Journal of Assisted Reproduction and Genetics

, Volume 29, Issue 10, pp 1141–1145 | Cite as

Overexpression of ribosomal RNA in cumulus cells of patients with polycystic ovary syndrome

  • Mikhail Polzikov
  • Sergey Yakovenko
  • Julia Voznesenskaya
  • Maria Troshina
  • Olga Zatsepina
Gonadal Physiology and Disease



To evaluate the level of ribosomal RNA (rRNA) in cumulus cells (CCs) from women with polycystic ovary syndrome (PCOS) undergoing ICSI.

Materials and methods

The study population included three healthy oocyte donors (control) and three patients with PCOS. RNA expression in CCs was assessed using quantitative real-time PCR assay to measure the pre-rRNA transcripts (5′ETS region), 18 S, 5.8 S and 28 S rRNA.


The level of 18 S rRNA is 3.9 times higher (p = 0.036) and the level of 5.8 S rRNA is 2.9 times higher (p = 0.049) in CCs of PCOS patients than in CCs of healthy women. The fold change in expression of 28 S rRNA in CCs of PCOS patients also exceeds that in the control group, but did not reach a statistical significance (p = 0.342).


Our observations support the idea that CCs of PCOS patients contain comparatively more ribosomes that CCs of healthy oocyte donors that may indicate a higher proliferation rate or up-regulated translation of protein factors in ССs of PCOS patients.


Cumulus cells rRNA In vitro fertilization Polycystic ovary syndrome 



The study was financed by the Russian Ministry of Education and Science (grants 14.740.11.0925 and 14.740.11.0121).

Conflict of interest



  1. 1.
    Boulon S, Westman BJ, Hutten S, Boisvert FM, Lamond AI. The nucleolus under stress. Mol Cell. 2010;40:216–27.PubMedCrossRefGoogle Scholar
  2. 2.
    Pederson T. The nucleolus. Cold Spring Harb Perspect Biol. 2011;3.Google Scholar
  3. 3.
    Wolgemuth DJ, Jagiello GM, Henderson AS. Quantitation of ribosomal RNA genes in fetal human oocyte nuclei using rRNA: DNA hybridization in situ. Evidence for increased multiplicity. Exp Cell Res. 1979;118:181–90.PubMedCrossRefGoogle Scholar
  4. 4.
    Boisvert FM, van Koningsbruggen S, Navascues J, Lamond AI. The multifunctional nucleolus. Nat Rev Mol Cell Biol. 2007;8:574–85.PubMedCrossRefGoogle Scholar
  5. 5.
    Assou S, Haouzi D, De Vos J, Hamamah S. Human cumulus cells as biomarkers for embryo and pregnancy outcomes. Mol Hum Reprod. 2010;16:531–8.PubMedCrossRefGoogle Scholar
  6. 6.
    Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 2001;25:402–8.PubMedCrossRefGoogle Scholar
  7. 7.
    Rotterdam ESHRE/ASRM-Sponsored PCOS consensus workshop group. Revised 2003 consensus on diagnostic criteria and long-term health risks related to polycystic ovary syndrome (PCOS). Hum Reprod. 2004;19:41–7.CrossRefGoogle Scholar
  8. 8.
    McVeigh E, Homburg R, Guillebaud J. Polycystic ovary syndrome. In: Oxford Handbook of Reproductive Medicine and Family Planning. Oxford University Press; 2008:57–71.Google Scholar
  9. 9.
    Derenzini M, Trere D, Pession A, Montanaro L, Sirri V, Ochs RL. Nucleolar function and size in cancer cells. Am J Pathol. 1998;152:1291–7.PubMedGoogle Scholar
  10. 10.
    McStay B, Grummt I. The epigenetics of rRNA genes: from molecular to chromosome biology. Annu Rev Cell Dev Biol. 2008;24:131–57.PubMedCrossRefGoogle Scholar
  11. 11.
    Das M, Djahanbakhch O, Hacihanefioglu B, Saridogan E, Ikram M, Ghali L, Raveendran M, Storey A. Granulosa cell survival and proliferation are altered in polycystic ovary syndrome. J Clin Endocrinol Metab. 2008;93:881–7.PubMedCrossRefGoogle Scholar
  12. 12.
    Huang Z, Wells D. The human oocyte and cumulus cells relationship: new insights from the cumulus cell transcriptome. Mol Hum Reprod. 2010;16:715–25.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Mikhail Polzikov
    • 1
    • 2
  • Sergey Yakovenko
    • 2
  • Julia Voznesenskaya
    • 2
  • Maria Troshina
    • 2
  • Olga Zatsepina
    • 1
  1. 1.Shemyakin-Ovchinnikov Institute of Bioorganic ChemistryRussian Academy of SciencesMoscowRussia
  2. 2.“AltraVita” IVF clinicMoscowRussia

Personalised recommendations