Journal of Assisted Reproduction and Genetics

, Volume 30, Issue 11, pp 1415–1419 | Cite as

Case report: the use of annexin V coupled with magnetic activated cell sorting in cryopreserved spermatozoa from a male cancer survivor: healthy twin newborns after two previous ICSI failures

  • María Belén Herrero
  • Géraldine Delbes
  • Jin-Tae Chung
  • Weon-Young Son
  • Hananel Holzer
  • William Buckett
  • Peter ChanEmail author
Fertility Preservation



The aim of the study is to report successful outcome (live births) after sperm sorting with annexin V-MACS on cryopreserved spermatozoa with high level of sperm DNA fragmentation from a cancer patient survivor.


Cryopreserved spermatozoa were sorted with annexin V-MACS prior to ICSI. Sperm DNA fragmentation was evaluated by SCSA® and TUNEL.


The couple had two previous IVF/ICSI cycles failures using sperm cryopreserved before cancer treatment. On third ICSI cycle attempt results were as follow: pre-annexin V-MACS sperm quality: 10 × 106/ml, 3.3 % progressive motility, 1 % normal forms, TUNEL: 72.5 % positive cells, SCSA®: 76.6 % DFI. Post-annexin V-MACS sperm quality: 2.8 × 106/ml, 10 % progressive motility, TUNEL: 58.8 % positive cells. Eight metaphase II oocytes were collected, 4 fertilized, 2 embryos were transferred on day 3 and healthy twins were born (1 boy, 1 girl).


Annexin V-MACS technique could be a potential tool to improve sperm quality on cryopreserved spermatozoa of cancer patient and improve ICSI outcome.


Cancer survivor Cryopreserved sperm Annexin V-MACS 



The authors wish to thank Lorraine Lavigne, R.N., for co-ordination of all clinical visits with the couple and to the Centro de Estudios en Ginecología y Reproducción (CEGYR, Argentina), for sharing their knowledge on MACS columns. The study was supported by CIHR grants SUR-103395, MOP-86636 to PC.


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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • María Belén Herrero
    • 1
  • Géraldine Delbes
    • 2
  • Jin-Tae Chung
    • 1
  • Weon-Young Son
    • 1
  • Hananel Holzer
    • 1
  • William Buckett
    • 1
  • Peter Chan
    • 1
    • 2
    • 3
    Email author
  1. 1.MUHC Reproductive CenterMcGill University Health CenterMontrealCanada
  2. 2.The Urology Research DepartmentMcGill University Health CenterMontrealCanada
  3. 3.The Department of UrologyMcGill University Health Center Royal Victoria HospitalMontréalCanada

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