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Overexpression of ribosomal RNA in cumulus cells of patients with polycystic ovary syndrome

  • Gonadal Physiology and Disease
  • Published:
Journal of Assisted Reproduction and Genetics Aims and scope Submit manuscript

Abstract

Objective

To evaluate the level of ribosomal RNA (rRNA) in cumulus cells (CCs) from women with polycystic ovary syndrome (PCOS) undergoing ICSI.

Materials and methods

The study population included three healthy oocyte donors (control) and three patients with PCOS. RNA expression in CCs was assessed using quantitative real-time PCR assay to measure the pre-rRNA transcripts (5′ETS region), 18 S, 5.8 S and 28 S rRNA.

Results

The level of 18 S rRNA is 3.9 times higher (p = 0.036) and the level of 5.8 S rRNA is 2.9 times higher (p = 0.049) in CCs of PCOS patients than in CCs of healthy women. The fold change in expression of 28 S rRNA in CCs of PCOS patients also exceeds that in the control group, but did not reach a statistical significance (p = 0.342).

Conclusion

Our observations support the idea that CCs of PCOS patients contain comparatively more ribosomes that CCs of healthy oocyte donors that may indicate a higher proliferation rate or up-regulated translation of protein factors in ССs of PCOS patients.

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Acknowledgments

The study was financed by the Russian Ministry of Education and Science (grants 14.740.11.0925 and 14.740.11.0121).

Conflict of interest

None

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Authors and Affiliations

  1. Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 16/10 Miklukho-Maklaya St, 117997, Moscow, Russia

    Mikhail Polzikov & Olga Zatsepina

  2. “AltraVita” IVF clinic, 4A Nagornaya St, 117186, Moscow, Russia

    Mikhail Polzikov, Sergey Yakovenko, Julia Voznesenskaya & Maria Troshina

Authors
  1. Mikhail Polzikov
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  2. Sergey Yakovenko
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  3. Julia Voznesenskaya
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  4. Maria Troshina
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  5. Olga Zatsepina
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Corresponding author

Correspondence to Mikhail Polzikov.

Additional information

Capsule

We found that cumulus cells of women with polycystic ovary syndrome are enriched in rRNA that may argue in favor of a higher content of ribosomes and, apparently, of up-regulated total protein synthesis.

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Polzikov, M., Yakovenko, S., Voznesenskaya, J. et al. Overexpression of ribosomal RNA in cumulus cells of patients with polycystic ovary syndrome. J Assist Reprod Genet 29, 1141–1145 (2012). https://doi.org/10.1007/s10815-012-9827-6

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  • DOI: https://doi.org/10.1007/s10815-012-9827-6

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