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CASA derived human sperm abnormalities: correlation with chromatin packing and DNA fragmentation

  • Gamete Biology
  • Published:
Journal of Assisted Reproduction and Genetics Aims and scope Submit manuscript

Abstract

Purpose

The present study was undertaken to evaluate the effects of morphokinetic abnormalities of human spermatozoa on chromatin packing and DNA integrity and possible beneficial effects of sperm selection in ICSI.

Methods

Semen samples from 1002 patients were analysed for morphology and motility using CASA. Protamine status and DNA fragmentation were analysed by chromomycin A3 staining and sperm chromatin dispersion assay respectively.

Results

Sperms with elongated, thin, round, pyri, amorphous, micro and macro forms were significantly higher in teratozoospermic and oligoasthenoteratozoospermic groups. Significant difference in chromatin packing and DNA fragmentation index was observed in these abnormal groups compared with normal. Similarly significant correlation was also seen between abnormal motility parameters and DNA fragmentation index in asthenozoospermic group compared with normal.

Conclusions

Specific abnormal morphological forms have higher incidence of chromatin packing abnormalities and DNA fragmentation. Using these sperms in ICSI might have an impact on fertilization, embryo development and abortion rates. These can be selectively avoided during ICSI procedure to improve ART outcome.

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Acknowledgement

The authors thank andrology lab team Mr. Ramana, Ramesh and Mrs. Mythili for technical help.

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Authors and Affiliations

  1. Gynecology Division, Krishna IVF Clinic, Krishna Nagar, Visakhapatnam, 530 002, Andhra Pradesh, India

    B. Sireesha Rani & G. A. Rama Raju

  2. Embryology Research Group, Krishna IVF Clinic, Krishna Nagar, Visakhapatnam, 530 002, Andhra Pradesh, India

    G. Jaya Prakash, K. Murali Krishna & K. Madan

  3. Genetics Department, Krishna IVF Clinic, Krishna Nagar, Visakhapatnam, 530 002, Andhra Pradesh, India

    T. Sivanarayana & Ch. Ravi Krishna

  4. Department of Human Genetics, Andhra University, Visakhapatnam, 530 003, Andhra Pradesh, India

    T. Sivanarayana, Ch. Ravi Krishna & G. Sudhakar

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  1. T. Sivanarayana
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  2. Ch. Ravi Krishna
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  3. G. Jaya Prakash
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  4. K. Murali Krishna
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  5. K. Madan
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  6. B. Sireesha Rani
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  7. G. Sudhakar
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  8. G. A. Rama Raju
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Correspondence to G. A. Rama Raju.

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Sperm morphological abnormalities as predictive indicator for abnormal chromatin and DNA integrity.

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Sivanarayana, T., Krishna, C.R., Prakash, G.J. et al. CASA derived human sperm abnormalities: correlation with chromatin packing and DNA fragmentation. J Assist Reprod Genet 29, 1327–1334 (2012). https://doi.org/10.1007/s10815-012-9885-9

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  • DOI: https://doi.org/10.1007/s10815-012-9885-9

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