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The ovarian response to standard gonadotrophin stimulation depends on FSHR, SHBG and CYP19 gene synergism

  • ASSISTED REPRODUCTION TECHNOLOGIES
  • Published:
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Abstract

Purpose

Follicle stimulating hormone, sex hormone-binding globulin and cytochrome P450 aromatase play crucial roles in the regulation of mammalian reproduction. The synergistic effect of FSHR 307(T/A)/FSHR 680(N/S), SHBG(TAAAA) n and CYP19(TTTA) n genotypes on ovarian response to standard gonadotrophin stimulation of women undergoing medically assisted reproduction (IVF/ICSI) was explored.

Methods

The study population consisted of 300 women under IVF/ICSI treatment and 300 women with at least with at least one successful child birth as controls. The polymorphisms were genotyped while the follicular size, the follicle and oocyte numbers were recorded during oocyte retrieval.

Results

The genotype analysis, excluding heterozygotes for each particular polymorphism, revealed eight combined homozygotic FSHR/SHBG/CYP19 genotypes. A gradual reduction in the number of follicles and oocytes from FSHR 307Thr/680Asn allele/long SHBG allele/long CYP19 allele homozygotes to FSHR 307Ala/680Ser allele/short SHBG allele/short CYP19 allele homozygotes was observed (20.36 ± 6.74 vs. 8.05 ± 2.47, p < 0.008 and 13 ± 4.63 vs. 6.1 ± 2.32, p < 0.02, respectively).

Conclusions

FSHR/SHBG/CYP19 combined genotypes are associated with ovarian response to standard gonadotrophin stimulation of women undergoing medically assisted reproduction.

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Authors have no conflicts of interest or any financial support to declare.

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Authors and Affiliations

  1. Genetics and IVF Unit, Department of Obstetrics and Gynecology, Medical School, University of Ioannina, 45110, Ioannina, Greece

    Leandros A. Lazaros, Elissavet G. Hatzi, Prodromos I. Sakaloglou, Sophia I. Markoula, Theodoros I. Stefos, Konstantinos A. Zikopoulos & Ioannis A. Georgiou

  2. Department of Endocrinology, Medical School, Ioannina University, Ioannina, Greece

    Christina E. Pamporaki & Nectaria V. Xita

Authors
  1. Leandros A. Lazaros
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  2. Elissavet G. Hatzi
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  3. Christina E. Pamporaki
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  4. Prodromos I. Sakaloglou
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  5. Nectaria V. Xita
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  6. Sophia I. Markoula
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  7. Theodoros I. Stefos
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  8. Konstantinos A. Zikopoulos
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  9. Ioannis A. Georgiou
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Corresponding author

Correspondence to Ioannis A. Georgiou.

Additional information

Capsule The synergistic effect of FSHR, SHBG and CYP19 genetic variants on ovarian response to standard gonadotrophin stimulation of women undergoing medically assisted reproduction (IVF/ICSI).

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Lazaros, L.A., Hatzi, E.G., Pamporaki, C.E. et al. The ovarian response to standard gonadotrophin stimulation depends on FSHR, SHBG and CYP19 gene synergism. J Assist Reprod Genet 29, 1185–1191 (2012). https://doi.org/10.1007/s10815-012-9849-0

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  • DOI: https://doi.org/10.1007/s10815-012-9849-0

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