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Do ACE I/D gene polymorphism serve as a predictive marker for age at onset in PCOS?

  • Genetics
  • Published:
Journal of Assisted Reproduction and Genetics Aims and scope Submit manuscript

Abstract

Background

Polycystic ovary syndrome (PCOS) is an endocrine disorder exhibiting variable age at onset of clinical features allied with complex diseases in the later life. ACE is a pleiotropic molecule associated with various pathophysiological functions. The present study was aimed to establish the frequency of ACE I/D gene polymorphism in patients and controls and to assess the influence of this polymorphism on anthropometric and various clinical features of the condition.

Methods

ACE I/D genotyping was carried out in 259 PCOS patients and 315 healthy ultrasound scanned women of South Indian origin.

Results

The distribution of DD, ID and II genotypes in patients was 39, 37 and 24 %, whereas in the controls it was 31, 51 and 18 % respectively. Significant difference was observed in the genotypic frequency distributions between the patients and controls, however the allelic frequencies did not vary between the groups (p > 0.05). Quartile analysis revealed preponderance of DD genotype in the first two quartiles and a linear increase of II genotype from first to the last quartiles. Further, Multiple Logistic regression analysis revealed significant association of ACE I/D gene polymorphism with acanthosis and age at onset (AAO) of the syndrome (p < 0.05).

Conclusion

The present study is the first report to highlight the predisposing role of DD and protective role of ID genotype towards PCOS. Patients with single or double dose of D allele may develop PCOS symptoms at an early age and also significantly associated with acanthosis, a marker of insulin resistance.

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Abbreviations

PCOS:

Polycystic Ovary Syndrome

ACE:

Angiotensin converting enzyme

AAO:

Age at onset

BMI:

Body mass index

W/R:

Waist and Hip ratio

OR:

Odds ratio

RAS:

Renin Angiotensin System

I/D:

insertion/deletion

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Acknowledgments

We thank all the subjects for their co-operation in giving consent for blood sample and the clinical information. And we would also like to thank all the doctors and nursing staff of various Endocrinological Clinics of Hyderabad, (India) for supporting the implementation of the study and assisting with data collection. We further thank Indian Council of Medical Research (ICMR), India for providing financial assistance to M.L.N Deepika.

Disclosure statement

The authors declare that no competing financial interests exist.

Author’s contribution

Deepika M.L.N performed the experiment and drafted the manuscript; K. Ranjith worked on initial analysis of data generated, Dr. Usha Rani helped in sampling, Dr. N Balakrishna carried out the statistical analysis, Dr. Parveen Jahan and K. Prasanna Latha contributed to the preparation of manuscript.

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Authors and Affiliations

  1. Department of Genetics, Osmania University, Hyderabad, 500 007, Andhra Pradesh, India

    M. L. N. Deepika, K. Ranjith Reddy, K. Prasanna Latha & Parveen Jahan

  2. Department of Obstetrics and Gynecology, Modern Government Maternity Hospital, Petlaburz, Hyderabad, India

    V. Usha Rani

  3. Department of Biostatistics, National Institute of Nutrition, Tarnaka, Hyderabad, India

    N. Balakrishna

Authors
  1. M. L. N. Deepika
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  2. K. Ranjith Reddy
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  3. V. Usha Rani
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  4. N. Balakrishna
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  5. K. Prasanna Latha
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  6. Parveen Jahan
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Corresponding author

Correspondence to Parveen Jahan.

Additional information

Capsule

The ACE ID genotype confer protection to PCOS in our population. The D allele either in single or double dose appears to influence the age at onset of the disease.

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Deepika, M.L.N., Reddy, K.R., Rani, V.U. et al. Do ACE I/D gene polymorphism serve as a predictive marker for age at onset in PCOS?. J Assist Reprod Genet 30, 125–130 (2013). https://doi.org/10.1007/s10815-012-9906-8

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  • DOI: https://doi.org/10.1007/s10815-012-9906-8

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