Abstract
Purpose
To identify differentially expressed microRNAs (miRNAs) and expression patterns of specific miRNAs during meiosis in human oocytes.
Materials and methods
To identify differentially expressed miRNAs, GV oocytes and MII oocytes matured at conventional FSH levels (5.5 ng/ml) were analyzed by miRNA microarray. Real-time RT-PCR was used to confirm the changed miRNAs. To validate the dynamic changes of miRNAs from GV to MII stages, oocytes were divided into four groups (#1–4), corresponding to GV oocytes, MI oocytes, MII oocytes matured in conventional FSH level and MII oocytes matured in high FSH level (2,000 ng/ml) respectively.
Results
Compared with GV oocytes, MII oocytes exhibited up-regulation of 4 miRNAs (hsa-miR-193a-5p, hsa-miR-297, hsa-miR-625 and hsa-miR-602), and down-regulation of 11 miRNAs (hsa-miR-888*, hsa-miR-212, hsa-miR-662, hsa-miR-299-5p, hsa-miR-339-5p, hsa-miR-20a, hsa-miR-486-5p, hsa-miR-141*, hsa-miR-768-5p, hsa-miR-376a and hsa-miR-15a). RT-PCR analysis of hsa-miR-15a and hsa-miR-20a expression revealed concordant dynamic changes in oocytes from group 1 to group 4.
Conclusion(s)
Specific miRNAs in human oocytes had dynamic changes during meiosis. High-concentration FSH in IVM medium led to reverse effect on the expression of hsa-miR-15a and hsa-miR-20a.
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Yan-Wen Xu and Bin Wang contributed equally to this study.
This study was supported by National Basic Research Program of China (grant no. 2007CB948101), National Natural Science Foundation of China (grant no. 30700910 and 31071272).
Capsule
Differentially expressed miRNAs in the human oocytes between GV and MII stages were identified by miRNA microarrays. Dynamic changes of miR-15a, hsa-miR-20a, and miR-602 during meiosis were validated by qRT-PCR.
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Xu, YW., Wang, B., Ding, CH. et al. Differentially expressed micoRNAs in human oocytes. J Assist Reprod Genet 28, 559–566 (2011). https://doi.org/10.1007/s10815-011-9590-0
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DOI: https://doi.org/10.1007/s10815-011-9590-0