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Follicular fluid protein content (FSH, LH, PG4, E2 and AMH) and polar body aneuploidy

  • ASSISTED REPRODUCTION TECHNOLOGIES
  • Published:
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Abstract

Purpose

Our objective was to identify a marker for oocyte aneuploidy in follicular fluid (FF) in women with an increased risk of oocyte aneuploidy after controlled ovarian hyperstimulation.

Materials and methods

Three groups of oocytes were constituted for polar body screening by FISH (chromosomes 13, 16, 18, 21 and 22): Group 1, advanced maternal age (n = 156); Group 2, implantation failure (i.e. no pregnancy after the transfer of more than 10 embryos; n = 101) and Group 3, implantation failure and advanced maternal age (n = 56). FSH and other proteins were assayed in the corresponding FF samples.

Results

Of the 313 oocytes assessed, 35.78 % were abnormal. We found a significant difference between the follicular FSH levels in normal oocytes and abnormal oocytes (4.85 ± 1.75 IU/L vs. 5.41 ± 2.47 IU/L, respectively; p = 0.021). We found that the greater the number of chromosomal abnormalities per oocyte (between 0 and 3), the higher the follicular FSH level.

Conclusion

High FF FSH levels were associated with oocyte aneuploidy in women having undergone controlled ovarian hyperstimulation.

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Abbreviations

PSSC:

Premature separation of sister chromatids

ND:

Non-disjunction

PB:

Polar body

CGH:

Comparative genomic hybridization

FF:

Follicular fluid

IVF:

In vitro fertilization

ICSI:

Intra-cytoplasmic sperm injection

PGD:

Pre-implantation genetic diagnosis

FISH:

Fluorescence in situ hybridization

PGS:

Pre-implantation genetic screening

PCS:

Preconception screening

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Authors and Affiliations

  1. Service d’Histologie, Embryologie, Biologie de la Reproduction, Cytogénétique et Génétique Médicale, Centre Hospitalier Poissy St Germain en Laye, ART Center, Poissy and EA2493, Paris Ile de France, Ouest Faculty, Versailles University, 10, rue du Champ Gaillard, 78303, Poissy, France

    I. Hammoud, F. Vialard, M. Bergere, M. Albert, D. Molina Gomes, M. Bailly, R. Wainer & J. Selva

  2. Institut Mutualiste Montsouris, ART Center IMM, 42, boulevard Jourdan, 75014, Paris, France

    I. Hammoud

  3. Service de Biologie Médicale, Centre Hospitalier Poissy St Germain en Laye. 10, rue du Champ Gaillard, 78303, Poissy, France

    M. Adler & L. Malagrida

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  1. I. Hammoud
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  2. F. Vialard
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  3. M. Bergere
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  4. M. Albert
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  9. R. Wainer
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  10. J. Selva
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Correspondence to I. Hammoud.

Additional information

Capsule The higher follicular fluid FSH levels found in aneuploid oocytes (versus euploid oocytes) suggest that ovarian hyperstimulation has an effect on errors of meiosis.

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Hammoud, I., Vialard, F., Bergere, M. et al. Follicular fluid protein content (FSH, LH, PG4, E2 and AMH) and polar body aneuploidy. J Assist Reprod Genet 29, 1123–1134 (2012). https://doi.org/10.1007/s10815-012-9841-8

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