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Dynamic expression patterns of imprinted genes in human embryonic stem cells following prolonged passaging and differentiation

  • Stem Cell Biology
  • Published:
Journal of Assisted Reproduction and Genetics Aims and scope Submit manuscript

Abstract

Purpose

To evaluate the overall expression patterns of imprinted genes in human embryonic stem cells following long term culture and differentiation.

Materials and methods

Expression levels of 65 imprinted genes determined by PCR array were analyzed in one human embryonic stem cell line (cHES1) following prolonged passaging and differentiation.

Results

Transcripts of 63 imprinted genes were detected in cHES1 cells. Expression levels of all but 5 imprinted genes did not correlate with passage numbers or differ in cells after passage 50 compared with those before passage 50. SLC22A2, SLC22A3, CPA, H19, COPG2IT1 and IGF2 expression were significantly increased in embryoid bodies compared with undifferentiated cells.

Conclusions

The global expression profiles of imprinted genes are generally stable in human embryonic stem cells after prolonged passaging and differentiation.

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Acknowledgments

This work was supported in part by grants from the Doctoral Station Foundation, Ministry of Education (20050558097); National 863 program (20060102A1022, 2006AA02A102, 2006AA02A101); Guangdong provincial science and technology foundation (2008A030201028); National 973 program (2007CB948100); Chinese National Nature Foundation (30801239); The PhD programs foundation of ministry of China (200805581164). 2009 Sun yat-sen university sponsored programs (09YKP436).

Disclosure of Potential Conflicts of Interest

The authors indicate no potential conflicts of interest.

Author information

Authors and Affiliations

  1. Reproductive Medical Center, The First Affiliated Hospital of Sun Yat-Sen University, 58 Zhongshan 2nd Avenue, Yuexiu, 510080, Guangzhou, China

    Xiuyun Mai, Qingyun Mai, Tao Li & Canquan Zhou

Authors
  1. Xiuyun Mai
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  2. Qingyun Mai
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  3. Tao Li
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  4. Canquan Zhou
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Corresponding author

Correspondence to Canquan Zhou.

Additional information

Xiuyun Mai and Qingyun Mai contributed equally to this work.

Capsule

Transcriptional levels of imprinted genes are generally stable in human embryonic stem cells following extended culture and upon differentiation.

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Supplementary Figure 1

Comparison of housekeeping genes (HKG) expression among 3 groups (undifferentiated hES cells before passage 50, after passage 50 and differentiated hES cells). No change of expression level in 4 HKG (B2M, HPRT1, RPL13A and GAPDH) was observed. Y-value is expressed as relative fold change in mRNA levels when compared with those in cells before passage 50 defined as 1. Bars indicate mean ± SEM (n = 3). The P value were determined by ANOVA, P > 0.05 (DOC 70 kb)

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Mai, X., Mai, Q., Li, T. et al. Dynamic expression patterns of imprinted genes in human embryonic stem cells following prolonged passaging and differentiation. J Assist Reprod Genet 28, 315–323 (2011). https://doi.org/10.1007/s10815-010-9524-2

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  • DOI: https://doi.org/10.1007/s10815-010-9524-2

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