Release of superoxide dismutase-1 by day 3 embryos of varying quality and implantation potential
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To determine if the antioxidant superoxide dismutase-1 (SOD1 or Cu,Zn-SOD) is released by cultured human cleavage-stage embryos and to assess any link between SOD1 and implantation potential.
Women (n = 91; ≤40 years old) undergoing IVF treatment with transfer of one or two 8-cell embryos that resulted in 0 or 100% implantation were included. Following individual embryo culture, spent medium samples (n = 122) were collected and levels of SOD1 protein were measured by an enzyme-linked immunosorbent assay. SOD1 detection and concentration in embryo spent medium were analyzed in relation to embryo fragmentation and symmetry scores, and implantation (viable fetus at >12 weeks).
Cleavage-stage embryos release SOD1 protein into the spent culture medium. Neither detection nor concentration of SOD1 was related to implantation. There was a positive relationship between increased embryo fragmentation scores and SOD1 release, with no apparent association with symmetry. In non-pregnant cycles, the release of SOD1 decreased with increasing maternal age.
While SOD1 does not predict implantation potential of select good-quality embryos, our data support the need to evaluate the biological significance of released SOD1 by embryos of varying quality and from patients of varying age.
KeywordsEmbryo culture medium Secreted protein Superoxide dismutase-1 Cu,Zn-superoxide dismutase Pregnancy outcome
- 1.Cummins JM, Breen TM, Harrison KL, Shaw JM, Wilson LM, Hennessey JF. A formula for scoring human embryo growth rates in in vitro fertilization: its value in predicting pregnancy and in comparison with visual estimates of embryo quality. J In Vitro Fert Embryo Transf. 1986;3:284–95.CrossRefPubMedGoogle Scholar
- 4.Holte J, Berglund L, Milton K, Garello C, Gennarelli G, Revelli A, Bergh T. Construction of an evidence-based integrated morphology cleavage embryo score for implantation potential of embryos scored and transferred on day 2 after oocyte retrieval. Hum Reprod. 2007;22:548–57.CrossRefPubMedGoogle Scholar
- 16.Seli E, Sakkas D, Scott R, Kwok SC, Rosendahl SM, Burns DH. Noninvasive metabolomic profiling of embryo culture media using Raman and near-infrared spectroscopy correlates with reproductive potential of embryos in women undergoing in vitro fertilization. Fertil Steril. 2007;88:1350–7.CrossRefPubMedGoogle Scholar
- 21.Wiener-Megnazi Z, Shiloh H, Avraham L, Lahav-Baratz S, Koifman M, Reznick AZ, Auslender R, Dirnfeld M. Oxidative parameters of embryo culture media may predict treatment outcome in in vitro fertilization: a novel applicable tool for improving embryo selection. Fertil Steril. 2011;95:979–84.CrossRefPubMedGoogle Scholar
- 27.Santillo M, Secondo A, Seru R, Damiano S, Garbi C, Taverna E, Rosa P, Giovedi S, Benfenati F, Mondola P. Evidence of calcium- and SNARE-dependent release of CuZn superoxide dismutase from rat pituitary GH3 cells and synaptosomes in response to depolarization. J Neurochem. 2007;102:679–85.CrossRefPubMedGoogle Scholar
- 41.Tarin JJ, Gomez-Piquer V, Pertusa JF, Hermenegildo C, Cano A. Association of female aging with decreased parthenogenetic activation, raised MPF, and MAPKs activities and reduced levels of glutathione S-transferases activity and thiols in mouse oocytes. Mol Reprod Dev. 2004;69:402–10.CrossRefPubMedGoogle Scholar
- 43.Tatone C, Carbone MC, Falone S, Aimola P, Giardinelli A, Caserta D, Marci R, Pandolfi A, Ragnelli AM, Amicarelli F. Age-dependent changes in the expression of superoxide dismutases and catalase are associated with ultrastructural modifications in human granulosa cells. Mol Hum Reprod. 2006;12:655–60.CrossRefPubMedGoogle Scholar