Abstract
Purpose
To collect human oocytes from ovaries removed as part of surgical treatment for endometrial carcinoma, and to induce in vitro maturation of such oocytes to obtain material for research on human ovarian aging.
Methods
Design: Prospective clinical study. Setting: University Hospital. Patients: Eight patients aged 35–44 years with a preoperative diagnosis of Stage I endometrial cancer agreed to participate in this project. Interventions: Surgically removed ovaries were punctured; oocytes were collected from follicular fluid and matured in vitro. Immunofluorescent detection of microtubules and DNA labeling were performed after in vitro maturation. Main Outcome Measures: Number of oocytes collected and their in vitro maturation stage.
Results
In total, 87 oocytes were collected, 11 of which had completed metaphase II. Of the oocytes collected, 75 % were from three patients in their 30s, while the remaining 25 % were from five patients in their 40s. Several stages of oocytes were collected and the detection of microtubule arrangement and chromatin in various stages using fluorescence was possible.
Conclusion
Material for research on human ovarian aging can be obtained from ovaries removed during surgery for endometrial cancer.
Similar content being viewed by others
References
Battaglia DE, Goodwin P, Klein NA, Soules MR. Influence of maternal age on meiotic spindle assembly in oocytes from naturally cycling women. Hum Reprod (Oxford, England). 1996;11(10):2217–22.
Chiang T, Duncan FE, Schindler K, Schultz RM, Lampson MA. Evidence that weakened centromere cohesion is a leading cause of age-related aneuploidy in oocytes. Curr Biol: CB. 2010;20(17):1522–8. doi:10.1016/j.cub.2010.06.069.
Hasegawa H. The best approach to observe microtubule organization in oocyte cytoplasm production of oocyte wole mount samples by Buffer M Fixation. J Mamm Ova Res. 2009;26(4):232–3. doi:10.1274/jmor.26.232.
Hassold T, Hunt P. To err (meiotically) is human: the genesis of human aneuploidy. Nat Rev Genet. 2001;2(4):280–91. doi:10.1038/35066065.
Humphrey MM, Apte SM. The use of minimally invasive surgery for endometrial cancer. Cancer Control : J Moffitt Cancer Center. 2009;16(1):30–7.
Krey LC, Grifo JA. Poor embryo quality: the answer lies (mostly) in the egg. Fertil Steril. 2001;75(3):466–8.
Lim AS, Tsakok MF. Age-related decline in fertility: a link to degenerative oocytes? Fertil Steril. 1997;68(2):265–71.
Lister LM, Kouznetsova A, Hyslop LA, Kalleas D, Pace SL, Barel JC, et al. Age-related meiotic segregation errors in mammalian oocytes are preceded by depletion of cohesin and Sgo2. Curr Biol: CB. 2010;20(17):1511–21. doi:10.1016/j.cub.2010.08.023.
McLaren A. Free-range eggs? Science (New York, NY). 2007;316(5823):339. doi:10.1126/science.1142267.
Morita J, Terada Y, Hosoi Y, Fujinami N, Sugimoto M, Nakamura S-I, et al. Microtubule organization during rabbit fertilization by intracytoplasmic sperm injection with and without sperm centrosome. Reprod Med Biol. 2005;4(2):169–78. doi:10.1111/j.1447-0578.2005.00096.x.
Revel A, Safran A, Benshushan A, Shushan A, Laufer N, Simon A. In vitro maturation and fertilization of oocytes from an intact ovary of a surgically treated patient with endometrial carcinoma: case report. Hum Reprod (Oxford, England). 2004;19(7):1608–11. doi:10.1093/humrep/deh241.
Selesniemi K, Lee HJ, Muhlhauser A, Tilly JL. Prevention of maternal aging-associated oocyte aneuploidy and meiotic spindle defects in mice by dietary and genetic strategies. Proc Natl Acad Sci U S A. 2011;108(30):12319–24. doi:10.1073/pnas.1018793108.
Subramanian VV, Bickel SE. Aging predisposes oocytes to meiotic nondisjunction when the cohesin subunit SMC1 is reduced. PLoS Genet. 2008;4(11):e1000263. doi:10.1371/journal.pgen.1000263.
Vogt E, Kirsch-Volders M, Parry J, Eichenlaub-Ritter U. Spindle formation, chromosome segregation and the spindle checkpoint in mammalian oocytes and susceptibility to meiotic error. Mutat Res. 2008;651(1–2):14–29. doi:10.1016/j.mrgentox.2007.10.015.
Wiser A, Son WY, Shalom-Paz E, Reinblatt SL, Tulandi T, Holzer H. How old is too old for in vitro maturation (IVM) treatment? Eur J Obstet Gynecol Reprod Biol. 2011;159(2):381–3. doi:10.1016/j.ejogrb.2011.09.009.
Acknowledgments
Financial support was provided by the Japan Society for the Promotion of Science (Y.T.) and the Uehara Memorial Fundation (Y.T.).
Author information
Authors and Affiliations
Corresponding author
Additional information
Capsule Surgically removed ovaries from patients with endometrial carcinoma represent a new source of material for human oocyte research.
Rights and permissions
About this article
Cite this article
Shirasawa, H., Kumagai, J., Sato, W. et al. Retrieval and in vitro maturation of human oocytes from ovaries removed during surgery for endometrial carcinoma: a novel strategy for human oocyte research. J Assist Reprod Genet 30, 1227–1230 (2013). https://doi.org/10.1007/s10815-013-0040-z
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10815-013-0040-z