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The influence of amifostine administration prior to cyclophosphamide on in vitro maturation of mouse oocytes

  • Gamete Biology
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Abstract

Purpose

The protective effect of amifostine against cyclophosphamide (CP) was evaluated on mouse oocytes.

Materials and methods

Female mice were divided into four groups as follows: group1: cyclophosphamide (CP) (75 mg/kg, i.p) injection, group2: amifostine (250 mg/kg, i.p) injection, group3: amifostine (250 mg/kg, i.p) administered prior to CP (75 mg/kg, i.p) injection, Control group with injection of saline. About 21 days after injection, in vitro maturation (IVM) of oocytes was recorded. Furthermore the percentage of aneuploid oocytes was determined.

Results

In the CP group IVM rate was significantly decreased and aneploidy rate was significantly increased when compared to other groups (p < 0.05). With the administration of Amifostine prior to CP injection IVM rate was increased and aneploidy rate was decreased.

Discussion

Depletion in IVM rate with administration of CP is due its adverse effects on oocyte quality. Amifostine administration prior to CP injection appears to modulate deleterious effects of CP on oocytes.

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Acknowledgments

This research supported by Royan Institute.

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Correspondence to Hamid Gourabi.

Additional information

Capsule Amifostine administration prior to Cyclophosphamide protects ovarian oocytes and it may provides fertility preservation in cancer patients.

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Golkar-Narenji, A., Barekati, Z., Eimani, H. et al. The influence of amifostine administration prior to cyclophosphamide on in vitro maturation of mouse oocytes. J Assist Reprod Genet 30, 939–944 (2013). https://doi.org/10.1007/s10815-013-0035-9

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  • DOI: https://doi.org/10.1007/s10815-013-0035-9

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