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Optimizing cryoprotectant perfusion conditions for intact ovary: a bovine model

  • Fertility Preservation
  • Published:
Journal of Assisted Reproduction and Genetics Aims and scope Submit manuscript

Abstract

Purpose

The aim of this study was to detect the effects of different perfusion pressure and different length of perfusion period on whole ovarian cryopreservation

Methods

Bovine whole ovaries were vitrified-warmed. The ovaries were divided into the experimental groups according to different perfusion pressure and different length of perfusion period. Follicular viability was assessed using the trypan blue test; the percentage of morphologically normal primordial follicles and the 17-β estradiol level in the culture supernatants were measured.

Results

When perfusion pressure was 100 mmHg, and the length of perfusion period was 40 min, the viability of ovarian tissues in bovine whole ovarian cryopreservation were higher than other protocols.

Conclusion

Protocol IIb (the perfusion pressure was 100 mmHg, and the length of perfusion period was 40 min) was appropriate for bovine whole ovarian cryopreservation.

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Acknowledgments

We thank Dr. B Cai for helping in manuscript writing and Mrs. Mei W in ovary collection.

Conflict of interest statement

The authors declare that there are no conflicts of interest.

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Authors and Affiliations

  1. Department of Reproductive Medicine, Jinan Central Hospital Affiliated to Shandong University, 105 Jiefang Road, Jinan, China, 250000

    Jian-Min Zhang, Ying-Chun Zhang & Heng-Cai Wang

  2. Department of Obstetrics and Gynecology, Jinan Central Hospital Affiliated to Shandong University, 105 Jiefang Road, Jinan, China, 250000

    Li-Hong Ruan

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  1. Jian-Min Zhang
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  4. Heng-Cai Wang
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Correspondence to Heng-Cai Wang.

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Capsule

Bovine whole ovarian cryopreservation.

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Zhang, JM., Zhang, YC., Ruan, LH. et al. Optimizing cryoprotectant perfusion conditions for intact ovary: a bovine model. J Assist Reprod Genet 29, 1255–1260 (2012). https://doi.org/10.1007/s10815-012-9845-4

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  • DOI: https://doi.org/10.1007/s10815-012-9845-4

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