Journal of Assisted Reproduction and Genetics

, Volume 30, Issue 9, pp 1227–1230 | Cite as

Retrieval and in vitro maturation of human oocytes from ovaries removed during surgery for endometrial carcinoma: a novel strategy for human oocyte research

  • Hiromitsu Shirasawa
  • Jin Kumagai
  • Wataru Sato
  • Yukiyo Kumazawa
  • Naoki Sato
  • Yukihiro Terada
Gamete Biology



To collect human oocytes from ovaries removed as part of surgical treatment for endometrial carcinoma, and to induce in vitro maturation of such oocytes to obtain material for research on human ovarian aging.


Design: Prospective clinical study. Setting: University Hospital. Patients: Eight patients aged 35–44 years with a preoperative diagnosis of Stage I endometrial cancer agreed to participate in this project. Interventions: Surgically removed ovaries were punctured; oocytes were collected from follicular fluid and matured in vitro. Immunofluorescent detection of microtubules and DNA labeling were performed after in vitro maturation. Main Outcome Measures: Number of oocytes collected and their in vitro maturation stage.


In total, 87 oocytes were collected, 11 of which had completed metaphase II. Of the oocytes collected, 75 % were from three patients in their 30s, while the remaining 25 % were from five patients in their 40s. Several stages of oocytes were collected and the detection of microtubule arrangement and chromatin in various stages using fluorescence was possible.


Material for research on human ovarian aging can be obtained from ovaries removed during surgery for endometrial cancer.


Human oocytes In vitro maturation Microtubules 



Financial support was provided by the Japan Society for the Promotion of Science (Y.T.) and the Uehara Memorial Fundation (Y.T.).


  1. 1.
    Battaglia DE, Goodwin P, Klein NA, Soules MR. Influence of maternal age on meiotic spindle assembly in oocytes from naturally cycling women. Hum Reprod (Oxford, England). 1996;11(10):2217–22.CrossRefGoogle Scholar
  2. 2.
    Chiang T, Duncan FE, Schindler K, Schultz RM, Lampson MA. Evidence that weakened centromere cohesion is a leading cause of age-related aneuploidy in oocytes. Curr Biol: CB. 2010;20(17):1522–8. doi: 10.1016/j.cub.2010.06.069.PubMedCrossRefGoogle Scholar
  3. 3.
    Hasegawa H. The best approach to observe microtubule organization in oocyte cytoplasm production of oocyte wole mount samples by Buffer M Fixation. J Mamm Ova Res. 2009;26(4):232–3. doi: 10.1274/jmor.26.232.CrossRefGoogle Scholar
  4. 4.
    Hassold T, Hunt P. To err (meiotically) is human: the genesis of human aneuploidy. Nat Rev Genet. 2001;2(4):280–91. doi: 10.1038/35066065.PubMedCrossRefGoogle Scholar
  5. 5.
    Humphrey MM, Apte SM. The use of minimally invasive surgery for endometrial cancer. Cancer Control : J Moffitt Cancer Center. 2009;16(1):30–7.Google Scholar
  6. 6.
    Krey LC, Grifo JA. Poor embryo quality: the answer lies (mostly) in the egg. Fertil Steril. 2001;75(3):466–8.PubMedCrossRefGoogle Scholar
  7. 7.
    Lim AS, Tsakok MF. Age-related decline in fertility: a link to degenerative oocytes? Fertil Steril. 1997;68(2):265–71.PubMedCrossRefGoogle Scholar
  8. 8.
    Lister LM, Kouznetsova A, Hyslop LA, Kalleas D, Pace SL, Barel JC, et al. Age-related meiotic segregation errors in mammalian oocytes are preceded by depletion of cohesin and Sgo2. Curr Biol: CB. 2010;20(17):1511–21. doi: 10.1016/j.cub.2010.08.023.PubMedCrossRefGoogle Scholar
  9. 9.
    McLaren A. Free-range eggs? Science (New York, NY). 2007;316(5823):339. doi: 10.1126/science.1142267.CrossRefGoogle Scholar
  10. 10.
    Morita J, Terada Y, Hosoi Y, Fujinami N, Sugimoto M, Nakamura S-I, et al. Microtubule organization during rabbit fertilization by intracytoplasmic sperm injection with and without sperm centrosome. Reprod Med Biol. 2005;4(2):169–78. doi: 10.1111/j.1447-0578.2005.00096.x.CrossRefGoogle Scholar
  11. 11.
    Revel A, Safran A, Benshushan A, Shushan A, Laufer N, Simon A. In vitro maturation and fertilization of oocytes from an intact ovary of a surgically treated patient with endometrial carcinoma: case report. Hum Reprod (Oxford, England). 2004;19(7):1608–11. doi: 10.1093/humrep/deh241.CrossRefGoogle Scholar
  12. 12.
    Selesniemi K, Lee HJ, Muhlhauser A, Tilly JL. Prevention of maternal aging-associated oocyte aneuploidy and meiotic spindle defects in mice by dietary and genetic strategies. Proc Natl Acad Sci U S A. 2011;108(30):12319–24. doi: 10.1073/pnas.1018793108.PubMedCrossRefGoogle Scholar
  13. 13.
    Subramanian VV, Bickel SE. Aging predisposes oocytes to meiotic nondisjunction when the cohesin subunit SMC1 is reduced. PLoS Genet. 2008;4(11):e1000263. doi: 10.1371/journal.pgen.1000263.PubMedCrossRefGoogle Scholar
  14. 14.
    Vogt E, Kirsch-Volders M, Parry J, Eichenlaub-Ritter U. Spindle formation, chromosome segregation and the spindle checkpoint in mammalian oocytes and susceptibility to meiotic error. Mutat Res. 2008;651(1–2):14–29. doi: 10.1016/j.mrgentox.2007.10.015.PubMedGoogle Scholar
  15. 15.
    Wiser A, Son WY, Shalom-Paz E, Reinblatt SL, Tulandi T, Holzer H. How old is too old for in vitro maturation (IVM) treatment? Eur J Obstet Gynecol Reprod Biol. 2011;159(2):381–3. doi: 10.1016/j.ejogrb.2011.09.009.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Hiromitsu Shirasawa
    • 1
  • Jin Kumagai
    • 1
  • Wataru Sato
    • 1
  • Yukiyo Kumazawa
    • 1
  • Naoki Sato
    • 1
  • Yukihiro Terada
    • 1
    • 2
  1. 1.Department of Obstetrics and GynecologyAkita University Graduate School of MedicineAkitaJapan
  2. 2.Department of Obstetrics and GynecologyTohoku University Graduate School of MedicineSendai MiyagiJapan

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