Journal of Assisted Reproduction and Genetics

, Volume 29, Issue 3, pp 277–279 | Cite as

Live birth from oocytes cryopreserved with slow-freezing protocol and thawed after 6 years of storage

  • Giovanni Battista La Sala
  • Francesco Capodanno
  • Barbara Valli
  • Ilaria Rondini
  • Maria Teresa Villani
  • Alessia Nicoli
Fertility Preservation


After the birth of more than 900 babies from human cryopreserved oocytes with no apparent increase in congenital anomalies compared to conventional IVF babies [1], oocyte cryopreservation has become a quite widespread technique in Assisted Reproductive Technologies (ART), even in the female fertility preservation programs. Today two procedures are available for oocyte cryopreservation: slow-freezing (SF), the first protocol introduced, and vitrification (VT) [2]. The main goal of VT is to achieve high cryoprotectant concentration in order to increase the viscosity of the cryoprotectant solution and to suppress ice nucleation [3]. Biological and clinical outcomes of SF protocols seem to be less than those obtained with fresh and vitrified oocytes [4, 5, 6]. Oocyte cryopreservation is usually used in ART laboratories for supernumerary oocyte storage after Controlled Ovarian Stimulation (COS) [4], prevention of Ovarian HyperStimulation Syndrome (OHSS) risk, oocyte...


Assist Reproductive Technology Fertility Preservation Ovarian HyperStimulation Syndrome Embryo Cryopreservation Oocyte Cryopreservation 
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Conflict of interest

The Authors declare no potential conflict of interests.


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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Giovanni Battista La Sala
    • 1
  • Francesco Capodanno
    • 2
  • Barbara Valli
    • 2
  • Ilaria Rondini
    • 2
  • Maria Teresa Villani
    • 2
  • Alessia Nicoli
    • 2
  1. 1.Department of Obstetrics and GynecologyUniversity of Modena and Reggio EmiliaReggio EmiliaItaly
  2. 2.Department of Obstetrics and GynecologyReggio EmiliaItaly

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