Abstract
Purpose
A laser is commonly used to remove a blastomere from an embryo for genetic testing. The laser uses intense heat which could possibly disrupt embryo development. It is the goal of this study to test the effects of different laser pulse lengths (and consequently heat) on the embryo biopsy procedure and embryo development.
Methods
Each embryo biopsy was performed randomly utilizing laser pulse lengths of 0.604mS (group I), 0.708mS (group II), and 1.010mS (group III).
Results
For groups I, II, and III, 83, 86, and 71 embryos were biopsied, respectively. There was no difference in day 5 embryo quality or lysed blastomeres between groups. Average number of blastomeres biopsied between group I (1.0 ± 0.0), II (1.0 ± 0.2), and III (1.1 ± 0.2) was significant (0.0001).
Conclusion
Our data demonstrates that laser pulse length does not influence the embryo biopsy procedure or embryo development.
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References
Rienzi L, Greco E, Ubaldi F, Iacobelli M, Martinez F, Tesarik J. Laser-assisted intracytoplasmic sperm injection. Fertil Steril. 2001;76:1045–7.
Nagy ZP, Oliveira SA, Abdelmassih V, Abdelmassih R. Novel use of laser to assist ICSI for patients with fragile oocytes: a case report. Reprod Biomed Online. 2002;4:27–31.
Ebner T, Yaman C, Moser M, Sommergruber M, Hartl J, Tews G. Laser assisted immobilization of spermatozoa prior to intracytoplasmic sperm injection in humans. Hum Reprod. 2001;16:2628–31.
Veiga A, Sandalinas M, Benkhalifa M, Boada M, Carrera M, Santalo J, et al. Laser blastocyst biopsy for preimplantation diagnosis in the human. Zygote. 1997;5:351–4.
Douglas-Hamilton DH, Conia J. Thermal effects in laser-assisted pre-embryo zona drilling. J Biomed Opt. 2001;6:205–13.
Tadir Y, Doughlas-Hamilton DH. Laser effects in the manipulation of human eggs and embryos for in vitro fertilization. Methods Cell Biol. 2007;82:409–31.
Tucker MJ, Ball GD. Assisted hatching as a technique for use in human in vitro fertilization and embryo transfer is long overdue for careful and appropriate study. J Clin Embr. 2009;12:9–14.
Rink K, Delacretaz G, Salathe RP, Senn A, Nocera D, Germond M, et al. Non contact microdrilling of mouse zona pellucida with an objective-delivered 1.48 um diode laser. Lasers Surg Med. 1996;18:52–62.
Chatzimeletiou K, Picton HM, Handyside AH. Use of non-contact, infrared laser for zona drilling of mouse embryos: assessment of immediate effects on blastomeres viability. Reprod Biomed Online. 2001;2:178–87.
Blanchet GB, Russell JB, Fincher CR, Portmann M. Laser micromanipulation in the mouse embryo: a novel approach to zona drilling. Fertil Steril. 1992;57:1337–41.
Taylor TH, Wright G, Jones-Colon S, Mitchell-Leef D, Kort HI, Nagy ZP. ICSI and IVF fertilization in patients with increased oocyte immaturity. Reprod Biomed Online. 2008;17:46–52.
Jones AE, Wright G, Kort HI, Straub RJ, Nagy ZP. Comparison of laser-assisted hatching and acidified Tyrode’s hatching by evaluation of blastocyst development rates in sibling embryos: a prospective randomized trial. Fertil Steril. 2006;85:487–91.
Joris H, De Vos A, Janssens R, Devroey P, Liebaers I, Van Steirteghem A. Comparison of the results of human embryo biopsy and outcome of PGD after zona drilling using acid Tyrode medium or a laser. Hum Reprod. 2003;18:1896–902.
Cohen J, Wells D, Munne S. Removal of 2 cells from cleavage stage embryos is likely to reduce the efficacy of chromosomal tests that are used to enhance implantation rates. Fertil Steril. 2007;87:496–503.
Hartshorn C, Anshelevich A, Wangh LJ. Laser zona drilling does not induce hsp70i transcription in blastomeres of eight-cell mouse embryos. Fertil Steril. 2005;84:1547–50.
Hartshorn C, Anshelevich A, Wangh LJ. Rapid, single-tube method for quantitative preparation and analysis of RNA and DNA in samples as small as one cell. BMC Biotechnol. 2005;5:2.
Al-Katanani YM, Hansen PJ. Induced thermotolerance in bovine two-cell embryos and the role of heat shock protein 70 in embryonic development. Mol Reprod Dev. 2002;62:174–80.
Germons M, Nocera D, Senn A, Rink K, Delacretaz G, Fakan S. Microdissection of mouse and human zona pellucida using a 1.48 um diode laser beam: efficacy and safety of the procedure. Fertil Steril. 1995;64:604–11.
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Different laser pulse lengths used during the embryo biopsy procedure do not influence the procedure or embryo development to the blastocyst stage
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Taylor, T.H., Gilchrist, J.W., Hallowell, S.V. et al. The effects of different laser pulse lengths on the embryo biopsy procedure and embryo development to the blastocyst stage. J Assist Reprod Genet 27, 663–667 (2010). https://doi.org/10.1007/s10815-010-9461-0
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DOI: https://doi.org/10.1007/s10815-010-9461-0