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A prospective randomized sibling-oocyte study of two media systems for culturing cleavage-stage embryos—impact on fertilization rate

  • Embryo Biology
  • Published:
Journal of Assisted Reproduction and Genetics Aims and scope Submit manuscript

Abstract

Purpose

Although several media systems have been developed, data from prospective randomised clinical studies are still lacking. In the present study we compared the effects of 2 different media systems on embryo morphology and development at days 2/3 using sibling oocytes.

Methods

In this prospective sibling-split trial, 1206 oocytes from 110 women were divided via alternate allocation to fertilization and culture in media system A (G-IVF TM v5 PLUS/ G-1TM v5 PLUS) or for fertilization and culture in media system B (Universal IVF medium/EmbryoAssist TM).

Results

The use of media system A significantly increased the normal fertilization rate (73.5% versus 67.2%; p = 0.030) and embryo utilization rate (55.5% versus 42.9%; p = 0.001), whereas polyploidy and embryo quality were similar in the two groups.

Conclusion

The different impacts on fertilization and early embryo development between the two commercially available and commonly used media systems show the importance of evaluation of the efficacy of existing sequential culture media and the need to further improve media for in vitro development of human embryos.

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Authors and Affiliations

  1. Department of Clinical Science, Intervention and Technology, Division of Obstetrics and Gynaecology, Karolinska Institutet, 141 86, Stockholm, Sweden

    Fredwell Hambiliki

  2. Department of Women’s and Children’s Health, Uppsala University, 751 85, Uppsala, Sweden

    Petra Sandell, Fatma Yaldir & Anneli Stavreus-Evers

Authors
  1. Fredwell Hambiliki
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  2. Petra Sandell
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  3. Fatma Yaldir
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  4. Anneli Stavreus-Evers
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Corresponding author

Correspondence to Anneli Stavreus-Evers.

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Capsule

Two commercially available and commonly used media systems show different impacts on fertilization and early embryo development

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Hambiliki, F., Sandell, P., Yaldir, F. et al. A prospective randomized sibling-oocyte study of two media systems for culturing cleavage-stage embryos—impact on fertilization rate. J Assist Reprod Genet 28, 335–341 (2011). https://doi.org/10.1007/s10815-010-9518-0

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  • DOI: https://doi.org/10.1007/s10815-010-9518-0

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