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Reliable preimplantation genetic diagnosis in thawed human embryos vitrified at cleavage stages without biopsy

  • Genetics
  • Published:
Journal of Assisted Reproduction and Genetics Aims and scope Submit manuscript

Abstract

Purpose

To evaluate preimplantation genetic diagnosis (PGD) efficiency in thawed human embryos vitrified without biopsy.

Methods

In this retrospective clinical study, 21 PGD cycles were carried out using fresh and vitrified-thawed embryos collected from 21 patients.

Results

One hundred and ninety-nine embryos from patients with aneuploidy, single gene defects, or chromosomal translocations were vitrified at the cleavage stages; 93.5% of the embryos survived the vitrification procedure. Conclusive FISH results were obtained in 98.4% of the fresh and 99% of the frozen-thawed embryos screened for aneuploidy or chromosomal translocations. Conclusive PCR test results were obtained in 100% of the fresh and 93.9% of the frozen-thawed embryos screened for single gene defects. The overall clinical pregnancy rate per cycle was 57.1%. To date, 13 healthy children have been born.

Conclusion

Reliable genetic diagnosis can be performed in thawed human embryos vitrified without biopsy. However, further research is required to support this conclusion.

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Author information

Authors and Affiliations

  1. MUHC Reproductive Center, Royal Victoria Hospital, and Department of Obstetrics and Gynecology, McGill University, Montreal, Quebec, Canada

    Li Zhang, Ahmet Yilmaz, Ri-Cheng Chian, Weon-Young Son, Xiao Yun Zhang, Dewen Kong, Michael Dahan, Hannanel Holzer, Seang Lin Tan & Asangla Ao

  2. Department of Human Genetics, McGill University, Montreal, Quebec, Canada

    Asangla Ao

  3. Royal Victoria Hospital, 687 Pine Avenue West F3-16, Montreal, Quebec, Canada, H3A 1A1

    Asangla Ao

Authors
  1. Li Zhang
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  2. Ahmet Yilmaz
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  3. Ri-Cheng Chian
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  4. Weon-Young Son
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  5. Xiao Yun Zhang
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  6. Dewen Kong
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  7. Michael Dahan
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  8. Hannanel Holzer
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  9. Seang Lin Tan
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  10. Asangla Ao
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Corresponding author

Correspondence to Asangla Ao.

Additional information

Capsule

Reliable preimplantation genetic diagnosis can be performed in vitrified-thawed human embryos.

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Zhang, L., Yilmaz, A., Chian, RC. et al. Reliable preimplantation genetic diagnosis in thawed human embryos vitrified at cleavage stages without biopsy. J Assist Reprod Genet 28, 597–602 (2011). https://doi.org/10.1007/s10815-011-9556-2

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  • DOI: https://doi.org/10.1007/s10815-011-9556-2

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