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Protein tyrosine phosphatase non-receptor type 14 is a novel sperm-motility biomarker

  • Gamete Biology
  • Published:
Journal of Assisted Reproduction and Genetics Aims and scope Submit manuscript

Abstract

Purpose

To understand the molecular basis of sperm-motility and to identify related novel motility biomarkers.

Methods

Two-dimensional electrophoresis (2DE) followed by Reverse-phase-nano-high-performance liquid chromatography-electrospray ionization tandem mass spectrometry (RP-nano-HPLC-ESI-MS/MS) were applied to establish the human sperm proteome. Then the sperm proteome of moderate-motile human sperm fraction and that of good-motile human sperm fraction from pooled spermatozoa of forty normozoospermic donors (Group 1 subjects) were compared to identify the dysregulated proteins. Among these down-regulated proteins, Protein tyrosine phosphatase non-receptor type 14 (PTPN14) was chosen to reconfirm by Western blotting and semi-quantitative reverse transcription polymerase chain reaction. For clinical application, Western blotting and real-time reverse transcription polymerase chain reaction was performed to compare the expression level of PTPN14 in (Group 2 subjects) nine normozoospermic controls and thirty-three asthenozoospermic patients (including 21 mild asthenozoospermic cases and 12 severe cases). Finally, bioinformatic tools prediction and immunofluorescence assay were performed to elucidate the potential localization of PTPN14.

Results

The expression levels of three proteins were observed to be lower in the moderate-motile sperm fraction than in good-motile sperm of group 1 subjects. Among three proteins with persistent down-regulation in the moderate-motile sperm, we reconfirmed that the expression level of PTPN14 was significantly lower in both mRNA and protein levels from the moderate-motile sperm fraction. Further, down-regulation of PTPN14 was found at the translational and transcriptional level in the asthenozoospermic men. Finally, Bioinformatic tools prediction and immunofluorescence assay showed that PTPN14 maybe predominantly localized at the mitochondria in the midpiece of human ejaculated sperm.

Conclusions

Proteomics tools were applied to identify three possible sperm motility-related proteins. Among these proteins, PTPN14 was highly likely a novel sperm-motility biomarker and a potential mitochondrial protein.

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Acknowledgement

This study was supported by grants from the National Science Council of the Republic of China (NSC- 91-2314-B-006-149, NSC 91-3112-B-006-008, NSC 92-3112-B-006-002, NSC 93-3112-B-006-004, and NSC 93-2314-B-006-078).

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Authors and Affiliations

  1. Division of Obstetrics and Gynecology, National Cheng Kung University College of Medicine and Hospital, Dou-Liou Branch, Yunlin, Taiwan

    Hsin-Chih Albert Chao

  2. Department of Obstetrics & Gynecology, National Cheng Kung University College of Medicine and Hospital, Tainan, Taiwan

    Chia-Ling Chung, Hsien-An Pan & Pao-Lin Kuo

  3. Department of Environmental and Occupational Medicine, National Cheng Kung University College of Medicine, Tainan, Taiwan

    Pao-Chi Liao

  4. Department of Obstetrics & Gynecology, China Medical University, Taichung, Taiwan

    Chao-Chin Hsu

  5. College of Medicine, E-Shou University, Kaohsiung, Taiwan No.91 Hsueh-Shih Road, Taichung, Taiwan, 40402, Republic of China

    Chao-Chin Hsu

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  1. Hsin-Chih Albert Chao
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  2. Chia-Ling Chung
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Correspondence to Chao-Chin Hsu.

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Protein tyrosine phosphatase non-receptor type 14 is a novel sperm-motility biomarker proven by proteomic tools.

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Supplemental Table 1

Parameters for good-motility and moderate-motility sperm fractions which were separated by density gradients and used for proteomics study. (DOC 29 kb)

Supplemental Table 2

Sperm parameters of normozoospermic controls and men with asthenozoospermia. (DOC 30 kb)

Supplemental Table 3

Proteins which are persistently under-expressed in moderate-motility sperm. (DOC 29 kb)

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Chao, HC.A., Chung, CL., Pan, HA. et al. Protein tyrosine phosphatase non-receptor type 14 is a novel sperm-motility biomarker. J Assist Reprod Genet 28, 851–861 (2011). https://doi.org/10.1007/s10815-011-9602-0

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  • DOI: https://doi.org/10.1007/s10815-011-9602-0

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