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Effect of vitrification on mitochondrial membrane potential in human metaphase II oocytes

  • Gamete Biology
  • Published:
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Abstract

Objective

The aim of this study was to evaluate the impact of vitrification on mitochondrial membrane potential (ΔΨm) in human metaphase II (MII) oocytes, and the changes of ΔΨm on thawed MII oocytes.

Methods

MII oocytes were obtained from clinical IVF cycles when the oocytes were failed to fertilization within 24 h after insemination. All oocytes were randomly divided into 4 groups: non-frozen (fresh group), cultured for 0 h (0 h group), 2 h (2 h group) and 4 h (4 h group) after vitrification/thawing. All oocytes were stained with the ΔΨm-specific probe JC-1 and detected by laser scanning confocal microscope (LSCM) for mitochondrial analysis.

Results

The ΔΨm of oocytes was significantly decreased in 0 h and 2 h groups when compared with fresh group (0.93, 1.09 vs 1.34, P < 0.05), but similar between 4 h group and fresh group (1.30 vs 1.34, P > 0.05).

Conclusion

In the vitrification/thawing process, the ΔΨm of MII oocytes could have temporally dynamic changes within 2 h after thawing but would be fully recovered after 4 h culture.

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Acknowledgements

We greatly appreciate and thank Dr De-Yi Liu from the Melbourne IVF and the University of Melbourne in Australia, for his comments and revision of the final draft of manuscript.

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Authors and Affiliations

  1. Chongqing Medical University, 1 Yi Xue Yuan ST, Yu Zhong Dis, Chongqing, 400016, China

    Cui Chen & Yaping Wang

  2. Chongqing Reproduction and Genetics Institute, Chongqing Obstetrics and Gynecology Hospital, No. 64 Jing Tang ST, Yu Zhong Dis, Chongqing, 400013, China

    Shubiao Han, Weiwei Liu & Guoning Huang

Authors
  1. Cui Chen
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  2. Shubiao Han
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  3. Weiwei Liu
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  4. Yaping Wang
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  5. Guoning Huang
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Corresponding author

Correspondence to Guoning Huang.

Additional information

Capsule The article used human unfertilized metaphase II oocytes as material to detect the recovery ability of mitochondrial membrane potential (ΔΨm) after vitrification/thawing. The results showed that the ΔΨm of MII oocytes had temporally dynamic changes within 2h after thawing but could be fully recovered after 4 h culture.

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Chen, C., Han, S., Liu, W. et al. Effect of vitrification on mitochondrial membrane potential in human metaphase II oocytes. J Assist Reprod Genet 29, 1045–1050 (2012). https://doi.org/10.1007/s10815-012-9848-1

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  • DOI: https://doi.org/10.1007/s10815-012-9848-1

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