Journal of Assisted Reproduction and Genetics

, Volume 28, Issue 11, pp 1099–1104 | Cite as

Relationship between meiotic spindle characteristics in human oocytes and the timing of the first zygotic cleavage after intracytoplasmic sperm injection

  • Hiroyuki Tomari
  • Kou Honjou
  • Yumi Nagata
  • Toshitaka Horiuchi
Embryo Biology



To investigate the relationship between meiotic spindle characteristics in human oocytes and the timing of the first zygotic cleavage after intracytoplasmic sperm injection (ICSI).


Zygotes that had cleaved to two-cell stage by 27 h post-ICSI were classified as early cleaving and the remainder as late cleaving. Meiotic spindle parameters previously imaged using the PolScope were compared between the two groups.


Of 384 embryos, 163 were classed as early cleaving and 221 as late cleaving. The rate of blastocyst formation or pregnancy by Day 2 embryo transfer was significantly higher following early cleavage than after late cleavage (52.4% vs. 24.4% or 32.6% vs. 11.4%). Spindle areas (108.0 vs. 89.8 μm2), lengths (14.7 vs. 13.4 μm) and PolScope retardance were also significantly greater in the early cleaving group.


Meiotic spindle parameters determine the timing of the first zygotic cleavage and are strong indicators of human embryo developmental potential.


Early cleavage Meiotic spindle Embryo quality PolScope Human oocytes 


  1. 1.
    Pandian Z, Templeton A, Serour G, Bhattacharya S. Number of embryos for transfer after IVF and ICSI: a Cochrane review. Hum Reprod. 2005;20:2681–7.PubMedCrossRefGoogle Scholar
  2. 2.
    Vilska S, Tiitinen A, Hydén-Granskog C, Hovatta O. Elective transfer of one embryo results in an acceptable pregnancy rate and eliminates the risk of multiple birth. Hum Reprod. 1999;14:2392–5.PubMedCrossRefGoogle Scholar
  3. 3.
    Gerris J, De Neubourg D, Mangelschots K, Van Royen E, Vercruyssen M, Barudy-Vasquez J, et al. Elective single day 3 embryo transfer halves the twinning rate without decrease in the ongoing pregnancy rate of an IVF/ICSI programme. Hum Reprod. 2002;17:2626–31.PubMedCrossRefGoogle Scholar
  4. 4.
    Rienzi L, Ubaldi F, Iacobelli M, Ferrero S, Minasi MG, Martinez F, et al. Day 3 embryo transfer with combined evaluation at the pronuclear and cleavage stages compares favorably with day 5 blastocyst transfer. Hum Reprod. 2002;17:1852–5.PubMedCrossRefGoogle Scholar
  5. 5.
    Milki AA, Jun SH, Hinckley MD, Behr B, Giudice LC, Westphal LM. Incidence of monozygotic twinning with blastocyst transfer compared to cleavage-stage transfer. Fertil Steril. 2003;79:503–6.PubMedCrossRefGoogle Scholar
  6. 6.
    Knopman J, Krey LC, Lee J, Fino ME, Novetsky AP, Noyes N. Monozygotic twinning: an eight-year experience at a large IVF center. Fertil Steril. 2010;94:502–10.PubMedCrossRefGoogle Scholar
  7. 7.
    Scott LA, Smith S. The successful use of pronuclear embryo transfer the day following oocyte retrieval. Hum Reprod. 1998;13:1003–13.PubMedCrossRefGoogle Scholar
  8. 8.
    Tesarik J, Greco E. The probability of abnormal preimplantation development can be predicted by a single static observation on pronuclear stage morphology. Hum Reprod. 1999;14:1318–23.PubMedCrossRefGoogle Scholar
  9. 9.
    Giorgetti C, Terriou P, Auquier P, Hans E, Spach J-L, Salzmann J, et al. Embryo score to predict implantation after in-vitro fertilization: based on 957 single embryo transfer. Hum Reprod. 1995;10:2427–31.PubMedGoogle Scholar
  10. 10.
    Hardarson T, Hanson C, Sjögren A, Lundin K. Human embryos with unevenly sized blastomeres have lower pregnancy and implantation rates: indications for aneuploidy and multinucleation. Hum Reprod. 2001;16:313–8.PubMedCrossRefGoogle Scholar
  11. 11.
    Shoukir Y, Campana A, Farley T, Sakkas D. Early cleavage of in-vitro fertilized human embryos to the 2-cell stage: a novel indicator of embryo quality and viability. Hum Reprod. 1997;12:1531–6.PubMedCrossRefGoogle Scholar
  12. 12.
    Sakkas D, Shoukir Y, Chardonnens D, Bianchi PG, Campana A. Early cleavage of human embryos to the two-cell stage after intracytoplasmic sperm injection as an indicator of embryo viability. Hum Reprod. 1998;13:182–7.PubMedCrossRefGoogle Scholar
  13. 13.
    Rienzi L, Ubaldi F, Martinez F, Iacobelli M, Minasi MG, Ferrero S, et al. Relationship between meiotic spindle location with regard to the polar body position and oocyte developmental potential after ICSI. Hum Reprod. 2003;18:1289–93.PubMedCrossRefGoogle Scholar
  14. 14.
    Cohen Y, Malcov M, Schwartz T, Mey-Raz N, Carmon A, Cohen T, et al. Spindle imaging: a new marker for optional timing of ICSI? Hum Reprod. 2004;19:649–54.PubMedCrossRefGoogle Scholar
  15. 15.
    Fenwick J, Platteau P, Murdoch AP, Herbert M. Time from insemination to first cleavage predicts developmental competence of human preimplantation embryos in vitro. Hum Reprod. 2002;17:407–12.PubMedCrossRefGoogle Scholar
  16. 16.
    Van Montfoort APA, Dumoulin JCM, Kester ADM, Evers JLH. Early cleavage is a valuable addition to existing embryo selection parameters: a study using single embryo transfers. Hum Reprod. 2004;19:2103–8.PubMedCrossRefGoogle Scholar
  17. 17.
    Lundin K, Bergh C, Hardarson T. Early embryo cleavage is a strong indicator of embryo quality in human IVF. Hum Reprod. 2001;16:2652–7.PubMedCrossRefGoogle Scholar
  18. 18.
    Salumets A, Hydén-Granskog C, Mäkinen S, Suikkari A-M, Tiitinen A, Tuuri T. Early cleavage predicts the viability of human embryos in elective single embryo transfer procedures. Hum Reprod. 2003;18:821–5.PubMedCrossRefGoogle Scholar
  19. 19.
    Nagy ZP, Liu J, Joris H, Verheyen G, Tournaye H, Camus M, et al. The result of intracytoplasmic sperm injection is not related to any of the three basic sperm parameters. Hum Reprod. 1995;10:1123–9.PubMedGoogle Scholar
  20. 20.
    Van Blerkom J, Davis PW, Lee J. ATP content of human oocytes and developmental potential and outcome after in-vitro fertilization and embryo transfer. Hum Reprod. 1995;10:415–24.PubMedGoogle Scholar
  21. 21.
    Vogt E, Kirsch-Volders M, Parry J, Eichenlaub-Ritter U. Spindle formation, chromosome segration and the spindle checkpoint in mammalian oocytes and susceptibility to meiotic error. Mutat Res. 2008;651:14–29.PubMedGoogle Scholar
  22. 22.
    Sanfins A, Lee GY, Plancha CE, Overstrom EW, Albertini DF. Distinctions in meiotic spindle structure and assembly during in vitro and in vivo maturation of mouse oocytes. Biol Reprod. 2003;69:2059–67.PubMedCrossRefGoogle Scholar
  23. 23.
    Keefe D, Liu L, Wang W, Silva C. Imaging meiotic spindles by polarization light microscopy: principles and application to IVF. Reprod Biomed Online. 2003;7:24–9.PubMedCrossRefGoogle Scholar
  24. 24.
    Moon J-H, Hyun C-S, Lee S-W, Son W-Y, Yoon S-H, Lim J-H. Visualization of the metaphase II meiotic spindle in living human oocytes using the Polscope enables the prediction of embryonic developmental competence after ICSI. Hum Reprod. 2003;18:817–20.PubMedCrossRefGoogle Scholar
  25. 25.
    Konc J, Kanyó K, Cseh S. Visualization and examination of the meiotic spindle in human oocytes with Polscope. J Assist Reprod Genet. 2004;21:349–53.PubMedCrossRefGoogle Scholar
  26. 26.
    Madaschi C, de Souza Bonetti TC, de Almeida Ferreira Braga DP, Pasqualotto FF, Iaconelli Jr A, Borges Jr E. Spindle imaging: a marker for embryo development and implantation. Fertil Steril. 2008;90:194–8.PubMedCrossRefGoogle Scholar
  27. 27.
    Cooke S, Tyler JPP, Driscoll GL. Meiotic spindle location and identification and its effect on embryonic cleavage plane and early development. Hum Reprod. 2003;18:2397–405.PubMedCrossRefGoogle Scholar
  28. 28.
    Shen Y, Stalf T, Mehnert C, De Santis L, Cino I, Tinneberg HR, et al. Light retardance by human oocyte spindle is positively related to pronuclear score after ICSI. Reprod Biomed Online. 2006;12:737–51.PubMedCrossRefGoogle Scholar
  29. 29.
    Fang C, Tang M, Li T, Peng WL, Zhou CQ, Zhuang GL, et al. Visualization of meiotic spindle and subsequent embryonic development in in vitro and in vivo matured human oocytes. J Assist Reprod Genet. 2007;24:547–51.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Hiroyuki Tomari
    • 1
    • 2
  • Kou Honjou
    • 1
  • Yumi Nagata
    • 1
  • Toshitaka Horiuchi
    • 2
  1. 1.IVF Nagata ClinicFukuokaJapan
  2. 2.Graduate School of Comprehensive Scientific ResearchPrefectural University of HiroshimaHiroshimaJapan

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