Journal of Assisted Reproduction and Genetics

, Volume 29, Issue 10, pp 1091–1096 | Cite as

Effects of EG-VEGF, VEGF and TGF-β1 on pregnancy outcome in patients undergoing IVF-ET treatment

  • Min-zhi Gao
  • Xiao-ming Zhao
  • Yi Lin
  • Zhao-gui Sun
  • Hui-qin Zhang



To investigate the correlation of endocrine gland-derived vascular endothelial growth factor (EG-VEGF), vascular endothelial growth factor (VEGF) and transforming growth factor beta 1 (TGF-β1) with the corresponding reproductive outcome in patients who received in vitro fertilization-embryo transfer (IVF-ET).


Sixty-seven women undergoing IVF-ET at a university tertiary hospital were recruited for a prospective study. Concentrations of EG-VEGF, VEGF and TGF-β1 were measured by enzyme-linked immunosorbent assay (ELISA) in follicular fluid (FF) collected during oocyte retrieval (OR) and in serum collected 2 days after OR.


In FF, concentrations of both EG-VEGF and VEGF were negatively correlated with peak E2 and the number of MII oocytes retrieved, and positively correlated with each other. In serum, concentrations of all the three growth factors were positively correlated with the rate of good quality embryo, and with one another. Patients in the pregnancy group had lower peak E2 concentrations and higher serum EG-VEGF concentrations than those in the non-pregnancy group, but such tendency was not observed in the case of VEGF and TGF-β1.


Both concentrations of EG-VEGF and VEGF in FF were negatively correlated with ovarian response and oocyte maturation. Concentrations of all the three growth factors in serum were positively correlated with embryo quality, but only serum concentrations of EG-VEGF were associated with the pregnancy outcome.





This project was supported by a research grant from School of Medicine, Shanghai Jiaotong University (2007XJ017), Shanghai, China; and the National Natural Science Foundation of China (Project No. 30672247).

Conflict of interest

The authors have no conflicts of interest.


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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Min-zhi Gao
    • 1
    • 2
  • Xiao-ming Zhao
    • 2
  • Yi Lin
    • 3
  • Zhao-gui Sun
    • 1
    • 4
  • Hui-qin Zhang
    • 1
    • 4
  1. 1.Shanghai Medical College of Fudan UniversityShanghaiChina
  2. 2.Department of Reproductive MedicineRenji Hospital, School of Medicine, Shanghai Jiaotong UniversityShanghaiChina
  3. 3.Institute of Obstetrics and GynecologyRenji Hospital, School of Medicine, Shanghai Jiaotong UniversityShanghaiChina
  4. 4.National Population and Family Planning Key Laboratory of Contraceptive Drugs & DevicesShanghai Institution of Planned Parenthood ResearchShanghaiChina

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