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Cumulus cell-oocyte complexes retrieved from antral follicles in IVM cycles: relationship between COCs morphology, gonadotropin priming and clinical outcome

  • Mariabeatrice Dal Canto
  • Fausta Brambillasca
  • Mario Mignini Renzini
  • Giovanni Coticchio
  • Maria Merola
  • Monia Lain
  • Elena De Ponti
  • Rubens Fadini
ASSISTED REPRODUCTION TECHNOLOGIES

Abstract

Purpose

To assess retrospectively the developmental potential of different types of cumulus cell-oocyte complexes (COCs) derived from IVM cycles.

Methods

IVM cycles were performed in natural cycles or after HCG, FSH, or FSH/HCG priming. COCs recovered were morphologically characterized in different types: compact (CC) or expanded (EC) cumulus mass but including an immature oocyte, and expanded cumulus mass enclosing a mature oocyte (EC-MII). Embryo developmental competence was investigated analysing exclusively cycles in which all transferred embryos derived from the same COC category.

Results

Fertilization rates did not differ significantly. Significant differences in pregnancy rates (14.5 %, 10.0 % and 27.6 % in the CC, EC, and EC-MII categories, respectively) were observed. Likewise, significant differences in implantation rates (8.9 %, 6.3 % and 19.1 % in the CC, EC, and EC-MII categories, respectively) were found. Overall, priming with FSH/HCG had a beneficial effect on pregnancy and implantation rates, while no priming or HCG alone generated oocytes with poor competence.

Conclusions

In IVM cycles, morphological evaluation at the time of collection can predict the developmental ability of different COCs. FSH/HGC priming has a positive effect on oocyte competence.

Keywords

Oocytes Cumulus cell-oocyte complexes In vitro maturation Pregnancy Implantation 

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Mariabeatrice Dal Canto
    • 1
  • Fausta Brambillasca
    • 1
  • Mario Mignini Renzini
    • 1
  • Giovanni Coticchio
    • 1
  • Maria Merola
    • 1
  • Monia Lain
    • 1
  • Elena De Ponti
    • 2
  • Rubens Fadini
    • 1
  1. 1.Biogenesi Reproductive Medicine CentreIstituti Clinici ZucchiMonzaItaly
  2. 2.Department of Medical PhysicsSan Gerardo HospitalMonzaItaly

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