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Male infertility in Northeast China: a cytogenetic study of 135 patients with non-obstructive azoospermia and severe oligozoospermia

  • Genetics
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Abstract

Purposes

To detect the frequency and types of chromosomal anomalies with non-obstructive azoospermia and severe oligozoospermia in Northeast China, and to compare the frequencies with other regions of China and the world. To investigate the general characteristics of this population.

Methods

Eighty-one men with non-obstructive azoospermia and 54 men with severe oligozoospermia were recruited. Karyotype analyses were performed on peripheral blood lymphocytes using standard G-banding. Measurements of follicle-stimulating hormone, testosterone, prolactin, and inhibin B were obtained.

Results

The frequency of chromosomal anomalies for patients with non-obstructive azoospermia (17.28%) was comparable with studies from Europe, Africa, Asia, and other regions of China. However, the frequency for patients with severe oligozoospermia (9.26%) was slightly higher than reported from other Asian countries. The infertile men were more likely than the fertile to smoke and consume alcohol, and to have significantly lower levels of inhibin B.

Conclusions

For infertile men in Northeast China, chromosome analysis is a necessary part of routine genetic testing, and the contributing effects of high smoking and alcohol consumption rates of this population should be discussed during genetic counseling.

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References

  1. Balkan M, Tekes S, Gedik A. Cytogenetic and Y chromosome microdeletion screening studies in infertile males with oligozoospermia and azoospermia in Southeast Turkey. J Assist Reprod Genet. 2008;25:559–65.

    Article  PubMed  CAS  Google Scholar 

  2. Kleiman SE, Yogev L, Gamzu R, Hauser R, Botchan A, Lessing JB, Paz G, Yavetz H. Genetic evaluation of infertile men. Hum Reprod. 1999;14(1):33–8.

    Article  PubMed  CAS  Google Scholar 

  3. Ceylan GG, Ceylan C, Elyas H. Genetic anomalies in patients with severe oligozoospermia and azoospermia in eastern Turkey: a prospective study. Genet Mol Res. 2009;8(3):915–22.

    Article  PubMed  CAS  Google Scholar 

  4. Kosar PA, Ozcelik N, Kosar A. Cytogenetic abnormalities detected in patients with non-obstructive azoospermia and severe oligozoospermia. J Assist Reprod Genet. 2010;27:17–21.

    Article  PubMed  Google Scholar 

  5. Dohle GR, Halley DJ, Van Hemel JO, van den Ouwel AM, Pieters MH, Weber RF, Govaerts LC. Genetic risk factors in infertile men with severe oligozoospermia and azoospermia. Hum Reprod. 2002;17(1):13–6.

    Article  PubMed  CAS  Google Scholar 

  6. Martin RH. Cytogenetic determinants of male fertility. Hum Reprod Updat. 2008;14(4):379–90.

    Article  CAS  Google Scholar 

  7. Foresta C, Garolla A, Bartoloni L, Bettella A, Ferlin A. Genetic abnormalities among severely oligospermic men who are candidates for intracytoplasmic sperm injection. J Clin Endocrinol Metab. 2005;90(1):152–6.

    Article  PubMed  CAS  Google Scholar 

  8. Elghezal H, Hidar S, Braham R, Denguezli W, Ajina M, Saâd A. Chromosome abnormalities in 1,000 infertile males with nonobstructive sperm disorders. Fertil Steril. 2006;86(6):1792–5.

    Article  PubMed  Google Scholar 

  9. Mohammed F, Al-Yatama F, Al-Bader M, Tayel SM, Gouda S, Naguib KK. Primary male infertility in Kuwait: a cytogenetic and molecular study of 289 infertile Kuwaiti patients. Andrologia. 2007;39(3):87–92.

    Article  PubMed  CAS  Google Scholar 

  10. Vutyavanich T, Piromlertamorn W, Sirirungsi W, Sirisukkasem S. Frequency of Y chromosome microdeletions and chromosomal abnormalities in infertile Thai men with oligozoospermia and azoospermia. Asian J Androl. 2007;9(1):68–75.

    Article  PubMed  CAS  Google Scholar 

  11. Nagvenkar P, Desai K, Hinduja I, Zaveri K. Chromosomal studies in infertile men with oligozoospermia & non-obstructive azoospermia. Indian J Med Res. 2005;122(1):34–42.

    PubMed  Google Scholar 

  12. Ng PP, Tang MH, Lau ET, Ng LK, Ng EH, Tam PC, Yeung WS, Ho PC. Chromosomal anomalies and Y-microdeletions among Chinese subfertile men in Hong Kong. Hong Kong Med J. 2009;15(1):31–8.

    PubMed  Google Scholar 

  13. Chiang HS, Wei HJ, Chen YT. Genetic screening for patients with azoospermia and severe oligo-asthenospermia. Int J Androl. 2000;23(2):20–5.

    Article  PubMed  Google Scholar 

  14. Zhou-Cun A, Yang Y, Zhang SZ, Zhang W, Lin L. Chromosomal abnormality and Y chromosome microdeletion in Chinese patients with azoospermia or severe oligozoospermia. Yi Chuan Xue Bao. 2006;33(2):111–6.

    PubMed  CAS  Google Scholar 

  15. World Health Organization. WHO laboratory manual for the examination of human semen and sperm-cervical mucus interaction. 4th ed. Cambridge: Cambridge University Press; 1999.

    Google Scholar 

  16. Mitelman F, ISCN, editors. An international system for human cytogenetic nomenclature. Basel: Karger; 1995.

    Google Scholar 

  17. Wang RX, Fu C, Yang YP, Han RR, Dong Y, Dai RL, Liu RZ. Male infertility in China: laboratory finding for AZF microdeletions and chromosomal abnormalities in infertile men from Northeastern China. J Assist Reprod Genet. 2010;27(7):391–6.

    Article  PubMed  CAS  Google Scholar 

  18. Myers GM, Lambert-Messerlian GM, Sigman M. Inhibin B reference data for fertile and infertile men in Northeast America. Fertil Steril. 2009;92(6):1920–3.

    Article  PubMed  CAS  Google Scholar 

  19. Pierik FH, Vreeburg JT, Stijnen T, De Jong FH, Weber RF. Serum inhibin B as a marker of spermatogenesis. J Clin Endocrinol Metab. 1998;83(9):3110–4.

    Article  PubMed  CAS  Google Scholar 

  20. Kumanov P, Nandipati K, Tomova A, Agarwal A. Inhibin B is a better marker of spermatogenesis than other hormones in the evaluation of male factor infertility. Fertil Steril. 2006;86(2):328–32.

    Article  Google Scholar 

  21. Vine MF, Margolin BH, Morrison HI, Hulka BS. Cigarette smoking and sperm density: a meta-analysis. Fertil Steril. 1994;61:35–43.

    PubMed  CAS  Google Scholar 

  22. Giwercman A, Giwercman YL. Environmental factors and testicular function. Best Pract Res Clin Endocrin Metab. 2011;25:391–402.

    Article  CAS  Google Scholar 

  23. Martini AC, Molina RI, Estofán D, Senestrari D, Fiol de Cuneo M, Ruiz RD. Effects of alcohol and cigarette consumption on human seminal quality. Fertil Steril. 2004;82(2):374–7.

    Article  PubMed  Google Scholar 

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Acknowledgements

We are grateful to all the patients and donors of blood samples. We thank the excellent work of all the staff of the Andrology Laboratory. We appreciate Professor Frederick William Orr for his general English-language assistance and timely support. The work of this study was kindly supported by funds from the Office of Science and Technology project in Jilin Province, China (NO. 20080444–3).

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Authors and Affiliations

  1. First Hospital of Jilin University, Changchun, 130021, China

    Zhi-Bo Zhang, Yu-Ting Jiang, Xin Yun, Xiao Yang, Rui-Xue Wang, Ru-Lin Dai & Rui-Zhi Liu

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  1. Zhi-Bo Zhang
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  2. Yu-Ting Jiang
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  3. Xin Yun
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  4. Xiao Yang
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  5. Rui-Xue Wang
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  6. Ru-Lin Dai
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  7. Rui-Zhi Liu
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Corresponding author

Correspondence to Rui-Zhi Liu.

Additional information

Capsule For infertile men in Northeast China, it is essential to do a chromosome analysis and pay attention to the contributing effects of high smoking and alcohol consumption rates during routine genetic work.

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Zhang, ZB., Jiang, YT., Yun, X. et al. Male infertility in Northeast China: a cytogenetic study of 135 patients with non-obstructive azoospermia and severe oligozoospermia. J Assist Reprod Genet 29, 83–87 (2012). https://doi.org/10.1007/s10815-011-9670-1

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  • DOI: https://doi.org/10.1007/s10815-011-9670-1

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