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Journal of Assisted Reproduction and Genetics

, Volume 28, Issue 2, pp 111–117 | Cite as

Spontaneous in vitro maturation and artificial activation of human germinal vesicle oocytes recovered from stimulated cycles

  • Laura Escrich
  • Noelia Grau
  • Amparo Mercader
  • Carmen Rubio
  • Antonio Pellicer
  • María-José EscribáEmail author
Assisted Reproduction Technologies

Abstract

Purpose

To define germinal vesicles (GV) by morphometric and morphologic examination and by chromatin compaction and to assess their spontaneous nuclear and cytoplasmic competence.

Materials

131 GV were cultured for 42.7 ± 2.4 h. Nuclear maturation was evaluated at four time points. Sixty-seven in vitro and twenty-five in vivo metaphase II (MII) were activated. Parthenotes with 2 PB and one pronucleus (NA) were studied for ploidy.

Results

A total of 74.8% GV matured to MII: 55% at 21.4 ± 2.4 h and 47.3% in the following 24 h. Artificial activation induced NA in 79.2% of in vivo-MII and in 22.4% of in vitro-MII. All NA were haploid.

Conclusions

GV spontaneously mature at the nuclear level. Their NA are haploid, but their cytoplasmic competence is compromised. Variables were not found to be predictors of oocyte competence, probably due to our population being homogeneous with respect to most of the variables studied.

Keywords

Germinal vesicle Humans Oocyte activation Spontaneous in vitro maturation 

Notes

Acknowledgments

The authors would like to thank all the IVI staff who collaborated on this project, in particular the embryologists who collected the GV immature oocytes on a daily basis, the secretaries who recruited donors and Mr. Normanly who edited this manuscript. This work was funded by IMPIVA (IMIDTG/2008/29; IMIDTF/2009/142, Generalitat Valenciana) and IVI Valencia.

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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Laura Escrich
    • 1
  • Noelia Grau
    • 1
  • Amparo Mercader
    • 1
  • Carmen Rubio
    • 1
  • Antonio Pellicer
    • 1
    • 2
  • María-José Escribá
    • 1
    • 3
    Email author
  1. 1.Universitary Institute IVI ValenciaValenciaSpain
  2. 2.Department of Paediatrics, Obstetrics and Gynaecology, University School of MedicineValencia UniversityValenciaSpain
  3. 3.Instituto Valenciano de InfertilidadInstituto Universitario IVIValenciaSpain

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