne State University, Detroit, MI, USA

    Ghassan M. Saed & Nicole M. Fletcher

Authors
  1. Laura Detti
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  2. Rebecca A. Uhlmann
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  3. Meifen Lu
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  4. Michael P. Diamond
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  5. Ghassan M. Saed
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  6. Nicole M. Fletcher
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  7. Jie Zhang
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  8. Lucy J. Williams
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Corresponding author

Correspondence to Laura Detti.

Additional information

Capsule AMH is a marker of quantitative, and FSH of qualitative, residual ovarian function after cyclophosphamide administration to pre-pubertal mice.

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Detti, L., Uhlmann, R.A., Lu, M. et al. Serum markers of ovarian reserve and ovarian histology in adult mice treated with cyclophosphamide in pre-pubertal age. J Assist Reprod Genet 30, 1421–1429 (2013). https://doi.org/10.1007/s10815-013-0087-x

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  • DOI: https://doi.org/10.1007/s10815-013-0087-x

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